Savinase action on bovine serum albumin (BSA) monolayers demonstrated with measurements at the air-water interface and liquid Atomic Force Microscopy (AFM) imaging

Konstantin Balashev; Thomas H Callisen; Allan Svendsen; Thomas Bjørnholm

doi:10.1016/j.colsurfb.2011.07.043

Savinase action on bovine serum albumin (BSA) monolayers demonstrated with measurements at the air-water interface and liquid Atomic Force Microscopy (AFM) imaging

Konstantin Balashev, Thomas H Callisen, Allan Svendsen, Thomas Bjørnholm

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9 Citations (Scopus)

Abstract

We studied the enzymatic action of Savinase on bovine serum albumin (BSA) organized in a monolayer spread at the air/water interface or adsorbed at the mica surface. We carried out two types of experiments. In the first one we followed the degradation of the protein monolayer by measuring the surface pressure and surface area decrease versus time. In the second approach we applied AFM imaging of the supported BSA monolayers adsorbed on mica solid supports and extracted information for the enzyme action by analyzing the obtained images of the surface topography in the course of enzyme action. In both cases we obtained an estimate for the turnover number (TON) of the enzyme reaction.

Original language	English
Journal	Colloids and Surfaces B: Biointerfaces
Volume	88
Issue number	2
Pages (from-to)	582-6
Number of pages	5
ISSN	0927-7765
DOIs	https://doi.org/10.1016/j.colsurfb.2011.07.043
Publication status	Published - 1 Dec 2011

Keywords

Air
Animals
Cattle
Microscopy, Atomic Force
Serine Endopeptidases
Serum Albumin, Bovine
Water

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10.1016/j.colsurfb.2011.07.043

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Savinase action on bovine serum albumin (BSA) monolayers demonstrated with measurements at the air-water interface and liquid Atomic Force Microscopy (AFM) imaging. / Balashev, Konstantin; Callisen, Thomas H; Svendsen, Allan et al.

In: Colloids and Surfaces B: Biointerfaces, Vol. 88, No. 2, 01.12.2011, p. 582-6.

Research output: Contribution to journal › Journal article › Research › peer-review

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abstract = "We studied the enzymatic action of Savinase on bovine serum albumin (BSA) organized in a monolayer spread at the air/water interface or adsorbed at the mica surface. We carried out two types of experiments. In the first one we followed the degradation of the protein monolayer by measuring the surface pressure and surface area decrease versus time. In the second approach we applied AFM imaging of the supported BSA monolayers adsorbed on mica solid supports and extracted information for the enzyme action by analyzing the obtained images of the surface topography in the course of enzyme action. In both cases we obtained an estimate for the turnover number (TON) of the enzyme reaction.",

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AU - Svendsen, Allan

AU - Bjørnholm, Thomas

PY - 2011/12/1

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N2 - We studied the enzymatic action of Savinase on bovine serum albumin (BSA) organized in a monolayer spread at the air/water interface or adsorbed at the mica surface. We carried out two types of experiments. In the first one we followed the degradation of the protein monolayer by measuring the surface pressure and surface area decrease versus time. In the second approach we applied AFM imaging of the supported BSA monolayers adsorbed on mica solid supports and extracted information for the enzyme action by analyzing the obtained images of the surface topography in the course of enzyme action. In both cases we obtained an estimate for the turnover number (TON) of the enzyme reaction.

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KW - Air

KW - Animals

KW - Cattle

KW - Microscopy, Atomic Force

KW - Serine Endopeptidases

KW - Serum Albumin, Bovine

KW - Water

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JO - Colloids and Surfaces B: Biointerfaces

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ER -

Savinase action on bovine serum albumin (BSA) monolayers demonstrated with measurements at the air-water interface and liquid Atomic Force Microscopy (AFM) imaging

Abstract

Keywords

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