Abstract
The suitable optical properties of thiol-ene polymers combined with the ease of modifying their surface for the attachment of recognition molecules make them ideal candidates in many biochip applications. This paper reports the rapid one-step photochemical surface patterning of biomolecules in microfluidic thiol-ene chips. This work focuses on thiol-ene substrates featuring an excess of thiol groups at their surface. The thiol-ene stoichiometric composition can be varied to precisely control the number of surface thiol groups available for surface modification up to an average surface density of 136 ± 17 SH nm(-2). Biotin alkyne was patterned directly inside thiol-ene microchannels prior to conjugation with fluorescently labelled streptavidin. The surface bound conjugates were detected by evanescent wave-induced fluorescence (EWIF), demonstrating the success of the grafting procedure and its potential for biochip applications.
| Original language | English |
|---|---|
| Journal | Analyst |
| Volume | 138 |
| Issue number | 3 |
| Pages (from-to) | 845-849 |
| Number of pages | 5 |
| ISSN | 0003-2654 |
| DOIs | |
| Publication status | Published - 7 Feb 2013 |
| Externally published | Yes |
Keywords
- Alkynes
- Biotin
- Fluorescent Dyes
- Microfluidic Analytical Techniques
- Polymers
- Spectrometry, Fluorescence
- Streptavidin
- Sulfhydryl Compounds
- Surface Properties