Rapid photochemical surface patterning of proteins in thiol-ene based microfluidic devices

TY - JOUR

T1 - Rapid photochemical surface patterning of proteins in thiol-ene based microfluidic devices

AU - Lafleur, Josiane P.

AU - Jensen, T.G.

AU - Kutter, Jörg P.

AU - Kwapiszewski, R.

PY - 2013/2/7

Y1 - 2013/2/7

N2 - The suitable optical properties of thiol-ene polymers combined with the ease of modifying their surface for the attachment of recognition molecules make them ideal candidates in many biochip applications. This paper reports the rapid one-step photochemical surface patterning of biomolecules in microfluidic thiol-ene chips. This work focuses on thiol-ene substrates featuring an excess of thiol groups at their surface. The thiol-ene stoichiometric composition can be varied to precisely control the number of surface thiol groups available for surface modification up to an average surface density of 136 ± 17 SH nm(-2). Biotin alkyne was patterned directly inside thiol-ene microchannels prior to conjugation with fluorescently labelled streptavidin. The surface bound conjugates were detected by evanescent wave-induced fluorescence (EWIF), demonstrating the success of the grafting procedure and its potential for biochip applications.

AB - The suitable optical properties of thiol-ene polymers combined with the ease of modifying their surface for the attachment of recognition molecules make them ideal candidates in many biochip applications. This paper reports the rapid one-step photochemical surface patterning of biomolecules in microfluidic thiol-ene chips. This work focuses on thiol-ene substrates featuring an excess of thiol groups at their surface. The thiol-ene stoichiometric composition can be varied to precisely control the number of surface thiol groups available for surface modification up to an average surface density of 136 ± 17 SH nm(-2). Biotin alkyne was patterned directly inside thiol-ene microchannels prior to conjugation with fluorescently labelled streptavidin. The surface bound conjugates were detected by evanescent wave-induced fluorescence (EWIF), demonstrating the success of the grafting procedure and its potential for biochip applications.

KW - Alkynes

KW - Biotin

KW - Fluorescent Dyes

KW - Microfluidic Analytical Techniques

KW - Polymers

KW - Spectrometry, Fluorescence

KW - Streptavidin

KW - Sulfhydryl Compounds

KW - Surface Properties

UR - http://www.scopus.com/inward/record.url?scp=84872092079&partnerID=8YFLogxK

U2 - 10.1039/c2an36424g

DO - 10.1039/c2an36424g

M3 - Journal article

C2 - 23193537

AN - SCOPUS:84872092079

SN - 0003-2654

VL - 138

SP - 845

EP - 849

JO - Analyst

JF - Analyst

IS - 3

ER -