RNA/DNA co-analysis from human skin and contact traces – results of a sixth collaborative EDNAP exercise

C Haas; E Hanson; R Banemann; A M Bento; A Berti; Á Carracedo; C Courts; G De Cock; K Drobnic; R Fleming; C Franchi; I Gomes; G Hadzic; S A Harbison; Benjamin Benn Hjort; C Hollard; P Hoff-Olsen; C Keyser; A Kondili; O Maroñas; N McCallum; P Miniati; Niels Morling; H Niederstätter; F Noël; W Parson; M J Porto; A D Roeder; E Sauer; P M Schneider; G Shanthan; T Sijen; D Syndercombe Court; M Turanská; M van den Berge; M Vennemann; A Vidaki; L Zatkalíková; J Ballantyne

doi:10.1016/j.fsigen.2015.01.002

RNA/DNA co-analysis from human skin and contact traces – results of a sixth collaborative EDNAP exercise

C Haas, E Hanson, R Banemann, A M Bento, A Berti, Á Carracedo, C Courts, G De Cock, K Drobnic, R Fleming, C Franchi, I Gomes, G Hadzic, S A Harbison, Benjamin Benn Hjort, C Hollard, P Hoff-Olsen, C Keyser, A Kondili, O MaroñasN McCallum, P Miniati, Niels Morling, H Niederstätter, F Noël, W Parson, M J Porto, A D Roeder, E Sauer, P M Schneider, G Shanthan, T Sijen, D Syndercombe Court, M Turanská, M van den Berge, M Vennemann, A Vidaki, L Zatkalíková, J Ballantyne

32 Citations (Scopus)

Abstract

The European DNA profiling group (EDNAP) organized a sixth collaborative exercise on RNA/DNA co-analysis for body fluid/tissue identification and STR profiling. The task was to identify skin samples/contact traces using specific RNA biomarkers and test three housekeeping genes for their suitability as reference genes. Eight stains, a skin RNA dilution series and, optionally, bona fide or mock casework samples of human or non-human origin were analyzed by 22 participating laboratories using RNA extraction or RNA/DNA co-extraction methods. Two sets of previously described skin-specific markers were used: skin1 pentaplex (LCE1C, LCE1D, LCE2D, IL1F7 and CCL27) and skin2 triplex (LOR, KRT9 and CDSN) in conjunction with a housekeeping gene, HKG, triplex (B2M, UBC and UCE). The laboratories used different chemistries and instrumentation. All laboratories were able to successfully isolate and detect mRNA in contact traces (e.g., human skin, palm-, hand- and fingerprints, clothing, car interiors, computer accessories and electronic devices). The simultaneous extraction of RNA and DNA provides an opportunity for positive identification of the tissue source of origin by mRNA profiling as well as a simultaneous identification of the body fluid donor by STR profiling. The skin markers LCE1C and LOR and the housekeeping gene marker B2M were detected in the majority of contact traces. Detection of the other markers was inconsistent, possibly due to the low amounts and/or poor quality of the genetic material present in shed skin cells. The results of this and the previous collaborative RNA exercises support RNA profiling as a reliable body fluid/tissue identification method that can easily be combined with current STR typing technology.

Original language	English
Journal	Forensic Science International: Genetics
Volume	16
Pages (from-to)	139-147
Number of pages	9
ISSN	1872-4973
DOIs	https://doi.org/10.1016/j.fsigen.2015.01.002
Publication status	Published - May 2015

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Haas, C., Hanson, E., Banemann, R., Bento, A. M., Berti, A., Carracedo, Á., Courts, C., De Cock, G., Drobnic, K., Fleming, R., Franchi, C., Gomes, I., Hadzic, G., Harbison, S. A., Hjort, B. B., Hollard, C., Hoff-Olsen, P., Keyser, C., Kondili, A., ... Ballantyne, J. (2015). RNA/DNA co-analysis from human skin and contact traces – results of a sixth collaborative EDNAP exercise. Forensic Science International: Genetics, 16, 139-147. https://doi.org/10.1016/j.fsigen.2015.01.002

Haas, C, Hanson, E, Banemann, R, Bento, AM, Berti, A, Carracedo, Á, Courts, C, De Cock, G, Drobnic, K, Fleming, R, Franchi, C, Gomes, I, Hadzic, G, Harbison, SA, Hjort, BB, Hollard, C, Hoff-Olsen, P, Keyser, C, Kondili, A, Maroñas, O, McCallum, N, Miniati, P, Morling, N, Niederstätter, H, Noël, F, Parson, W, Porto, MJ, Roeder, AD, Sauer, E, Schneider, PM, Shanthan, G, Sijen, T, Syndercombe Court, D, Turanská, M, van den Berge, M, Vennemann, M, Vidaki, A, Zatkalíková, L & Ballantyne, J 2015, 'RNA/DNA co-analysis from human skin and contact traces – results of a sixth collaborative EDNAP exercise', Forensic Science International: Genetics, vol. 16, pp. 139-147. https://doi.org/10.1016/j.fsigen.2015.01.002

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title = "RNA/DNA co-analysis from human skin and contact traces – results of a sixth collaborative EDNAP exercise",

abstract = "The European DNA profiling group (EDNAP) organized a sixth collaborative exercise on RNA/DNA co-analysis for body fluid/tissue identification and STR profiling. The task was to identify skin samples/contact traces using specific RNA biomarkers and test three housekeeping genes for their suitability as reference genes. Eight stains, a skin RNA dilution series and, optionally, bona fide or mock casework samples of human or non-human origin were analyzed by 22 participating laboratories using RNA extraction or RNA/DNA co-extraction methods. Two sets of previously described skin-specific markers were used: skin1 pentaplex (LCE1C, LCE1D, LCE2D, IL1F7 and CCL27) and skin2 triplex (LOR, KRT9 and CDSN) in conjunction with a housekeeping gene, HKG, triplex (B2M, UBC and UCE). The laboratories used different chemistries and instrumentation. All laboratories were able to successfully isolate and detect mRNA in contact traces (e.g., human skin, palm-, hand- and fingerprints, clothing, car interiors, computer accessories and electronic devices). The simultaneous extraction of RNA and DNA provides an opportunity for positive identification of the tissue source of origin by mRNA profiling as well as a simultaneous identification of the body fluid donor by STR profiling. The skin markers LCE1C and LOR and the housekeeping gene marker B2M were detected in the majority of contact traces. Detection of the other markers was inconsistent, possibly due to the low amounts and/or poor quality of the genetic material present in shed skin cells. The results of this and the previous collaborative RNA exercises support RNA profiling as a reliable body fluid/tissue identification method that can easily be combined with current STR typing technology.",

author = "C Haas and E Hanson and R Banemann and Bento, {A M} and A Berti and {\'A} Carracedo and C Courts and {De Cock}, G and K Drobnic and R Fleming and C Franchi and I Gomes and G Hadzic and Harbison, {S A} and Hjort, {Benjamin Benn} and C Hollard and P Hoff-Olsen and C Keyser and A Kondili and O Maro{\~n}as and N McCallum and P Miniati and Niels Morling and H Niederst{\"a}tter and F No{\"e}l and W Parson and Porto, {M J} and Roeder, {A D} and E Sauer and Schneider, {P M} and G Shanthan and T Sijen and {Syndercombe Court}, D and M Turansk{\'a} and {van den Berge}, M and M Vennemann and A Vidaki and L Zatkal{\'i}kov{\'a} and J Ballantyne",

year = "2015",

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doi = "10.1016/j.fsigen.2015.01.002",

language = "English",

volume = "16",

pages = "139--147",

journal = "Forensic Science International: Genetics",

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T1 - RNA/DNA co-analysis from human skin and contact traces – results of a sixth collaborative EDNAP exercise

AU - Haas, C

AU - Hanson, E

AU - Banemann, R

AU - Bento, A M

AU - Berti, A

AU - Carracedo, Á

AU - Courts, C

AU - De Cock, G

AU - Drobnic, K

AU - Fleming, R

AU - Franchi, C

AU - Gomes, I

AU - Hadzic, G

AU - Harbison, S A

AU - Hjort, Benjamin Benn

AU - Hollard, C

AU - Hoff-Olsen, P

AU - Keyser, C

AU - Kondili, A

AU - Maroñas, O

AU - McCallum, N

AU - Miniati, P

AU - Morling, Niels

AU - Niederstätter, H

AU - Noël, F

AU - Parson, W

AU - Porto, M J

AU - Roeder, A D

AU - Sauer, E

AU - Schneider, P M

AU - Shanthan, G

AU - Sijen, T

AU - Syndercombe Court, D

AU - Turanská, M

AU - van den Berge, M

AU - Vennemann, M

AU - Vidaki, A

AU - Zatkalíková, L

AU - Ballantyne, J

PY - 2015/5

Y1 - 2015/5

N2 - The European DNA profiling group (EDNAP) organized a sixth collaborative exercise on RNA/DNA co-analysis for body fluid/tissue identification and STR profiling. The task was to identify skin samples/contact traces using specific RNA biomarkers and test three housekeeping genes for their suitability as reference genes. Eight stains, a skin RNA dilution series and, optionally, bona fide or mock casework samples of human or non-human origin were analyzed by 22 participating laboratories using RNA extraction or RNA/DNA co-extraction methods. Two sets of previously described skin-specific markers were used: skin1 pentaplex (LCE1C, LCE1D, LCE2D, IL1F7 and CCL27) and skin2 triplex (LOR, KRT9 and CDSN) in conjunction with a housekeeping gene, HKG, triplex (B2M, UBC and UCE). The laboratories used different chemistries and instrumentation. All laboratories were able to successfully isolate and detect mRNA in contact traces (e.g., human skin, palm-, hand- and fingerprints, clothing, car interiors, computer accessories and electronic devices). The simultaneous extraction of RNA and DNA provides an opportunity for positive identification of the tissue source of origin by mRNA profiling as well as a simultaneous identification of the body fluid donor by STR profiling. The skin markers LCE1C and LOR and the housekeeping gene marker B2M were detected in the majority of contact traces. Detection of the other markers was inconsistent, possibly due to the low amounts and/or poor quality of the genetic material present in shed skin cells. The results of this and the previous collaborative RNA exercises support RNA profiling as a reliable body fluid/tissue identification method that can easily be combined with current STR typing technology.

AB - The European DNA profiling group (EDNAP) organized a sixth collaborative exercise on RNA/DNA co-analysis for body fluid/tissue identification and STR profiling. The task was to identify skin samples/contact traces using specific RNA biomarkers and test three housekeeping genes for their suitability as reference genes. Eight stains, a skin RNA dilution series and, optionally, bona fide or mock casework samples of human or non-human origin were analyzed by 22 participating laboratories using RNA extraction or RNA/DNA co-extraction methods. Two sets of previously described skin-specific markers were used: skin1 pentaplex (LCE1C, LCE1D, LCE2D, IL1F7 and CCL27) and skin2 triplex (LOR, KRT9 and CDSN) in conjunction with a housekeeping gene, HKG, triplex (B2M, UBC and UCE). The laboratories used different chemistries and instrumentation. All laboratories were able to successfully isolate and detect mRNA in contact traces (e.g., human skin, palm-, hand- and fingerprints, clothing, car interiors, computer accessories and electronic devices). The simultaneous extraction of RNA and DNA provides an opportunity for positive identification of the tissue source of origin by mRNA profiling as well as a simultaneous identification of the body fluid donor by STR profiling. The skin markers LCE1C and LOR and the housekeeping gene marker B2M were detected in the majority of contact traces. Detection of the other markers was inconsistent, possibly due to the low amounts and/or poor quality of the genetic material present in shed skin cells. The results of this and the previous collaborative RNA exercises support RNA profiling as a reliable body fluid/tissue identification method that can easily be combined with current STR typing technology.

U2 - 10.1016/j.fsigen.2015.01.002

DO - 10.1016/j.fsigen.2015.01.002

M3 - Journal article

C2 - 25600397

SN - 1872-4973

VL - 16

SP - 139

EP - 147

JO - Forensic Science International: Genetics

JF - Forensic Science International: Genetics

ER -

RNA/DNA co-analysis from human skin and contact traces – results of a sixth collaborative EDNAP exercise

Abstract

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