Relationships between human vitality and mitochondrial respiratory parameters, reactive oxygen species production and dNTP levels in peripheral blood mononuclear cells

Scott Maynard; Guido Keijzers; Martin Gram; Claus Desler Madsen; Laila Bendix; Esben Budtz-Jørgensen; Drude Molbo; Deborah L Croteau; Merete Osler; Tinna V. Stevnsner; Lene Juel Rasmussen; Flemming Dela; Kirsten Avlund; Vilhelm A Bohr

Relationships between human vitality and mitochondrial respiratory parameters, reactive oxygen species production and dNTP levels in peripheral blood mononuclear cells

Scott Maynard, Guido Keijzers, Martin Gram, Claus Desler Madsen, Laila Bendix, Esben Budtz-Jørgensen, Drude Molbo, Deborah L Croteau, Merete Osler, Tinna V. Stevnsner, Lene Juel Rasmussen, Flemming Dela, Kirsten Avlund, Vilhelm A Bohr

27 Citations (Scopus)

Abstract

Low vitality (a component of fatigue) in middle-aged and older adults is an important complaint often identified as a symptom of a disease state or side effect of a treatment. No studies to date have investigated the potential link between dysfunctional mitochondrial ATP production and low vitality. Therefore, we measured a number of cellular parameters related to mitochondrial activity in peripheral blood mononuclear cells (PBMCs) isolated from middle-aged men, and tested for association with vitality. These parameters estimate mitochondrial respiration, reactive oxygen species (ROS) production, and deoxyribonucleotide (dNTP) balance in PBMCs. The population was drawn from the Metropolit cohort of men born in 1953. Vitality level was estimated from the Medical Outcomes Study Short Form 36 (SF-36) vitality scale. We found that vitality score had no association with any of the mitochondrial respiration parameters. However, vitality score was inversely associated with cellular ROS production and cellular deoxythymidine triphosphate (dTTP) levels and positively associated with deoxycytidine triphosphate (dCTP) levels. We conclude that self-reported persistent low vitality is not associated with specific aspects of mitochondrial oxidative phosphorylation capacity in PBMCs, but may have other underlying cellular dysfunctions that contribute to dNTP imbalance and altered ROS production.

Original language	English
Journal	Aging
Pages (from-to)	850-864
Number of pages	15
ISSN	1945-4589
Publication status	Published - 30 Nov 2013

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Maynard, S., Keijzers, G., Gram, M., Madsen, C. D., Bendix, L., Budtz-Jørgensen, E., Molbo, D., Croteau, D. L., Osler, M., Stevnsner, T. V., Rasmussen, L. J., Dela, F., Avlund, K., & Bohr, V. A. (2013). Relationships between human vitality and mitochondrial respiratory parameters, reactive oxygen species production and dNTP levels in peripheral blood mononuclear cells. Aging, 850-864. http://www.impactaging.com/papers/v5/n11/full/100618.html

Maynard, S, Keijzers, G, Gram, M, Madsen, CD, Bendix, L, Budtz-Jørgensen, E , Molbo, D, Croteau, DL, Osler, M, Stevnsner, TV, Rasmussen, LJ , Dela, F, Avlund, K & Bohr, VA 2013, 'Relationships between human vitality and mitochondrial respiratory parameters, reactive oxygen species production and dNTP levels in peripheral blood mononuclear cells', Aging, pp. 850-864. <http://www.impactaging.com/papers/v5/n11/full/100618.html>

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title = "Relationships between human vitality and mitochondrial respiratory parameters, reactive oxygen species production and dNTP levels in peripheral blood mononuclear cells",

abstract = "Low vitality (a component of fatigue) in middle-aged and older adults is an important complaint often identified as a symptom of a disease state or side effect of a treatment. No studies to date have investigated the potential link between dysfunctional mitochondrial ATP production and low vitality. Therefore, we measured a number of cellular parameters related to mitochondrial activity in peripheral blood mononuclear cells (PBMCs) isolated from middle-aged men, and tested for association with vitality. These parameters estimate mitochondrial respiration, reactive oxygen species (ROS) production, and deoxyribonucleotide (dNTP) balance in PBMCs. The population was drawn from the Metropolit cohort of men born in 1953. Vitality level was estimated from the Medical Outcomes Study Short Form 36 (SF-36) vitality scale. We found that vitality score had no association with any of the mitochondrial respiration parameters. However, vitality score was inversely associated with cellular ROS production and cellular deoxythymidine triphosphate (dTTP) levels and positively associated with deoxycytidine triphosphate (dCTP) levels. We conclude that self-reported persistent low vitality is not associated with specific aspects of mitochondrial oxidative phosphorylation capacity in PBMCs, but may have other underlying cellular dysfunctions that contribute to dNTP imbalance and altered ROS production.",

author = "Scott Maynard and Guido Keijzers and Martin Gram and Madsen, {Claus Desler} and Laila Bendix and Esben Budtz-J{\o}rgensen and Drude Molbo and Croteau, {Deborah L} and Merete Osler and Stevnsner, {Tinna V.} and Rasmussen, {Lene Juel} and Flemming Dela and Kirsten Avlund and Bohr, {Vilhelm A}",

year = "2013",

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day = "30",

language = "English",

pages = "850--864",

journal = "Aging",

issn = "1945-4589",

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TY - JOUR

T1 - Relationships between human vitality and mitochondrial respiratory parameters, reactive oxygen species production and dNTP levels in peripheral blood mononuclear cells

AU - Maynard, Scott

AU - Keijzers, Guido

AU - Gram, Martin

AU - Madsen, Claus Desler

AU - Bendix, Laila

AU - Budtz-Jørgensen, Esben

AU - Molbo, Drude

AU - Croteau, Deborah L

AU - Osler, Merete

AU - Stevnsner, Tinna V.

AU - Rasmussen, Lene Juel

AU - Dela, Flemming

AU - Avlund, Kirsten

AU - Bohr, Vilhelm A

PY - 2013/11/30

Y1 - 2013/11/30

N2 - Low vitality (a component of fatigue) in middle-aged and older adults is an important complaint often identified as a symptom of a disease state or side effect of a treatment. No studies to date have investigated the potential link between dysfunctional mitochondrial ATP production and low vitality. Therefore, we measured a number of cellular parameters related to mitochondrial activity in peripheral blood mononuclear cells (PBMCs) isolated from middle-aged men, and tested for association with vitality. These parameters estimate mitochondrial respiration, reactive oxygen species (ROS) production, and deoxyribonucleotide (dNTP) balance in PBMCs. The population was drawn from the Metropolit cohort of men born in 1953. Vitality level was estimated from the Medical Outcomes Study Short Form 36 (SF-36) vitality scale. We found that vitality score had no association with any of the mitochondrial respiration parameters. However, vitality score was inversely associated with cellular ROS production and cellular deoxythymidine triphosphate (dTTP) levels and positively associated with deoxycytidine triphosphate (dCTP) levels. We conclude that self-reported persistent low vitality is not associated with specific aspects of mitochondrial oxidative phosphorylation capacity in PBMCs, but may have other underlying cellular dysfunctions that contribute to dNTP imbalance and altered ROS production.

AB - Low vitality (a component of fatigue) in middle-aged and older adults is an important complaint often identified as a symptom of a disease state or side effect of a treatment. No studies to date have investigated the potential link between dysfunctional mitochondrial ATP production and low vitality. Therefore, we measured a number of cellular parameters related to mitochondrial activity in peripheral blood mononuclear cells (PBMCs) isolated from middle-aged men, and tested for association with vitality. These parameters estimate mitochondrial respiration, reactive oxygen species (ROS) production, and deoxyribonucleotide (dNTP) balance in PBMCs. The population was drawn from the Metropolit cohort of men born in 1953. Vitality level was estimated from the Medical Outcomes Study Short Form 36 (SF-36) vitality scale. We found that vitality score had no association with any of the mitochondrial respiration parameters. However, vitality score was inversely associated with cellular ROS production and cellular deoxythymidine triphosphate (dTTP) levels and positively associated with deoxycytidine triphosphate (dCTP) levels. We conclude that self-reported persistent low vitality is not associated with specific aspects of mitochondrial oxidative phosphorylation capacity in PBMCs, but may have other underlying cellular dysfunctions that contribute to dNTP imbalance and altered ROS production.

M3 - Journal article

C2 - 24304678

SN - 1945-4589

SP - 850

EP - 864

JO - Aging

JF - Aging

ER -

Relationships between human vitality and mitochondrial respiratory parameters, reactive oxygen species production and dNTP levels in peripheral blood mononuclear cells

Abstract

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