Abstract
Two RP-HPLC systems were developed for the separation of the products of the conversion of proinsulin into insulin in rat and mouse islets, including proinsulin I and II. Peaks were identified by microsequencing and radiosequencing. It was confirmed that mouse C-peptide I has a two amino acid deletion compared to rat C-peptide I. A marked species difference in the ratio between insulin I and II was observed, i.e., 2:1 in the rat and 1:2 in the mouse. Pulse-chase experiments in rat islets have demonstrated that the ratio between insulin I and II in newly synthesized insulin is higher than that of the stored insulin, indicating a slower conversion rate of proinsulin II compared to proinsulin I.
| Original language | English |
|---|---|
| Journal | Journal of Chromatography A |
| Volume | 462 |
| Pages (from-to) | 243-54 |
| Number of pages | 12 |
| ISSN | 0021-9673 |
| Publication status | Published - 13 Jan 1989 |
Keywords
- Alleles
- Amino Acid Sequence
- Animals
- C-Peptide
- Chromatography, High Pressure Liquid
- Insulin
- Islets of Langerhans
- Mice
- Mice, Inbred Strains
- Molecular Sequence Data
- Proinsulin
- Rats
- Rats, Inbred Strains