Report of the European DNA Profiling Group (EDNAP)--an investigation of the hypervariable STR loci ACTBP2, APOAI1 and D11S554 and the compound loci D12S391 and D1S1656

P Gill; E d'Aloja; B Dupuy; Birthe Eriksen; M Jangblad; V Johnsson; A D Kloosterman; A Kratzer; M V Lareu; B Mevag; N Morling; C Phillips; H Pfitzinger; S Rand; M Sabatier; R Scheithauer; H Schmitter; Peter M. Schneider; I Skitsa; M C Vide

Report of the European DNA Profiling Group (EDNAP)--an investigation of the hypervariable STR loci ACTBP2, APOAI1 and D11S554 and the compound loci D12S391 and D1S1656

P Gill, E d'Aloja, B Dupuy, Birthe Eriksen, M Jangblad, V Johnsson, A D Kloosterman, A Kratzer, M V Lareu, B Mevag, N Morling, C Phillips, H Pfitzinger, S Rand, M Sabatier, R Scheithauer, H Schmitter, Peter M. Schneider, I Skitsa, M C Vide

15 Citations (Scopus)

Abstract

This paper describes the results of three collaborative exercises which continues the EDNAP theme to explore whether uniformity of DNA profiling results could be achieved between European laboratories using STRs. In an earlier exercise, complex hypervariable AAAG-repeat STR loci were investigated, but reproducibility was found to be poor because of the variation of techniques used by participating laboratories. In the exercise reported here, an internal allelic ladder composed of ACTBP2 and D11S554 fragments was distributed. This ladder was used to size ACTBP2 analysed by a "singleplex" PCR amplification and D11S554 combined with APOAI1 in a separate "duplex" reaction. Laboratories were asked to test 7 blood stains, one of which was a known control, and to report the results to the co-ordinating laboratory. The exercise demonstrated that ACTBP2 showed good reproducibility between laboratories, whereas further testing would be needed to validate APOAI1 and D11S554 for interlaboratory comparisons. In separate exercises, the simple loci D12S391 and D1S1656 were tested; both of these showed excellent reproducibility between laboratories.

Original language	English
Journal	Forensic Science International
Volume	98
Issue number	3
Pages (from-to)	193-200
ISSN	0379-0738
Publication status	Published - 1998

Cite this

Gill, P., d'Aloja, E., Dupuy, B., Eriksen, B., Jangblad, M., Johnsson, V., Kloosterman, A. D., Kratzer, A., Lareu, M. V., Mevag, B., Morling, N., Phillips, C., Pfitzinger, H., Rand, S., Sabatier, M., Scheithauer, R., Schmitter, H., Schneider, P. M., Skitsa, I., & Vide, M. C. (1998). Report of the European DNA Profiling Group (EDNAP)--an investigation of the hypervariable STR loci ACTBP2, APOAI1 and D11S554 and the compound loci D12S391 and D1S1656. Forensic Science International, 98(3), 193-200.

Gill, P, d'Aloja, E, Dupuy, B, Eriksen, B, Jangblad, M, Johnsson, V, Kloosterman, AD, Kratzer, A, Lareu, MV, Mevag, B, Morling, N, Phillips, C, Pfitzinger, H, Rand, S, Sabatier, M, Scheithauer, R, Schmitter, H, Schneider, PM, Skitsa, I & Vide, MC 1998, 'Report of the European DNA Profiling Group (EDNAP)--an investigation of the hypervariable STR loci ACTBP2, APOAI1 and D11S554 and the compound loci D12S391 and D1S1656', Forensic Science International, vol. 98, no. 3, pp. 193-200.

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title = "Report of the European DNA Profiling Group (EDNAP)--an investigation of the hypervariable STR loci ACTBP2, APOAI1 and D11S554 and the compound loci D12S391 and D1S1656",

abstract = "This paper describes the results of three collaborative exercises which continues the EDNAP theme to explore whether uniformity of DNA profiling results could be achieved between European laboratories using STRs. In an earlier exercise, complex hypervariable AAAG-repeat STR loci were investigated, but reproducibility was found to be poor because of the variation of techniques used by participating laboratories. In the exercise reported here, an internal allelic ladder composed of ACTBP2 and D11S554 fragments was distributed. This ladder was used to size ACTBP2 analysed by a {"}singleplex{"} PCR amplification and D11S554 combined with APOAI1 in a separate {"}duplex{"} reaction. Laboratories were asked to test 7 blood stains, one of which was a known control, and to report the results to the co-ordinating laboratory. The exercise demonstrated that ACTBP2 showed good reproducibility between laboratories, whereas further testing would be needed to validate APOAI1 and D11S554 for interlaboratory comparisons. In separate exercises, the simple loci D12S391 and D1S1656 were tested; both of these showed excellent reproducibility between laboratories.",

author = "P Gill and E d'Aloja and B Dupuy and Birthe Eriksen and M Jangblad and V Johnsson and Kloosterman, {A D} and A Kratzer and Lareu, {M V} and B Mevag and N Morling and C Phillips and H Pfitzinger and S Rand and M Sabatier and R Scheithauer and H Schmitter and Schneider, {Peter M.} and I Skitsa and Vide, {M C}",

note = "Keywords: Alleles; DNA Fingerprinting; DNA, Satellite; Europe; Humans; Immunoglobulin Variable Region; International Cooperation; Minisatellite Repeats; Polymerase Chain Reaction; Reproducibility of Results; Societies, Medical",

year = "1998",

language = "English",

volume = "98",

pages = "193--200",

journal = "Forensic Science International",

issn = "0379-0738",

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T1 - Report of the European DNA Profiling Group (EDNAP)--an investigation of the hypervariable STR loci ACTBP2, APOAI1 and D11S554 and the compound loci D12S391 and D1S1656

AU - Gill, P

AU - d'Aloja, E

AU - Dupuy, B

AU - Eriksen, Birthe

AU - Jangblad, M

AU - Johnsson, V

AU - Kloosterman, A D

AU - Kratzer, A

AU - Lareu, M V

AU - Mevag, B

AU - Morling, N

AU - Phillips, C

AU - Pfitzinger, H

AU - Rand, S

AU - Sabatier, M

AU - Scheithauer, R

AU - Schmitter, H

AU - Schneider, Peter M.

AU - Skitsa, I

AU - Vide, M C

N1 - Keywords: Alleles; DNA Fingerprinting; DNA, Satellite; Europe; Humans; Immunoglobulin Variable Region; International Cooperation; Minisatellite Repeats; Polymerase Chain Reaction; Reproducibility of Results; Societies, Medical

PY - 1998

Y1 - 1998

N2 - This paper describes the results of three collaborative exercises which continues the EDNAP theme to explore whether uniformity of DNA profiling results could be achieved between European laboratories using STRs. In an earlier exercise, complex hypervariable AAAG-repeat STR loci were investigated, but reproducibility was found to be poor because of the variation of techniques used by participating laboratories. In the exercise reported here, an internal allelic ladder composed of ACTBP2 and D11S554 fragments was distributed. This ladder was used to size ACTBP2 analysed by a "singleplex" PCR amplification and D11S554 combined with APOAI1 in a separate "duplex" reaction. Laboratories were asked to test 7 blood stains, one of which was a known control, and to report the results to the co-ordinating laboratory. The exercise demonstrated that ACTBP2 showed good reproducibility between laboratories, whereas further testing would be needed to validate APOAI1 and D11S554 for interlaboratory comparisons. In separate exercises, the simple loci D12S391 and D1S1656 were tested; both of these showed excellent reproducibility between laboratories.

AB - This paper describes the results of three collaborative exercises which continues the EDNAP theme to explore whether uniformity of DNA profiling results could be achieved between European laboratories using STRs. In an earlier exercise, complex hypervariable AAAG-repeat STR loci were investigated, but reproducibility was found to be poor because of the variation of techniques used by participating laboratories. In the exercise reported here, an internal allelic ladder composed of ACTBP2 and D11S554 fragments was distributed. This ladder was used to size ACTBP2 analysed by a "singleplex" PCR amplification and D11S554 combined with APOAI1 in a separate "duplex" reaction. Laboratories were asked to test 7 blood stains, one of which was a known control, and to report the results to the co-ordinating laboratory. The exercise demonstrated that ACTBP2 showed good reproducibility between laboratories, whereas further testing would be needed to validate APOAI1 and D11S554 for interlaboratory comparisons. In separate exercises, the simple loci D12S391 and D1S1656 were tested; both of these showed excellent reproducibility between laboratories.

M3 - Journal article

C2 - 9924788

SN - 0379-0738

VL - 98

SP - 193

EP - 200

JO - Forensic Science International

JF - Forensic Science International

IS - 3

ER -