TY - JOUR
T1 - Selective modulation of the CD4 molecular complex by Pseudomonas aeruginosa alkaline protease and elastase
AU - Pedersen, B K
AU - Kharazmi, A
AU - Theander, T G
AU - Odum, Niels
AU - Andersen, V
AU - Bendtzen, K
N1 - Keywords: Antigens, Differentiation, T-Lymphocyte; Antigens, Surface; Endopeptidases; Humans; Pancreatic Elastase; Pseudomonas aeruginosa; Serine Endopeptidases; T-Lymphocytes
PY - 1987
Y1 - 1987
N2 - The binding of monoclonal antibodies against CD4 was specifically inhibited by treatment of human CD4+ cells with either alkaline protease (AP) or elastase (Ela), purified from Pseudomonas aeruginosa. Binding of antibodies against CD3 (pan T), CD5 (pan T), CD8 (T suppressor/cytotoxic), HLA-ABC, HLA-DR, HLA-DQ, HLA-DP/DR, and beta 2 microglobulin was not inhibited by AP or Ela. Heat-inactivation of the proteases at 65 degrees C for 20 min or treatment with the metal chelator EDTA abolished the inhibitory activity of both proteases. These findings may serve to develop novel immunological methods for the isolation and study of the lymphocyte CD4 structure, which plays an important part in the immune response.
AB - The binding of monoclonal antibodies against CD4 was specifically inhibited by treatment of human CD4+ cells with either alkaline protease (AP) or elastase (Ela), purified from Pseudomonas aeruginosa. Binding of antibodies against CD3 (pan T), CD5 (pan T), CD8 (T suppressor/cytotoxic), HLA-ABC, HLA-DR, HLA-DQ, HLA-DP/DR, and beta 2 microglobulin was not inhibited by AP or Ela. Heat-inactivation of the proteases at 65 degrees C for 20 min or treatment with the metal chelator EDTA abolished the inhibitory activity of both proteases. These findings may serve to develop novel immunological methods for the isolation and study of the lymphocyte CD4 structure, which plays an important part in the immune response.
M3 - Journal article
C2 - 3112933
SN - 0301-6323
VL - 26
SP - 91
EP - 94
JO - Scandinavian Journal of Immunology, Supplement
JF - Scandinavian Journal of Immunology, Supplement
IS - 1
ER -