Roles of type IV pili, flagellum-mediated motility and extracellular DNA in the formation of mature multicellular structures in Pseudomonas aeruginosa biofilms

Kim Bundvig Barken; Sünje Johanna Pamp; Liang Yang; Morten Gjermansen; Jacob J. Bertrand; Mikkel Klausen; Michael Givskov; Cynthia B. Whitchurch; Joanne N. Engel; Tim Tolker-Nielsen

doi:10.1111/j.1462-2920.2008.01658.x

Roles of type IV pili, flagellum-mediated motility and extracellular DNA in the formation of mature multicellular structures in Pseudomonas aeruginosa biofilms

Kim Bundvig Barken, Sünje Johanna Pamp, Liang Yang, Morten Gjermansen, Jacob J. Bertrand, Mikkel Klausen, Michael Givskov, Cynthia B. Whitchurch, Joanne N. Engel, Tim Tolker-Nielsen

240 Citations (Scopus)

Abstract

When grown as a biofilm in laboratory flow chambers Pseudomonas aeruginosa can develop mushroom-shaped multicellular structures consisting of distinct subpopulations in the cap and stalk portions. We have previously presented evidence that formation of the cap portion of the mushroom-shaped structures in P. aeruginosa biofilms occurs via bacterial migration and depends on type IV pili (Mol Microbiol 50: 61-68). In the present study we examine additional factors involved in the formation of this multicellular substructure. While pilA mutants, lacking type IV pili, are deficient in mushroom cap formation, pilH and chpA mutants, which are inactivated in the type IV pili-linked chemosensory system, showed only minor defects in cap formation. On the contrary, fliM mutants, which are non-flagellated, and cheY mutants, which are inactivated in the flagellum-linked chemotaxis system, were largely deficient in cap formation. Experiments involving DNase treatment of developing biofilms provided evidence that extracellular DNA plays a role in cap formation. Moreover, mutants that are deficient in quorum sensing-controlled DNA release formed microcolonies upon which wild-type bacteria could not form caps. These results constitute evidence that type IV pili, flagellum-mediated motility and quorum sensing-controlled DNA release are involved in the formation of mature multicellular structures in P. aeruginosa biofilms.

Original language	English
Journal	Environmental Microbiology
Volume	10
Issue number	9
Pages (from-to)	2331-2343
Number of pages	13
ISSN	1462-2912
DOIs	https://doi.org/10.1111/j.1462-2920.2008.01658.x
Publication status	Published - 2008
Externally published	Yes

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Barken, K. B., Pamp, S. J., Yang, L., Gjermansen, M., Bertrand, J. J., Klausen, M., Givskov, M., Whitchurch, C. B., Engel, J. N., & Tolker-Nielsen, T. (2008). Roles of type IV pili, flagellum-mediated motility and extracellular DNA in the formation of mature multicellular structures in Pseudomonas aeruginosa biofilms. Environmental Microbiology, 10(9), 2331-2343. https://doi.org/10.1111/j.1462-2920.2008.01658.x

Roles of type IV pili, flagellum-mediated motility and extracellular DNA in the formation of mature multicellular structures in Pseudomonas aeruginosa biofilms. / Barken, Kim Bundvig; Pamp, Sünje Johanna; Yang, Liang et al.
In: Environmental Microbiology, Vol. 10, No. 9, 2008, p. 2331-2343.

Research output: Contribution to journal › Journal article › Research › peer-review

Barken, KB, Pamp, SJ, Yang, L, Gjermansen, M, Bertrand, JJ, Klausen, M, Givskov, M, Whitchurch, CB, Engel, JN & Tolker-Nielsen, T 2008, 'Roles of type IV pili, flagellum-mediated motility and extracellular DNA in the formation of mature multicellular structures in Pseudomonas aeruginosa biofilms', Environmental Microbiology, vol. 10, no. 9, pp. 2331-2343. https://doi.org/10.1111/j.1462-2920.2008.01658.x

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title = "Roles of type IV pili, flagellum-mediated motility and extracellular DNA in the formation of mature multicellular structures in Pseudomonas aeruginosa biofilms",

abstract = "When grown as a biofilm in laboratory flow chambers Pseudomonas aeruginosa can develop mushroom-shaped multicellular structures consisting of distinct subpopulations in the cap and stalk portions. We have previously presented evidence that formation of the cap portion of the mushroom-shaped structures in P. aeruginosa biofilms occurs via bacterial migration and depends on type IV pili (Mol Microbiol 50: 61-68). In the present study we examine additional factors involved in the formation of this multicellular substructure. While pilA mutants, lacking type IV pili, are deficient in mushroom cap formation, pilH and chpA mutants, which are inactivated in the type IV pili-linked chemosensory system, showed only minor defects in cap formation. On the contrary, fliM mutants, which are non-flagellated, and cheY mutants, which are inactivated in the flagellum-linked chemotaxis system, were largely deficient in cap formation. Experiments involving DNase treatment of developing biofilms provided evidence that extracellular DNA plays a role in cap formation. Moreover, mutants that are deficient in quorum sensing-controlled DNA release formed microcolonies upon which wild-type bacteria could not form caps. These results constitute evidence that type IV pili, flagellum-mediated motility and quorum sensing-controlled DNA release are involved in the formation of mature multicellular structures in P. aeruginosa biofilms.",

author = "Barken, {Kim Bundvig} and Pamp, {S{\"u}nje Johanna} and Liang Yang and Morten Gjermansen and Bertrand, {Jacob J.} and Mikkel Klausen and Michael Givskov and Whitchurch, {Cynthia B.} and Engel, {Joanne N.} and Tim Tolker-Nielsen",

note = "Keywords: Bacterial Adhesion; Biofilms; Chemotaxis; DNA, Bacterial; Extracellular Matrix; Fimbriae Proteins; Fimbriae, Bacterial; Flagella; Genes, Bacterial; Genetic Complementation Test; Genotype; Microscopy, Confocal; Pseudomonas aeruginosa; Quorum Sensing",

year = "2008",

doi = "10.1111/j.1462-2920.2008.01658.x",

language = "English",

volume = "10",

pages = "2331--2343",

journal = "Environmental Microbiology",

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T1 - Roles of type IV pili, flagellum-mediated motility and extracellular DNA in the formation of mature multicellular structures in Pseudomonas aeruginosa biofilms

AU - Barken, Kim Bundvig

AU - Pamp, Sünje Johanna

AU - Yang, Liang

AU - Gjermansen, Morten

AU - Bertrand, Jacob J.

AU - Klausen, Mikkel

AU - Givskov, Michael

AU - Whitchurch, Cynthia B.

AU - Engel, Joanne N.

AU - Tolker-Nielsen, Tim

N1 - Keywords: Bacterial Adhesion; Biofilms; Chemotaxis; DNA, Bacterial; Extracellular Matrix; Fimbriae Proteins; Fimbriae, Bacterial; Flagella; Genes, Bacterial; Genetic Complementation Test; Genotype; Microscopy, Confocal; Pseudomonas aeruginosa; Quorum Sensing

PY - 2008

Y1 - 2008

N2 - When grown as a biofilm in laboratory flow chambers Pseudomonas aeruginosa can develop mushroom-shaped multicellular structures consisting of distinct subpopulations in the cap and stalk portions. We have previously presented evidence that formation of the cap portion of the mushroom-shaped structures in P. aeruginosa biofilms occurs via bacterial migration and depends on type IV pili (Mol Microbiol 50: 61-68). In the present study we examine additional factors involved in the formation of this multicellular substructure. While pilA mutants, lacking type IV pili, are deficient in mushroom cap formation, pilH and chpA mutants, which are inactivated in the type IV pili-linked chemosensory system, showed only minor defects in cap formation. On the contrary, fliM mutants, which are non-flagellated, and cheY mutants, which are inactivated in the flagellum-linked chemotaxis system, were largely deficient in cap formation. Experiments involving DNase treatment of developing biofilms provided evidence that extracellular DNA plays a role in cap formation. Moreover, mutants that are deficient in quorum sensing-controlled DNA release formed microcolonies upon which wild-type bacteria could not form caps. These results constitute evidence that type IV pili, flagellum-mediated motility and quorum sensing-controlled DNA release are involved in the formation of mature multicellular structures in P. aeruginosa biofilms.

AB - When grown as a biofilm in laboratory flow chambers Pseudomonas aeruginosa can develop mushroom-shaped multicellular structures consisting of distinct subpopulations in the cap and stalk portions. We have previously presented evidence that formation of the cap portion of the mushroom-shaped structures in P. aeruginosa biofilms occurs via bacterial migration and depends on type IV pili (Mol Microbiol 50: 61-68). In the present study we examine additional factors involved in the formation of this multicellular substructure. While pilA mutants, lacking type IV pili, are deficient in mushroom cap formation, pilH and chpA mutants, which are inactivated in the type IV pili-linked chemosensory system, showed only minor defects in cap formation. On the contrary, fliM mutants, which are non-flagellated, and cheY mutants, which are inactivated in the flagellum-linked chemotaxis system, were largely deficient in cap formation. Experiments involving DNase treatment of developing biofilms provided evidence that extracellular DNA plays a role in cap formation. Moreover, mutants that are deficient in quorum sensing-controlled DNA release formed microcolonies upon which wild-type bacteria could not form caps. These results constitute evidence that type IV pili, flagellum-mediated motility and quorum sensing-controlled DNA release are involved in the formation of mature multicellular structures in P. aeruginosa biofilms.

U2 - 10.1111/j.1462-2920.2008.01658.x

DO - 10.1111/j.1462-2920.2008.01658.x

M3 - Journal article

C2 - 18485000

SN - 1462-2912

VL - 10

SP - 2331

EP - 2343

JO - Environmental Microbiology

JF - Environmental Microbiology

IS - 9

ER -

Roles of type IV pili, flagellum-mediated motility and extracellular DNA in the formation of mature multicellular structures in Pseudomonas aeruginosa biofilms

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