Savinase action on bovine serum albumin (BSA) monolayers demonstrated with measurements at the air-water interface and liquid Atomic Force Microscopy (AFM) imaging

Konstantin Balashev; Thomas H Callisen; Allan Svendsen; Thomas Bjørnholm

doi:10.1016/j.colsurfb.2011.07.043

Savinase action on bovine serum albumin (BSA) monolayers demonstrated with measurements at the air-water interface and liquid Atomic Force Microscopy (AFM) imaging

Konstantin Balashev, Thomas H Callisen, Allan Svendsen, Thomas Bjørnholm

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9 Citationer (Scopus)

Abstract

We studied the enzymatic action of Savinase on bovine serum albumin (BSA) organized in a monolayer spread at the air/water interface or adsorbed at the mica surface. We carried out two types of experiments. In the first one we followed the degradation of the protein monolayer by measuring the surface pressure and surface area decrease versus time. In the second approach we applied AFM imaging of the supported BSA monolayers adsorbed on mica solid supports and extracted information for the enzyme action by analyzing the obtained images of the surface topography in the course of enzyme action. In both cases we obtained an estimate for the turnover number (TON) of the enzyme reaction.

Originalsprog	Engelsk
Tidsskrift	Colloids and Surfaces B: Biointerfaces
Vol/bind	88
Udgave nummer	2
Sider (fra-til)	582-6
Antal sider	5
ISSN	0927-7765
DOI	https://doi.org/10.1016/j.colsurfb.2011.07.043
Status	Udgivet - 1 dec. 2011

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10.1016/j.colsurfb.2011.07.043

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Savinase action on bovine serum albumin (BSA) monolayers demonstrated with measurements at the air-water interface and liquid Atomic Force Microscopy (AFM) imaging. / Balashev, Konstantin; Callisen, Thomas H; Svendsen, Allan et al.

I: Colloids and Surfaces B: Biointerfaces, Bind 88, Nr. 2, 01.12.2011, s. 582-6.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

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abstract = "We studied the enzymatic action of Savinase on bovine serum albumin (BSA) organized in a monolayer spread at the air/water interface or adsorbed at the mica surface. We carried out two types of experiments. In the first one we followed the degradation of the protein monolayer by measuring the surface pressure and surface area decrease versus time. In the second approach we applied AFM imaging of the supported BSA monolayers adsorbed on mica solid supports and extracted information for the enzyme action by analyzing the obtained images of the surface topography in the course of enzyme action. In both cases we obtained an estimate for the turnover number (TON) of the enzyme reaction.",

keywords = "Air, Animals, Cattle, Microscopy, Atomic Force, Serine Endopeptidases, Serum Albumin, Bovine, Water",

author = "Konstantin Balashev and Callisen, {Thomas H} and Allan Svendsen and Thomas Bj{\o}rnholm",

year = "2011",

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doi = "10.1016/j.colsurfb.2011.07.043",

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T1 - Savinase action on bovine serum albumin (BSA) monolayers demonstrated with measurements at the air-water interface and liquid Atomic Force Microscopy (AFM) imaging

AU - Balashev, Konstantin

AU - Callisen, Thomas H

AU - Svendsen, Allan

AU - Bjørnholm, Thomas

PY - 2011/12/1

Y1 - 2011/12/1

N2 - We studied the enzymatic action of Savinase on bovine serum albumin (BSA) organized in a monolayer spread at the air/water interface or adsorbed at the mica surface. We carried out two types of experiments. In the first one we followed the degradation of the protein monolayer by measuring the surface pressure and surface area decrease versus time. In the second approach we applied AFM imaging of the supported BSA monolayers adsorbed on mica solid supports and extracted information for the enzyme action by analyzing the obtained images of the surface topography in the course of enzyme action. In both cases we obtained an estimate for the turnover number (TON) of the enzyme reaction.

AB - We studied the enzymatic action of Savinase on bovine serum albumin (BSA) organized in a monolayer spread at the air/water interface or adsorbed at the mica surface. We carried out two types of experiments. In the first one we followed the degradation of the protein monolayer by measuring the surface pressure and surface area decrease versus time. In the second approach we applied AFM imaging of the supported BSA monolayers adsorbed on mica solid supports and extracted information for the enzyme action by analyzing the obtained images of the surface topography in the course of enzyme action. In both cases we obtained an estimate for the turnover number (TON) of the enzyme reaction.

KW - Air

KW - Animals

KW - Cattle

KW - Microscopy, Atomic Force

KW - Serine Endopeptidases

KW - Serum Albumin, Bovine

KW - Water

U2 - 10.1016/j.colsurfb.2011.07.043

DO - 10.1016/j.colsurfb.2011.07.043

M3 - Journal article

C2 - 21868205

SN - 0927-7765

VL - 88

SP - 582

EP - 586

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

IS - 2

ER -

Savinase action on bovine serum albumin (BSA) monolayers demonstrated with measurements at the air-water interface and liquid Atomic Force Microscopy (AFM) imaging

Abstract

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