Significant improvement of optical traps by tuning standard water immersion objectives

Seyyed Nader Seyyed Reihani; Andrew Callum Richardson; Lene Broeng Oddershede

doi:10.1088/2040-8978/13/10/105301

Significant improvement of optical traps by tuning standard water immersion objectives

Seyyed Nader Seyyed Reihani, Andrew Callum Richardson, Lene Broeng Oddershede

17 Citationer (Scopus)

Abstract

Focused infrared lasers are widely used for micromanipulation and visualization of biological specimens. An inherent practical problem is that off-the-shelf commercial microscope objectives are designed for use with visible and not infrared wavelengths. Less aberration is introduced by water immersion objectives than by oil immersion ones, however, even water immersion objectives induce significant aberration. We present a simple method to reduce the spherical aberration induced by water immersion objectives, namely by tuning the correction collar of the objective to a value that is ∼ 10% lower than the physical thickness of the coverslip. This results in marked improvements in optical trapping strengths of up to 100% laterally and 600% axially from a standard microscope objective designed for use in the visible range. The results are generally valid for any water immersion objective with any numerical aperture.

Originalsprog	Engelsk
Tidsskrift	Journal of Optics
Vol/bind	13
Udgave nummer	10
Sider (fra-til)	105301
ISSN	0972-8821
DOI	https://doi.org/10.1088/2040-8978/13/10/105301
Status	Udgivet - 1 okt. 2011

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10.1088/2040-8978/13/10/105301

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N2 - Focused infrared lasers are widely used for micromanipulation and visualization of biological specimens. An inherent practical problem is that off-the-shelf commercial microscope objectives are designed for use with visible and not infrared wavelengths. Less aberration is introduced by water immersion objectives than by oil immersion ones, however, even water immersion objectives induce significant aberration. We present a simple method to reduce the spherical aberration induced by water immersion objectives, namely by tuning the correction collar of the objective to a value that is ∼ 10% lower than the physical thickness of the coverslip. This results in marked improvements in optical trapping strengths of up to 100% laterally and 600% axially from a standard microscope objective designed for use in the visible range. The results are generally valid for any water immersion objective with any numerical aperture.

AB - Focused infrared lasers are widely used for micromanipulation and visualization of biological specimens. An inherent practical problem is that off-the-shelf commercial microscope objectives are designed for use with visible and not infrared wavelengths. Less aberration is introduced by water immersion objectives than by oil immersion ones, however, even water immersion objectives induce significant aberration. We present a simple method to reduce the spherical aberration induced by water immersion objectives, namely by tuning the correction collar of the objective to a value that is ∼ 10% lower than the physical thickness of the coverslip. This results in marked improvements in optical trapping strengths of up to 100% laterally and 600% axially from a standard microscope objective designed for use in the visible range. The results are generally valid for any water immersion objective with any numerical aperture.

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JO - Journal of Optics (India)

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Significant improvement of optical traps by tuning standard water immersion objectives

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