Reversed-phase high-performance liquid chromatographic analyses of insulin biosynthesis in isolated rat and mouse islets

S Linde; Bruno A. Hansen; B S Welinder; Jens Høiriis Nielsen

Reversed-phase high-performance liquid chromatographic analyses of insulin biosynthesis in isolated rat and mouse islets

S Linde, Bruno A. Hansen, B S Welinder, Jens Høiriis Nielsen

Inflammation, Metabolism and Oxidation

19 Citationer (Scopus)

Abstract

Two RP-HPLC systems were developed for the separation of the products of the conversion of proinsulin into insulin in rat and mouse islets, including proinsulin I and II. Peaks were identified by microsequencing and radiosequencing. It was confirmed that mouse C-peptide I has a two amino acid deletion compared to rat C-peptide I. A marked species difference in the ratio between insulin I and II was observed, i.e., 2:1 in the rat and 1:2 in the mouse. Pulse-chase experiments in rat islets have demonstrated that the ratio between insulin I and II in newly synthesized insulin is higher than that of the stored insulin, indicating a slower conversion rate of proinsulin II compared to proinsulin I.

Originalsprog	Engelsk
Tidsskrift	Journal of Chromatography A
Vol/bind	462
Sider (fra-til)	243-54
Antal sider	12
ISSN	0021-9673
Status	Udgivet - 13 jan. 1989

Citationsformater

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title = "Reversed-phase high-performance liquid chromatographic analyses of insulin biosynthesis in isolated rat and mouse islets",

abstract = "Two RP-HPLC systems were developed for the separation of the products of the conversion of proinsulin into insulin in rat and mouse islets, including proinsulin I and II. Peaks were identified by microsequencing and radiosequencing. It was confirmed that mouse C-peptide I has a two amino acid deletion compared to rat C-peptide I. A marked species difference in the ratio between insulin I and II was observed, i.e., 2:1 in the rat and 1:2 in the mouse. Pulse-chase experiments in rat islets have demonstrated that the ratio between insulin I and II in newly synthesized insulin is higher than that of the stored insulin, indicating a slower conversion rate of proinsulin II compared to proinsulin I.",

keywords = "Alleles, Amino Acid Sequence, Animals, C-Peptide, Chromatography, High Pressure Liquid, Insulin, Islets of Langerhans, Mice, Mice, Inbred Strains, Molecular Sequence Data, Proinsulin, Rats, Rats, Inbred Strains",

author = "S Linde and Hansen, {Bruno A.} and Welinder, {B S} and Nielsen, {Jens H{\o}iriis}",

year = "1989",

month = jan,

day = "13",

language = "English",

volume = "462",

pages = "243--54",

journal = "Journal of Chromatography",

issn = "0301-4770",

publisher = "Elsevier",

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TY - JOUR

T1 - Reversed-phase high-performance liquid chromatographic analyses of insulin biosynthesis in isolated rat and mouse islets

AU - Linde, S

AU - Hansen, Bruno A.

AU - Welinder, B S

AU - Nielsen, Jens Høiriis

PY - 1989/1/13

Y1 - 1989/1/13

N2 - Two RP-HPLC systems were developed for the separation of the products of the conversion of proinsulin into insulin in rat and mouse islets, including proinsulin I and II. Peaks were identified by microsequencing and radiosequencing. It was confirmed that mouse C-peptide I has a two amino acid deletion compared to rat C-peptide I. A marked species difference in the ratio between insulin I and II was observed, i.e., 2:1 in the rat and 1:2 in the mouse. Pulse-chase experiments in rat islets have demonstrated that the ratio between insulin I and II in newly synthesized insulin is higher than that of the stored insulin, indicating a slower conversion rate of proinsulin II compared to proinsulin I.

AB - Two RP-HPLC systems were developed for the separation of the products of the conversion of proinsulin into insulin in rat and mouse islets, including proinsulin I and II. Peaks were identified by microsequencing and radiosequencing. It was confirmed that mouse C-peptide I has a two amino acid deletion compared to rat C-peptide I. A marked species difference in the ratio between insulin I and II was observed, i.e., 2:1 in the rat and 1:2 in the mouse. Pulse-chase experiments in rat islets have demonstrated that the ratio between insulin I and II in newly synthesized insulin is higher than that of the stored insulin, indicating a slower conversion rate of proinsulin II compared to proinsulin I.

KW - Alleles

KW - Amino Acid Sequence

KW - Animals

KW - C-Peptide

KW - Chromatography, High Pressure Liquid

KW - Insulin

KW - Islets of Langerhans

KW - Mice

KW - Mice, Inbred Strains

KW - Molecular Sequence Data

KW - Proinsulin

KW - Rats

KW - Rats, Inbred Strains

M3 - Journal article

C2 - 2661585

SN - 0301-4770

VL - 462

SP - 243

EP - 254

JO - Journal of Chromatography

JF - Journal of Chromatography

ER -

Reversed-phase high-performance liquid chromatographic analyses of insulin biosynthesis in isolated rat and mouse islets

Abstract

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