Results of a collaborative study of the EDNAP group regarding the reproducibility and robustness of the Y-chromosome STRs DYS19, DYS389 I and II, DYS390 and DYS393 in a PCR pentaplex format

A Carracedo; A Beckmann; A Bengs; B Brinkmann; A Caglia; C Capelli; P Gill; L Gusmão; C Hagelberg; C Hohoff; B Hoste; A Kihlgren; A Kloosterman; B Myhre Dupuy; N Morling; G O'Donnell; W Parson; C Phillips; M Pouwels; R Scheithauer; H Schmitter; Peter M Schneider; J Schumm; I Skitsa; B Stradmann-Bellinghausen; M Stuart; Denise Syndercombe-Court; C Vide

Results of a collaborative study of the EDNAP group regarding the reproducibility and robustness of the Y-chromosome STRs DYS19, DYS389 I and II, DYS390 and DYS393 in a PCR pentaplex format

A Carracedo, A Beckmann, A Bengs, B Brinkmann, A Caglia, C Capelli, P Gill, L Gusmão, C Hagelberg, C Hohoff, B Hoste, A Kihlgren, A Kloosterman, B Myhre Dupuy, N Morling, G O'Donnell, W Parson, C Phillips, M Pouwels, R ScheithauerH Schmitter, Peter M Schneider, J Schumm, I Skitsa, B Stradmann-Bellinghausen, M Stuart, Denise Syndercombe-Court, C Vide

Section of Forensic Genetics

35 Citations (Scopus)

Abstract

A collaborative exercise was carried out by the European DNA Profiling Group (EDNAP) in the frame work of the STADNAP program, i.e. standardization of DNA profiling in Europe, in order to evaluate the performance of a Y-chromosome STR pentaplex, which includes the loci DYS19, DYS389 I and II, DYS390 and DYS393 and to determine whether uniformity of results could be achieved among different European laboratories.Laboratories were asked to analyze the five Y-STRs using singleplex and multiplex conditions in three bloodstains and one mixed stain (95% female and 5% male).All the laboratories reported the same results even for the mixed stain included in the exercise. This demonstrates the reproducibility and robustness of Y-chromosome STR typing even with multiplex formats and proves the usefulness of Y-STR systems for analyzing mixed stains with a male component.A total of 930 male samples from 10 different populations from Europe were also analysed for all the loci included in the pentaplex. Eight of these ten populations also included haplotype data.As for single gene analysis, haplotype diversity was higher in Germany and Italy and lower in Western European countries and Finland.Pairwise haplotype analysis shows the Finnish departure from the rest of the populations and a relatively homogeneity in the other European populations with F(ST) estimates lower than 0.05.UPGMA analysis shows an association of Western European population (Ireland, UK, Portugal and Galicia) on the one hand and central European populations on the other.

Original language	English
Journal	Forensic Science International
Volume	119
Issue number	1
Pages (from-to)	28-41
Number of pages	13
ISSN	0379-0738
Publication status	Published - 2001

Cite this

Carracedo, A., Beckmann, A., Bengs, A., Brinkmann, B., Caglia, A., Capelli, C., Gill, P., Gusmão, L., Hagelberg, C., Hohoff, C., Hoste, B., Kihlgren, A., Kloosterman, A., Myhre Dupuy, B., Morling, N., O'Donnell, G., Parson, W., Phillips, C., Pouwels, M., ... Vide, C. (2001). Results of a collaborative study of the EDNAP group regarding the reproducibility and robustness of the Y-chromosome STRs DYS19, DYS389 I and II, DYS390 and DYS393 in a PCR pentaplex format. Forensic Science International, 119(1), 28-41.

Results of a collaborative study of the EDNAP group regarding the reproducibility and robustness of the Y-chromosome STRs DYS19, DYS389 I and II, DYS390 and DYS393 in a PCR pentaplex format. / Carracedo, A; Beckmann, A; Bengs, A et al.

In: Forensic Science International, Vol. 119, No. 1, 2001, p. 28-41.

Research output: Contribution to journal › Journal article › Research › peer-review

Carracedo, A, Beckmann, A, Bengs, A, Brinkmann, B, Caglia, A, Capelli, C, Gill, P, Gusmão, L, Hagelberg, C, Hohoff, C, Hoste, B, Kihlgren, A, Kloosterman, A, Myhre Dupuy, B, Morling, N, O'Donnell, G, Parson, W, Phillips, C, Pouwels, M, Scheithauer, R, Schmitter, H, Schneider, PM, Schumm, J, Skitsa, I, Stradmann-Bellinghausen, B, Stuart, M, Syndercombe-Court, D & Vide, C 2001, 'Results of a collaborative study of the EDNAP group regarding the reproducibility and robustness of the Y-chromosome STRs DYS19, DYS389 I and II, DYS390 and DYS393 in a PCR pentaplex format', Forensic Science International, vol. 119, no. 1, pp. 28-41.

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title = "Results of a collaborative study of the EDNAP group regarding the reproducibility and robustness of the Y-chromosome STRs DYS19, DYS389 I and II, DYS390 and DYS393 in a PCR pentaplex format",

abstract = "A collaborative exercise was carried out by the European DNA Profiling Group (EDNAP) in the frame work of the STADNAP program, i.e. standardization of DNA profiling in Europe, in order to evaluate the performance of a Y-chromosome STR pentaplex, which includes the loci DYS19, DYS389 I and II, DYS390 and DYS393 and to determine whether uniformity of results could be achieved among different European laboratories.Laboratories were asked to analyze the five Y-STRs using singleplex and multiplex conditions in three bloodstains and one mixed stain (95% female and 5% male).All the laboratories reported the same results even for the mixed stain included in the exercise. This demonstrates the reproducibility and robustness of Y-chromosome STR typing even with multiplex formats and proves the usefulness of Y-STR systems for analyzing mixed stains with a male component.A total of 930 male samples from 10 different populations from Europe were also analysed for all the loci included in the pentaplex. Eight of these ten populations also included haplotype data.As for single gene analysis, haplotype diversity was higher in Germany and Italy and lower in Western European countries and Finland.Pairwise haplotype analysis shows the Finnish departure from the rest of the populations and a relatively homogeneity in the other European populations with F(ST) estimates lower than 0.05.UPGMA analysis shows an association of Western European population (Ireland, UK, Portugal and Galicia) on the one hand and central European populations on the other.",

author = "A Carracedo and A Beckmann and A Bengs and B Brinkmann and A Caglia and C Capelli and P Gill and L Gusm{\~a}o and C Hagelberg and C Hohoff and B Hoste and A Kihlgren and A Kloosterman and {Myhre Dupuy}, B and N Morling and G O'Donnell and W Parson and C Phillips and M Pouwels and R Scheithauer and H Schmitter and Schneider, {Peter M} and J Schumm and I Skitsa and B Stradmann-Bellinghausen and M Stuart and Denise Syndercombe-Court and C Vide",

note = "Keywords: Blood Stains; Cooperative Behavior; DNA Fingerprinting; Europe; Female; Gene Frequency; Genetic Variation; Haplotypes; Humans; Interinstitutional Relations; Laboratories; Male; Minisatellite Repeats; Polymerase Chain Reaction; Polymorphism, Genetic; Reference Standards; Y Chromosome",

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T1 - Results of a collaborative study of the EDNAP group regarding the reproducibility and robustness of the Y-chromosome STRs DYS19, DYS389 I and II, DYS390 and DYS393 in a PCR pentaplex format

AU - Carracedo, A

AU - Beckmann, A

AU - Bengs, A

AU - Brinkmann, B

AU - Caglia, A

AU - Capelli, C

AU - Gill, P

AU - Gusmão, L

AU - Hagelberg, C

AU - Hohoff, C

AU - Hoste, B

AU - Kihlgren, A

AU - Kloosterman, A

AU - Myhre Dupuy, B

AU - Morling, N

AU - O'Donnell, G

AU - Parson, W

AU - Phillips, C

AU - Pouwels, M

AU - Scheithauer, R

AU - Schmitter, H

AU - Schneider, Peter M

AU - Schumm, J

AU - Skitsa, I

AU - Stradmann-Bellinghausen, B

AU - Stuart, M

AU - Syndercombe-Court, Denise

AU - Vide, C

N1 - Keywords: Blood Stains; Cooperative Behavior; DNA Fingerprinting; Europe; Female; Gene Frequency; Genetic Variation; Haplotypes; Humans; Interinstitutional Relations; Laboratories; Male; Minisatellite Repeats; Polymerase Chain Reaction; Polymorphism, Genetic; Reference Standards; Y Chromosome

PY - 2001

Y1 - 2001

N2 - A collaborative exercise was carried out by the European DNA Profiling Group (EDNAP) in the frame work of the STADNAP program, i.e. standardization of DNA profiling in Europe, in order to evaluate the performance of a Y-chromosome STR pentaplex, which includes the loci DYS19, DYS389 I and II, DYS390 and DYS393 and to determine whether uniformity of results could be achieved among different European laboratories.Laboratories were asked to analyze the five Y-STRs using singleplex and multiplex conditions in three bloodstains and one mixed stain (95% female and 5% male).All the laboratories reported the same results even for the mixed stain included in the exercise. This demonstrates the reproducibility and robustness of Y-chromosome STR typing even with multiplex formats and proves the usefulness of Y-STR systems for analyzing mixed stains with a male component.A total of 930 male samples from 10 different populations from Europe were also analysed for all the loci included in the pentaplex. Eight of these ten populations also included haplotype data.As for single gene analysis, haplotype diversity was higher in Germany and Italy and lower in Western European countries and Finland.Pairwise haplotype analysis shows the Finnish departure from the rest of the populations and a relatively homogeneity in the other European populations with F(ST) estimates lower than 0.05.UPGMA analysis shows an association of Western European population (Ireland, UK, Portugal and Galicia) on the one hand and central European populations on the other.

AB - A collaborative exercise was carried out by the European DNA Profiling Group (EDNAP) in the frame work of the STADNAP program, i.e. standardization of DNA profiling in Europe, in order to evaluate the performance of a Y-chromosome STR pentaplex, which includes the loci DYS19, DYS389 I and II, DYS390 and DYS393 and to determine whether uniformity of results could be achieved among different European laboratories.Laboratories were asked to analyze the five Y-STRs using singleplex and multiplex conditions in three bloodstains and one mixed stain (95% female and 5% male).All the laboratories reported the same results even for the mixed stain included in the exercise. This demonstrates the reproducibility and robustness of Y-chromosome STR typing even with multiplex formats and proves the usefulness of Y-STR systems for analyzing mixed stains with a male component.A total of 930 male samples from 10 different populations from Europe were also analysed for all the loci included in the pentaplex. Eight of these ten populations also included haplotype data.As for single gene analysis, haplotype diversity was higher in Germany and Italy and lower in Western European countries and Finland.Pairwise haplotype analysis shows the Finnish departure from the rest of the populations and a relatively homogeneity in the other European populations with F(ST) estimates lower than 0.05.UPGMA analysis shows an association of Western European population (Ireland, UK, Portugal and Galicia) on the one hand and central European populations on the other.

M3 - Journal article

C2 - 11348791

SN - 0379-0738

VL - 119

SP - 28

EP - 41

JO - Forensic Science International

JF - Forensic Science International

IS - 1

ER -