Regulation of MIR165/166 by class II and class III homeodomain leucine zipper proteins establishes leaf polarity

TY - JOUR

T1 - Regulation of MIR165/166 by class II and class III homeodomain leucine zipper proteins establishes leaf polarity

AU - Merelo, Paz

AU - Ram, Hathi

AU - Caggiano, Monica Pia

AU - Ohno, Carolyn

AU - Ott, Felix

AU - Straub, Daniel

AU - Graeff, Moritz

AU - Cho, Seok Keun

AU - Yang, Seong Wook

AU - Wenkel, Stephan

AU - Heisler, Marcus G.

PY - 2016/10/18

Y1 - 2016/10/18

N2 - A defining feature of plant leaves is their flattened shape. This shape depends on an antagonism between the genes that specify adaxial (top) and abaxial (bottom) tissue identity; however, the molecular nature of this antagonism remains poorly understood. Class III homeodomain leucine zipper (HD-ZIP) transcription factors are key mediators in the regulation of adaxial-abaxial patterning. Their expression is restricted adaxially during early development by the abaxially expressed microRNA (MIR)165/166, yet the mechanism that restricts MIR165/166 expression to abaxial leaf tissues remains unknown. Here, we show that class III and class II HD-ZIP proteins act together to repress MIR165/166 via a conserved cis-element in their promoters. Organ morphology and tissue patterning in plants, therefore, depend on a bidirectional repressive circuit involving a set of miRNAs and its targets.

AB - A defining feature of plant leaves is their flattened shape. This shape depends on an antagonism between the genes that specify adaxial (top) and abaxial (bottom) tissue identity; however, the molecular nature of this antagonism remains poorly understood. Class III homeodomain leucine zipper (HD-ZIP) transcription factors are key mediators in the regulation of adaxial-abaxial patterning. Their expression is restricted adaxially during early development by the abaxially expressed microRNA (MIR)165/166, yet the mechanism that restricts MIR165/166 expression to abaxial leaf tissues remains unknown. Here, we show that class III and class II HD-ZIP proteins act together to repress MIR165/166 via a conserved cis-element in their promoters. Organ morphology and tissue patterning in plants, therefore, depend on a bidirectional repressive circuit involving a set of miRNAs and its targets.

U2 - 10.1073/pnas.1516110113

DO - 10.1073/pnas.1516110113

M3 - Journal article

C2 - 27698117

SN - 0027-8424

VL - 113

SP - 11973

EP - 11978

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 42

ER -