Regulation of MIR165/166 by class II and class III homeodomain leucine zipper proteins establishes leaf polarity

Paz Merelo; Hathi Ram; Monica Pia Caggiano; Carolyn Ohno; Felix Ott; Daniel Straub; Moritz Graeff; Seok Keun Cho; Seong Wook Yang; Stephan Wenkel; Marcus G. Heisler

doi:10.1073/pnas.1516110113

Regulation of MIR165/166 by class II and class III homeodomain leucine zipper proteins establishes leaf polarity

Paz Merelo, Hathi Ram, Monica Pia Caggiano, Carolyn Ohno, Felix Ott, Daniel Straub, Moritz Graeff, Seok Keun Cho, Seong Wook Yang, Stephan Wenkel, Marcus G. Heisler

49 Citations (Scopus)

Abstract

A defining feature of plant leaves is their flattened shape. This shape depends on an antagonism between the genes that specify adaxial (top) and abaxial (bottom) tissue identity; however, the molecular nature of this antagonism remains poorly understood. Class III homeodomain leucine zipper (HD-ZIP) transcription factors are key mediators in the regulation of adaxial-abaxial patterning. Their expression is restricted adaxially during early development by the abaxially expressed microRNA (MIR)165/166, yet the mechanism that restricts MIR165/166 expression to abaxial leaf tissues remains unknown. Here, we show that class III and class II HD-ZIP proteins act together to repress MIR165/166 via a conserved cis-element in their promoters. Organ morphology and tissue patterning in plants, therefore, depend on a bidirectional repressive circuit involving a set of miRNAs and its targets.

Original language	English
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	113
Issue number	42
Pages (from-to)	11973-11978
Number of pages	6
ISSN	0027-8424
DOIs	https://doi.org/10.1073/pnas.1516110113
Publication status	Published - 18 Oct 2016

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Merelo, P., Ram, H., Caggiano, M. P., Ohno, C., Ott, F., Straub, D., Graeff, M., Cho, S. K., Yang, S. W., Wenkel, S., & Heisler, M. G. (2016). Regulation of MIR165/166 by class II and class III homeodomain leucine zipper proteins establishes leaf polarity. Proceedings of the National Academy of Sciences of the United States of America, 113(42), 11973-11978. https://doi.org/10.1073/pnas.1516110113

Regulation of MIR165/166 by class II and class III homeodomain leucine zipper proteins establishes leaf polarity. / Merelo, Paz; Ram, Hathi; Caggiano, Monica Pia et al.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 113, No. 42, 18.10.2016, p. 11973-11978.

Research output: Contribution to journal › Journal article › Research › peer-review

Merelo, P, Ram, H, Caggiano, MP, Ohno, C, Ott, F, Straub, D, Graeff, M, Cho, SK, Yang, SW, Wenkel, S & Heisler, MG 2016, 'Regulation of MIR165/166 by class II and class III homeodomain leucine zipper proteins establishes leaf polarity', Proceedings of the National Academy of Sciences of the United States of America, vol. 113, no. 42, pp. 11973-11978. https://doi.org/10.1073/pnas.1516110113

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title = "Regulation of MIR165/166 by class II and class III homeodomain leucine zipper proteins establishes leaf polarity",

abstract = "A defining feature of plant leaves is their flattened shape. This shape depends on an antagonism between the genes that specify adaxial (top) and abaxial (bottom) tissue identity; however, the molecular nature of this antagonism remains poorly understood. Class III homeodomain leucine zipper (HD-ZIP) transcription factors are key mediators in the regulation of adaxial-abaxial patterning. Their expression is restricted adaxially during early development by the abaxially expressed microRNA (MIR)165/166, yet the mechanism that restricts MIR165/166 expression to abaxial leaf tissues remains unknown. Here, we show that class III and class II HD-ZIP proteins act together to repress MIR165/166 via a conserved cis-element in their promoters. Organ morphology and tissue patterning in plants, therefore, depend on a bidirectional repressive circuit involving a set of miRNAs and its targets.",

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AU - Merelo, Paz

AU - Ram, Hathi

AU - Caggiano, Monica Pia

AU - Ohno, Carolyn

AU - Ott, Felix

AU - Straub, Daniel

AU - Graeff, Moritz

AU - Cho, Seok Keun

AU - Yang, Seong Wook

AU - Wenkel, Stephan

AU - Heisler, Marcus G.

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AB - A defining feature of plant leaves is their flattened shape. This shape depends on an antagonism between the genes that specify adaxial (top) and abaxial (bottom) tissue identity; however, the molecular nature of this antagonism remains poorly understood. Class III homeodomain leucine zipper (HD-ZIP) transcription factors are key mediators in the regulation of adaxial-abaxial patterning. Their expression is restricted adaxially during early development by the abaxially expressed microRNA (MIR)165/166, yet the mechanism that restricts MIR165/166 expression to abaxial leaf tissues remains unknown. Here, we show that class III and class II HD-ZIP proteins act together to repress MIR165/166 via a conserved cis-element in their promoters. Organ morphology and tissue patterning in plants, therefore, depend on a bidirectional repressive circuit involving a set of miRNAs and its targets.

U2 - 10.1073/pnas.1516110113

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JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

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ER -

Regulation of MIR165/166 by class II and class III homeodomain leucine zipper proteins establishes leaf polarity

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