Selective modulation of the CD4 molecular complex by Pseudomonas aeruginosa alkaline protease and elastase

B K Pedersen; A Kharazmi; T G Theander; Niels Odum; V Andersen; K Bendtzen

Selective modulation of the CD4 molecular complex by Pseudomonas aeruginosa alkaline protease and elastase

B K Pedersen, A Kharazmi, T G Theander, Niels Odum, V Andersen, K Bendtzen

16 Citations (Scopus)

Abstract

The binding of monoclonal antibodies against CD4 was specifically inhibited by treatment of human CD4+ cells with either alkaline protease (AP) or elastase (Ela), purified from Pseudomonas aeruginosa. Binding of antibodies against CD3 (pan T), CD5 (pan T), CD8 (T suppressor/cytotoxic), HLA-ABC, HLA-DR, HLA-DQ, HLA-DP/DR, and beta 2 microglobulin was not inhibited by AP or Ela. Heat-inactivation of the proteases at 65 degrees C for 20 min or treatment with the metal chelator EDTA abolished the inhibitory activity of both proteases. These findings may serve to develop novel immunological methods for the isolation and study of the lymphocyte CD4 structure, which plays an important part in the immune response.

Original language	English
Journal	Scandinavian Journal of Immunology
Volume	26
Issue number	1
Pages (from-to)	91-4
Number of pages	3
ISSN	0300-9475
Publication status	Published - 1987

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@article{113cb270a0dd11dd86a6000ea68e967b,

title = "Selective modulation of the CD4 molecular complex by Pseudomonas aeruginosa alkaline protease and elastase",

abstract = "The binding of monoclonal antibodies against CD4 was specifically inhibited by treatment of human CD4+ cells with either alkaline protease (AP) or elastase (Ela), purified from Pseudomonas aeruginosa. Binding of antibodies against CD3 (pan T), CD5 (pan T), CD8 (T suppressor/cytotoxic), HLA-ABC, HLA-DR, HLA-DQ, HLA-DP/DR, and beta 2 microglobulin was not inhibited by AP or Ela. Heat-inactivation of the proteases at 65 degrees C for 20 min or treatment with the metal chelator EDTA abolished the inhibitory activity of both proteases. These findings may serve to develop novel immunological methods for the isolation and study of the lymphocyte CD4 structure, which plays an important part in the immune response.",

author = "Pedersen, {B K} and A Kharazmi and Theander, {T G} and Niels Odum and V Andersen and K Bendtzen",

note = "Keywords: Antigens, Differentiation, T-Lymphocyte; Antigens, Surface; Endopeptidases; Humans; Pancreatic Elastase; Pseudomonas aeruginosa; Serine Endopeptidases; T-Lymphocytes",

year = "1987",

language = "English",

volume = "26",

pages = "91--4",

journal = "Scandinavian Journal of Immunology, Supplement",

issn = "0301-6323",

publisher = "Wiley-Blackwell",

number = "1",

}

TY - JOUR

T1 - Selective modulation of the CD4 molecular complex by Pseudomonas aeruginosa alkaline protease and elastase

AU - Pedersen, B K

AU - Kharazmi, A

AU - Theander, T G

AU - Odum, Niels

AU - Andersen, V

AU - Bendtzen, K

N1 - Keywords: Antigens, Differentiation, T-Lymphocyte; Antigens, Surface; Endopeptidases; Humans; Pancreatic Elastase; Pseudomonas aeruginosa; Serine Endopeptidases; T-Lymphocytes

PY - 1987

Y1 - 1987

N2 - The binding of monoclonal antibodies against CD4 was specifically inhibited by treatment of human CD4+ cells with either alkaline protease (AP) or elastase (Ela), purified from Pseudomonas aeruginosa. Binding of antibodies against CD3 (pan T), CD5 (pan T), CD8 (T suppressor/cytotoxic), HLA-ABC, HLA-DR, HLA-DQ, HLA-DP/DR, and beta 2 microglobulin was not inhibited by AP or Ela. Heat-inactivation of the proteases at 65 degrees C for 20 min or treatment with the metal chelator EDTA abolished the inhibitory activity of both proteases. These findings may serve to develop novel immunological methods for the isolation and study of the lymphocyte CD4 structure, which plays an important part in the immune response.

AB - The binding of monoclonal antibodies against CD4 was specifically inhibited by treatment of human CD4+ cells with either alkaline protease (AP) or elastase (Ela), purified from Pseudomonas aeruginosa. Binding of antibodies against CD3 (pan T), CD5 (pan T), CD8 (T suppressor/cytotoxic), HLA-ABC, HLA-DR, HLA-DQ, HLA-DP/DR, and beta 2 microglobulin was not inhibited by AP or Ela. Heat-inactivation of the proteases at 65 degrees C for 20 min or treatment with the metal chelator EDTA abolished the inhibitory activity of both proteases. These findings may serve to develop novel immunological methods for the isolation and study of the lymphocyte CD4 structure, which plays an important part in the immune response.

M3 - Journal article

C2 - 3112933

SN - 0301-6323

VL - 26

SP - 91

EP - 94

JO - Scandinavian Journal of Immunology, Supplement

JF - Scandinavian Journal of Immunology, Supplement

IS - 1

ER -

Selective modulation of the CD4 molecular complex by Pseudomonas aeruginosa alkaline protease and elastase

Abstract

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