TY - JOUR
T1 - Selected applications of perturbed angular correlation of ¿-rays (PAC) spectroscopy in biochemistry
AU - Hemmingsen, Lars Bo Stegeager
AU - Stachura, Monika Kinga
AU - Thulstrup, Peter Waaben
AU - Christensen, Niels Johan
AU - Johnston, Karl
PY - 2010
Y1 - 2010
N2 - PAC spectroscopy was first applied in biochemistry more than 40 years ago in a study of serum albumin (Leipert et al., Nature 220:907-909, 1968). Over the following decades a number of such applications have appeared in the literature, demonstrating that PAC spectroscopy is a useful tool in the study of biomolecules. Selected prototypical examples with 111mCd, 111Ag, and 199mHg are presented in this work with a particular focus on the biological role of metal ions in catalysis, protein structure and function, protein-protein interaction in biochemical reaction paths, in vivo binding of mercury ions to barley, and the use of de novo designed proteins to systematically explore the interaction of proteins and metal ions.
AB - PAC spectroscopy was first applied in biochemistry more than 40 years ago in a study of serum albumin (Leipert et al., Nature 220:907-909, 1968). Over the following decades a number of such applications have appeared in the literature, demonstrating that PAC spectroscopy is a useful tool in the study of biomolecules. Selected prototypical examples with 111mCd, 111Ag, and 199mHg are presented in this work with a particular focus on the biological role of metal ions in catalysis, protein structure and function, protein-protein interaction in biochemical reaction paths, in vivo binding of mercury ions to barley, and the use of de novo designed proteins to systematically explore the interaction of proteins and metal ions.
U2 - 10.1007/s10751-010-0248-6
DO - 10.1007/s10751-010-0248-6
M3 - Review
SN - 0304-3843
VL - 197
SP - 255
EP - 267
JO - Hyperfine Interactions
JF - Hyperfine Interactions
IS - 1-3
ER -