Second harmonic generation microscopy investigation of the crystalline ultrastructure of three barley starch lines affected by hydration

Richard Cicek; Danielle Tokarz; Martin Steup; Ian J. Tetlow; Michael J. Emes; Kim H. Hebelstrup; Andreas Blennow; Virginijus Barzda

doi:10.1364/BOE.6.003694

Second harmonic generation microscopy investigation of the crystalline ultrastructure of three barley starch lines affected by hydration

Richard Cicek, Danielle Tokarz, Martin Steup, Ian J. Tetlow, Michael J. Emes, Kim H. Hebelstrup, Andreas Blennow, Virginijus Barzda

Section for Plant Glycobiology

16 Citations (Scopus)

125 Downloads (Pure)

Abstract

Second harmonic generation (SHG) microscopy is employed to study changes in crystalline organization due to altered gene expression and hydration in barley starch granules. SHG intensity and susceptibility ratio values (R’SHG) are obtained using reduced Stokes-Mueller polarimetric microscopy. The maximum R’SHG values occur at moderate moisture indicating the narrowest orientation distribution of nonlinear dipoles from the cylindrical axis of glucan helices. The maximum SHG intensity occurs at the highest moisture and amylopectin content. These results support the hypothesis that SHG is caused by ordered hydrogen and hydroxyl bond networks which increase with hydration of starch granules.

Original language	English
Journal	Biomedical Optics Express
Volume	6
Issue number	10
Pages (from-to)	3694-3700
Number of pages	7
ISSN	2156-7085
DOIs	https://doi.org/10.1364/BOE.6.003694
Publication status	Published - 2015

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@article{19b7878fae574bf3a299be913c15ca31,

title = "Second harmonic generation microscopy investigation of the crystalline ultrastructure of three barley starch lines affected by hydration",

abstract = "Second harmonic generation (SHG) microscopy is employed to study changes in crystalline organization due to altered gene expression and hydration in barley starch granules. SHG intensity and susceptibility ratio values (R{\textquoteright}SHG) are obtained using reduced Stokes-Mueller polarimetric microscopy. The maximum R{\textquoteright}SHG values occur at moderate moisture indicating the narrowest orientation distribution of nonlinear dipoles from the cylindrical axis of glucan helices. The maximum SHG intensity occurs at the highest moisture and amylopectin content. These results support the hypothesis that SHG is caused by ordered hydrogen and hydroxyl bond networks which increase with hydration of starch granules.",

author = "Richard Cicek and Danielle Tokarz and Martin Steup and Tetlow, {Ian J.} and Emes, {Michael J.} and Hebelstrup, {Kim H.} and Andreas Blennow and Virginijus Barzda",

year = "2015",

doi = "10.1364/BOE.6.003694",

language = "English",

volume = "6",

pages = "3694--3700",

journal = "Biomedical Optics Express",

issn = "2156-7085",

publisher = "Optical Society of America",

number = "10",

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TY - JOUR

T1 - Second harmonic generation microscopy investigation of the crystalline ultrastructure of three barley starch lines affected by hydration

AU - Cicek, Richard

AU - Tokarz, Danielle

AU - Steup, Martin

AU - Tetlow, Ian J.

AU - Emes, Michael J.

AU - Hebelstrup, Kim H.

AU - Blennow, Andreas

AU - Barzda, Virginijus

PY - 2015

Y1 - 2015

N2 - Second harmonic generation (SHG) microscopy is employed to study changes in crystalline organization due to altered gene expression and hydration in barley starch granules. SHG intensity and susceptibility ratio values (R’SHG) are obtained using reduced Stokes-Mueller polarimetric microscopy. The maximum R’SHG values occur at moderate moisture indicating the narrowest orientation distribution of nonlinear dipoles from the cylindrical axis of glucan helices. The maximum SHG intensity occurs at the highest moisture and amylopectin content. These results support the hypothesis that SHG is caused by ordered hydrogen and hydroxyl bond networks which increase with hydration of starch granules.

AB - Second harmonic generation (SHG) microscopy is employed to study changes in crystalline organization due to altered gene expression and hydration in barley starch granules. SHG intensity and susceptibility ratio values (R’SHG) are obtained using reduced Stokes-Mueller polarimetric microscopy. The maximum R’SHG values occur at moderate moisture indicating the narrowest orientation distribution of nonlinear dipoles from the cylindrical axis of glucan helices. The maximum SHG intensity occurs at the highest moisture and amylopectin content. These results support the hypothesis that SHG is caused by ordered hydrogen and hydroxyl bond networks which increase with hydration of starch granules.

U2 - 10.1364/BOE.6.003694

DO - 10.1364/BOE.6.003694

M3 - Journal article

C2 - 26504621

SN - 2156-7085

VL - 6

SP - 3694

EP - 3700

JO - Biomedical Optics Express

JF - Biomedical Optics Express

IS - 10

ER -

Second harmonic generation microscopy investigation of the crystalline ultrastructure of three barley starch lines affected by hydration

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