Sequences of microvariant/“off-ladder” STR alleles

Eszter Rockenbauer; Magdalena Holgersson; Sarah Louise Fordyce; Maria del Carmen Avila Arcos; Claus Børsting; Anders Johannes Hansen; Rune Frank-Hansen; Eske Willerslev; Tom Gilbert; Niels Morling

doi:10.1016/j.fsigss.2011.08.102

Sequences of microvariant/“off-ladder” STR alleles

Eszter Rockenbauer, Magdalena Holgersson, Sarah Louise Fordyce, Maria del Carmen Avila Arcos, Claus Børsting, Anders Johannes Hansen, Rune Frank-Hansen, Eske Willerslev, Tom Gilbert, Niels Morling

4 Citations (Scopus)

Abstract

We have recently developed a sequencing method using a second generation sequencing platform. We typed 111 samples for one or two of 10 short tandem repeat loci and discovered a high degree of sequence diversity. Most variation was seen in the D21S11 locus and least variation was found in the D18S51 locus. Our sequencing method gave a better resolution than traditional capillary electrophoresis methods, and also better resolution than other techniques, such as pyrosequencing and mass spectrometry. We found that poly-A/T stretches longer than six bases decreased the quality of reads, making sequence composition an important factor for the quality of sequencing results.

Original language	English
Journal	Forensic Science International: Genetics Supplement Series
Volume	3
Issue number	1
Pages (from-to)	e204-e205
Number of pages	2
ISSN	1875-1768
DOIs	https://doi.org/10.1016/j.fsigss.2011.08.102
Publication status	Published - Dec 2011
Event	24th International Congress of the International Society for Forensic Genetics - Wien, Austria Duration: 30 Aug 2011 → 3 Sept 2011 Conference number: 24

Conference

Conference	24th International Congress of the International Society for Forensic Genetics
Number	24
Country/Territory	Austria
City	Wien
Period	30/08/2011 → 03/09/2011

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title = "Sequences of microvariant/“off-ladder” STR alleles",

abstract = "We have recently developed a sequencing method using a second generation sequencing platform. We typed 111 samples for one or two of 10 short tandem repeat loci and discovered a high degree of sequence diversity. Most variation was seen in the D21S11 locus and least variation was found in the D18S51 locus. Our sequencing method gave a better resolution than traditional capillary electrophoresis methods, and also better resolution than other techniques, such as pyrosequencing and mass spectrometry. We found that poly-A/T stretches longer than six bases decreased the quality of reads, making sequence composition an important factor for the quality of sequencing results.",

author = "Eszter Rockenbauer and Magdalena Holgersson and Fordyce, {Sarah Louise} and {Avila Arcos}, {Maria del Carmen} and Claus B{\o}rsting and Hansen, {Anders Johannes} and Rune Frank-Hansen and Eske Willerslev and Tom Gilbert and Niels Morling",

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doi = "10.1016/j.fsigss.2011.08.102",

language = "English",

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journal = "Forensic Science International: Genetics Supplement Series",

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T1 - Sequences of microvariant/“off-ladder” STR alleles

AU - Rockenbauer, Eszter

AU - Holgersson, Magdalena

AU - Fordyce, Sarah Louise

AU - Avila Arcos, Maria del Carmen

AU - Børsting, Claus

AU - Hansen, Anders Johannes

AU - Frank-Hansen, Rune

AU - Willerslev, Eske

AU - Gilbert, Tom

AU - Morling, Niels

N1 - Conference code: 24

PY - 2011/12

Y1 - 2011/12

N2 - We have recently developed a sequencing method using a second generation sequencing platform. We typed 111 samples for one or two of 10 short tandem repeat loci and discovered a high degree of sequence diversity. Most variation was seen in the D21S11 locus and least variation was found in the D18S51 locus. Our sequencing method gave a better resolution than traditional capillary electrophoresis methods, and also better resolution than other techniques, such as pyrosequencing and mass spectrometry. We found that poly-A/T stretches longer than six bases decreased the quality of reads, making sequence composition an important factor for the quality of sequencing results.

AB - We have recently developed a sequencing method using a second generation sequencing platform. We typed 111 samples for one or two of 10 short tandem repeat loci and discovered a high degree of sequence diversity. Most variation was seen in the D21S11 locus and least variation was found in the D18S51 locus. Our sequencing method gave a better resolution than traditional capillary electrophoresis methods, and also better resolution than other techniques, such as pyrosequencing and mass spectrometry. We found that poly-A/T stretches longer than six bases decreased the quality of reads, making sequence composition an important factor for the quality of sequencing results.

U2 - 10.1016/j.fsigss.2011.08.102

DO - 10.1016/j.fsigss.2011.08.102

M3 - Conference article

SN - 1875-1768

VL - 3

SP - e204-e205

JO - Forensic Science International: Genetics Supplement Series

JF - Forensic Science International: Genetics Supplement Series

IS - 1

T2 - 24th International Congress of the International Society for Forensic Genetics

Y2 - 30 August 2011 through 3 September 2011

ER -

Sequences of microvariant/“off-ladder” STR alleles

Abstract

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