Sequence/structure selective thermal and photochemical cleavage of yeast-tRNA(Phe) by UO(2)2+

Peter E. Nielsen; N E Møllegaard

doi:10.1002/(SICI)1099-1352(199605)9:3&lt;228::AID-JMR243&gt;3.0.CO;2-R

Sequence/structure selective thermal and photochemical cleavage of yeast-tRNA(Phe) by UO(2)2+

Peter E. Nielsen, N E Møllegaard

Department of Cellular and Molecular Medicine

9 Citations (Scopus)

Abstract

The uranyl(VI) ion, UO(2)2+, cleaves yeast tRNA(Phe) both thermally and photochemically. Photochemical cleavage takes place at all positions but exhibits maxima at G10, G18, G30, A38, C49 and A62. Furthermore, in the presence of stoichiometric concentrations of citrate, the cleavage is generally suppressed except that strong cleavage at positions G10 and C48-U50 persists, indicating the presence of a high-affinity metal-ion binding site. It is proposed that these photocleavage sites reflect the tertiary structure of the yeast tRNA(Phe) molecule in terms of D-loop/T-loop interaction and anticodon loop conformation and that uranyl-mediated photocleavage of RNA may be used as a probe of RNA tertiary structure, and in particular for identifying binding sites for divalent metal ions. Thus a high-affinity metal-ion binding site is inferred in the "central pocket" formed by the D-loop, and the acceptor stem.

Original language	English
Journal	Journal of Molecular Recognition
Volume	9
Issue number	3
Pages (from-to)	228-32
Number of pages	4
ISSN	0952-3499
DOIs	https://doi.org/10.1002/(SICI)1099-1352(199605)9:3<228::AID-JMR243>3.0.CO;2-R
Publication status	Published - 1997

Access to Document

10.1002/(SICI)1099-1352(199605)9:3<228::AID-JMR243>3.0.CO;2-R

Cite this

@article{86b76fa0e60011ddbf70000ea68e967b,

title = "Sequence/structure selective thermal and photochemical cleavage of yeast-tRNA(Phe) by UO(2)2+",

abstract = "The uranyl(VI) ion, UO(2)2+, cleaves yeast tRNA(Phe) both thermally and photochemically. Photochemical cleavage takes place at all positions but exhibits maxima at G10, G18, G30, A38, C49 and A62. Furthermore, in the presence of stoichiometric concentrations of citrate, the cleavage is generally suppressed except that strong cleavage at positions G10 and C48-U50 persists, indicating the presence of a high-affinity metal-ion binding site. It is proposed that these photocleavage sites reflect the tertiary structure of the yeast tRNA(Phe) molecule in terms of D-loop/T-loop interaction and anticodon loop conformation and that uranyl-mediated photocleavage of RNA may be used as a probe of RNA tertiary structure, and in particular for identifying binding sites for divalent metal ions. Thus a high-affinity metal-ion binding site is inferred in the {"}central pocket{"} formed by the D-loop, and the acceptor stem.",

author = "Nielsen, {Peter E.} and M{\o}llegaard, {N E}",

note = "Keywords: Hot Temperature; Nucleic Acid Conformation; Photochemistry; RNA, Fungal; RNA, Transfer, Phe; Saccharomyces cerevisiae; Uranium Compounds",

year = "1997",

doi = "10.1002/(SICI)1099-1352(199605)9:3<228::AID-JMR243>3.0.CO;2-R",

language = "English",

volume = "9",

pages = "228--32",

journal = "Journal of Molecular Recognition",

issn = "0952-3499",

publisher = "Wiley",

number = "3",

}

TY - JOUR

T1 - Sequence/structure selective thermal and photochemical cleavage of yeast-tRNA(Phe) by UO(2)2+

AU - Nielsen, Peter E.

AU - Møllegaard, N E

N1 - Keywords: Hot Temperature; Nucleic Acid Conformation; Photochemistry; RNA, Fungal; RNA, Transfer, Phe; Saccharomyces cerevisiae; Uranium Compounds

PY - 1997

Y1 - 1997

N2 - The uranyl(VI) ion, UO(2)2+, cleaves yeast tRNA(Phe) both thermally and photochemically. Photochemical cleavage takes place at all positions but exhibits maxima at G10, G18, G30, A38, C49 and A62. Furthermore, in the presence of stoichiometric concentrations of citrate, the cleavage is generally suppressed except that strong cleavage at positions G10 and C48-U50 persists, indicating the presence of a high-affinity metal-ion binding site. It is proposed that these photocleavage sites reflect the tertiary structure of the yeast tRNA(Phe) molecule in terms of D-loop/T-loop interaction and anticodon loop conformation and that uranyl-mediated photocleavage of RNA may be used as a probe of RNA tertiary structure, and in particular for identifying binding sites for divalent metal ions. Thus a high-affinity metal-ion binding site is inferred in the "central pocket" formed by the D-loop, and the acceptor stem.

AB - The uranyl(VI) ion, UO(2)2+, cleaves yeast tRNA(Phe) both thermally and photochemically. Photochemical cleavage takes place at all positions but exhibits maxima at G10, G18, G30, A38, C49 and A62. Furthermore, in the presence of stoichiometric concentrations of citrate, the cleavage is generally suppressed except that strong cleavage at positions G10 and C48-U50 persists, indicating the presence of a high-affinity metal-ion binding site. It is proposed that these photocleavage sites reflect the tertiary structure of the yeast tRNA(Phe) molecule in terms of D-loop/T-loop interaction and anticodon loop conformation and that uranyl-mediated photocleavage of RNA may be used as a probe of RNA tertiary structure, and in particular for identifying binding sites for divalent metal ions. Thus a high-affinity metal-ion binding site is inferred in the "central pocket" formed by the D-loop, and the acceptor stem.

U2 - 10.1002/(SICI)1099-1352(199605)9:3<228::AID-JMR243>3.0.CO;2-R

DO - 10.1002/(SICI)1099-1352(199605)9:3<228::AID-JMR243>3.0.CO;2-R

M3 - Journal article

C2 - 8938595

SN - 0952-3499

VL - 9

SP - 228

EP - 232

JO - Journal of Molecular Recognition

JF - Journal of Molecular Recognition

IS - 3

ER -

Sequence/structure selective thermal and photochemical cleavage of yeast-tRNA(Phe) by UO(2)2+

Abstract

Access to Document

Fingerprint

Cite this