RNA/DNA co-analysis from human menstrual blood and vaginal secretion stains: Results of a fourth and fifth collaborative EDNAP exercise

Claus Haas; E Hanson; M J Anjos; K N Ballantyne; R Banemann; B Bhoelai; E Borges; M Carvalho; C Courts; G De Cock; K Drobnic; M Dötsch; R Fleming; C Franchi; I Gomes; G Hadzic; S A Harbison; J Harteveld; Benjamin Benn Hjort; C Hollard; P Hoff-Olsen; C Hüls; C Keyser; O Maroñas; N McCallum; D Moore; Niels Morling; H Niederstätter; F Noël; W Parson; C Phillips; C Popielarz; A D Roeder; L Salvaderi; E Sauer; Peter M. Schneider; G Shanthan; D Syndercombe Court; M Turanská; R A H van Oorschot; M Vennemann; A Vidaki; L Zatkalíková; J Ballantyne

doi:10.1016/j.fsigen.2013.09.009

RNA/DNA co-analysis from human menstrual blood and vaginal secretion stains: Results of a fourth and fifth collaborative EDNAP exercise

Claus Haas, E Hanson, M J Anjos, K N Ballantyne, R Banemann, B Bhoelai, E Borges, M Carvalho, C Courts, G De Cock, K Drobnic, M Dötsch, R Fleming, C Franchi, I Gomes, G Hadzic, S A Harbison, J Harteveld, Benjamin Benn Hjort, C HollardP Hoff-Olsen, C Hüls, C Keyser, O Maroñas, N McCallum, D Moore, Niels Morling, H Niederstätter, F Noël, W Parson, C Phillips, C Popielarz, A D Roeder, L Salvaderi, E Sauer, Peter M. Schneider, G Shanthan, D Syndercombe Court, M Turanská, R A H van Oorschot, M Vennemann, A Vidaki, L Zatkalíková, J Ballantyne

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Abstract

The European DNA Profiling Group (EDNAP) organized a fourth and fifth collaborative exercise on RNA/DNA co-analysis for body fluid identification and STR profiling. The task was to identify dried menstrual blood and vaginal secretion stains using specific RNA biomarkers, and additionally test 3 housekeeping genes for their suitability as reference genes. Six menstrual blood and six vaginal secretion stains, two dilution series (1/4-1/64 pieces of a menstrual blood/vaginal swab) and, optionally, bona fide or mock casework samples of human or non-human origin were analyzed by 24 participating laboratories, using RNA extraction or RNA/DNA co-extraction methods. Two novel menstrual blood mRNA multiplexes were used: MMP triplex (MMP7, MMP10, MMP11) and MB triplex (MSX1, LEFTY2, SFRP4) in conjunction with a housekeeping gene triplex (B2M, UBC, UCE). Two novel mRNA multiplexes and a HBD1 singleplex were used for the identification of vaginal secretion: Vag triplex (MYOZ1, CYP2B7P1 and MUC4) and a Lactobacillus-specific Lacto triplex (Ljen, Lcris, Lgas). The laboratories used different chemistries and instrumentation and all were able to successfully isolate and detect mRNA in dried stains. The simultaneous extraction of RNA and DNA allowed for positive identification of the tissue/fluid source of origin by mRNA profiling as well as a simultaneous identification of the body fluid donor by STR profiling, also from old and compromised casework samples. The results of this and the previous collaborative RNA exercises support RNA profiling as a reliable body fluid identification method that can easily be combined with current STR typing technology.

Original language	English
Journal	Forensic science international. Genetics
Volume	8
Issue number	1
Pages (from-to)	203-12
Number of pages	10
DOIs	https://doi.org/10.1016/j.fsigen.2013.09.009
Publication status	Published - Jan 2014

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10.1016/j.fsigen.2013.09.009

RNA-DNA co-analysis from human menstrual blood and vaginal secretion stains - Results of a fourth and fifth collaborative EDNAP exercise

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Haas, C., Hanson, E., Anjos, M. J., Ballantyne, K. N., Banemann, R., Bhoelai, B., Borges, E., Carvalho, M., Courts, C., De Cock, G., Drobnic, K., Dötsch, M., Fleming, R., Franchi, C., Gomes, I., Hadzic, G., Harbison, S. A., Harteveld, J., Hjort, B. B., ... Ballantyne, J. (2014). RNA/DNA co-analysis from human menstrual blood and vaginal secretion stains: Results of a fourth and fifth collaborative EDNAP exercise. Forensic science international. Genetics, 8(1), 203-12. https://doi.org/10.1016/j.fsigen.2013.09.009

Haas, C, Hanson, E, Anjos, MJ, Ballantyne, KN, Banemann, R, Bhoelai, B, Borges, E, Carvalho, M, Courts, C, De Cock, G, Drobnic, K, Dötsch, M, Fleming, R, Franchi, C, Gomes, I, Hadzic, G, Harbison, SA, Harteveld, J, Hjort, BB, Hollard, C, Hoff-Olsen, P, Hüls, C, Keyser, C, Maroñas, O, McCallum, N, Moore, D, Morling, N, Niederstätter, H, Noël, F, Parson, W, Phillips, C, Popielarz, C, Roeder, AD, Salvaderi, L, Sauer, E, Schneider, PM, Shanthan, G, Court, DS, Turanská, M, van Oorschot, RAH, Vennemann, M, Vidaki, A, Zatkalíková, L & Ballantyne, J 2014, 'RNA/DNA co-analysis from human menstrual blood and vaginal secretion stains: Results of a fourth and fifth collaborative EDNAP exercise', Forensic science international. Genetics, vol. 8, no. 1, pp. 203-12. https://doi.org/10.1016/j.fsigen.2013.09.009

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title = "RNA/DNA co-analysis from human menstrual blood and vaginal secretion stains: Results of a fourth and fifth collaborative EDNAP exercise",

abstract = "The European DNA Profiling Group (EDNAP) organized a fourth and fifth collaborative exercise on RNA/DNA co-analysis for body fluid identification and STR profiling. The task was to identify dried menstrual blood and vaginal secretion stains using specific RNA biomarkers, and additionally test 3 housekeeping genes for their suitability as reference genes. Six menstrual blood and six vaginal secretion stains, two dilution series (1/4-1/64 pieces of a menstrual blood/vaginal swab) and, optionally, bona fide or mock casework samples of human or non-human origin were analyzed by 24 participating laboratories, using RNA extraction or RNA/DNA co-extraction methods. Two novel menstrual blood mRNA multiplexes were used: MMP triplex (MMP7, MMP10, MMP11) and MB triplex (MSX1, LEFTY2, SFRP4) in conjunction with a housekeeping gene triplex (B2M, UBC, UCE). Two novel mRNA multiplexes and a HBD1 singleplex were used for the identification of vaginal secretion: Vag triplex (MYOZ1, CYP2B7P1 and MUC4) and a Lactobacillus-specific Lacto triplex (Ljen, Lcris, Lgas). The laboratories used different chemistries and instrumentation and all were able to successfully isolate and detect mRNA in dried stains. The simultaneous extraction of RNA and DNA allowed for positive identification of the tissue/fluid source of origin by mRNA profiling as well as a simultaneous identification of the body fluid donor by STR profiling, also from old and compromised casework samples. The results of this and the previous collaborative RNA exercises support RNA profiling as a reliable body fluid identification method that can easily be combined with current STR typing technology.",

author = "Claus Haas and E Hanson and Anjos, {M J} and Ballantyne, {K N} and R Banemann and B Bhoelai and E Borges and M Carvalho and C Courts and {De Cock}, G and K Drobnic and M D{\"o}tsch and R Fleming and C Franchi and I Gomes and G Hadzic and Harbison, {S A} and J Harteveld and Hjort, {Benjamin Benn} and C Hollard and P Hoff-Olsen and C H{\"u}ls and C Keyser and O Maro{\~n}as and N McCallum and D Moore and Niels Morling and H Niederst{\"a}tter and F No{\"e}l and W Parson and C Phillips and C Popielarz and Roeder, {A D} and L Salvaderi and E Sauer and Schneider, {Peter M.} and G Shanthan and Court, {D Syndercombe} and M Turansk{\'a} and {van Oorschot}, {R A H} and M Vennemann and A Vidaki and L Zatkal{\'i}kov{\'a} and J Ballantyne",

year = "2014",

month = jan,

doi = "10.1016/j.fsigen.2013.09.009",

language = "English",

volume = "8",

pages = "203--12",

journal = "Forensic Science International: Genetics",

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T1 - RNA/DNA co-analysis from human menstrual blood and vaginal secretion stains

T2 - Results of a fourth and fifth collaborative EDNAP exercise

AU - Haas, Claus

AU - Hanson, E

AU - Anjos, M J

AU - Ballantyne, K N

AU - Banemann, R

AU - Bhoelai, B

AU - Borges, E

AU - Carvalho, M

AU - Courts, C

AU - De Cock, G

AU - Drobnic, K

AU - Dötsch, M

AU - Fleming, R

AU - Franchi, C

AU - Gomes, I

AU - Hadzic, G

AU - Harbison, S A

AU - Harteveld, J

AU - Hjort, Benjamin Benn

AU - Hollard, C

AU - Hoff-Olsen, P

AU - Hüls, C

AU - Keyser, C

AU - Maroñas, O

AU - McCallum, N

AU - Moore, D

AU - Morling, Niels

AU - Niederstätter, H

AU - Noël, F

AU - Parson, W

AU - Phillips, C

AU - Popielarz, C

AU - Roeder, A D

AU - Salvaderi, L

AU - Sauer, E

AU - Schneider, Peter M.

AU - Shanthan, G

AU - Court, D Syndercombe

AU - Turanská, M

AU - van Oorschot, R A H

AU - Vennemann, M

AU - Vidaki, A

AU - Zatkalíková, L

AU - Ballantyne, J

PY - 2014/1

Y1 - 2014/1

N2 - The European DNA Profiling Group (EDNAP) organized a fourth and fifth collaborative exercise on RNA/DNA co-analysis for body fluid identification and STR profiling. The task was to identify dried menstrual blood and vaginal secretion stains using specific RNA biomarkers, and additionally test 3 housekeeping genes for their suitability as reference genes. Six menstrual blood and six vaginal secretion stains, two dilution series (1/4-1/64 pieces of a menstrual blood/vaginal swab) and, optionally, bona fide or mock casework samples of human or non-human origin were analyzed by 24 participating laboratories, using RNA extraction or RNA/DNA co-extraction methods. Two novel menstrual blood mRNA multiplexes were used: MMP triplex (MMP7, MMP10, MMP11) and MB triplex (MSX1, LEFTY2, SFRP4) in conjunction with a housekeeping gene triplex (B2M, UBC, UCE). Two novel mRNA multiplexes and a HBD1 singleplex were used for the identification of vaginal secretion: Vag triplex (MYOZ1, CYP2B7P1 and MUC4) and a Lactobacillus-specific Lacto triplex (Ljen, Lcris, Lgas). The laboratories used different chemistries and instrumentation and all were able to successfully isolate and detect mRNA in dried stains. The simultaneous extraction of RNA and DNA allowed for positive identification of the tissue/fluid source of origin by mRNA profiling as well as a simultaneous identification of the body fluid donor by STR profiling, also from old and compromised casework samples. The results of this and the previous collaborative RNA exercises support RNA profiling as a reliable body fluid identification method that can easily be combined with current STR typing technology.

AB - The European DNA Profiling Group (EDNAP) organized a fourth and fifth collaborative exercise on RNA/DNA co-analysis for body fluid identification and STR profiling. The task was to identify dried menstrual blood and vaginal secretion stains using specific RNA biomarkers, and additionally test 3 housekeeping genes for their suitability as reference genes. Six menstrual blood and six vaginal secretion stains, two dilution series (1/4-1/64 pieces of a menstrual blood/vaginal swab) and, optionally, bona fide or mock casework samples of human or non-human origin were analyzed by 24 participating laboratories, using RNA extraction or RNA/DNA co-extraction methods. Two novel menstrual blood mRNA multiplexes were used: MMP triplex (MMP7, MMP10, MMP11) and MB triplex (MSX1, LEFTY2, SFRP4) in conjunction with a housekeeping gene triplex (B2M, UBC, UCE). Two novel mRNA multiplexes and a HBD1 singleplex were used for the identification of vaginal secretion: Vag triplex (MYOZ1, CYP2B7P1 and MUC4) and a Lactobacillus-specific Lacto triplex (Ljen, Lcris, Lgas). The laboratories used different chemistries and instrumentation and all were able to successfully isolate and detect mRNA in dried stains. The simultaneous extraction of RNA and DNA allowed for positive identification of the tissue/fluid source of origin by mRNA profiling as well as a simultaneous identification of the body fluid donor by STR profiling, also from old and compromised casework samples. The results of this and the previous collaborative RNA exercises support RNA profiling as a reliable body fluid identification method that can easily be combined with current STR typing technology.

U2 - 10.1016/j.fsigen.2013.09.009

DO - 10.1016/j.fsigen.2013.09.009

M3 - Journal article

C2 - 24315610

SN - 1872-4973

VL - 8

SP - 203

EP - 212

JO - Forensic Science International: Genetics

JF - Forensic Science International: Genetics

IS - 1

ER -

RNA/DNA co-analysis from human menstrual blood and vaginal secretion stains: Results of a fourth and fifth collaborative EDNAP exercise

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