Rapid photochemical surface patterning of proteins in thiol-ene based microfluidic devices

Josiane P. Lafleur; T.G. Jensen; Jörg P. Kutter; R. Kwapiszewski

doi:10.1039/c2an36424g

Rapid photochemical surface patterning of proteins in thiol-ene based microfluidic devices

Josiane P. Lafleur, T.G. Jensen, Jörg P. Kutter, R. Kwapiszewski

46 Citationer (Scopus)

Abstract

The suitable optical properties of thiol-ene polymers combined with the ease of modifying their surface for the attachment of recognition molecules make them ideal candidates in many biochip applications. This paper reports the rapid one-step photochemical surface patterning of biomolecules in microfluidic thiol-ene chips. This work focuses on thiol-ene substrates featuring an excess of thiol groups at their surface. The thiol-ene stoichiometric composition can be varied to precisely control the number of surface thiol groups available for surface modification up to an average surface density of 136 ± 17 SH nm(-2). Biotin alkyne was patterned directly inside thiol-ene microchannels prior to conjugation with fluorescently labelled streptavidin. The surface bound conjugates were detected by evanescent wave-induced fluorescence (EWIF), demonstrating the success of the grafting procedure and its potential for biochip applications.

Originalsprog	Engelsk
Tidsskrift	Analyst
Vol/bind	138
Udgave nummer	3
Sider (fra-til)	845-849
Antal sider	5
ISSN	0003-2654
DOI	https://doi.org/10.1039/c2an36424g
Status	Udgivet - 7 feb. 2013
Udgivet eksternt	Ja

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title = "Rapid photochemical surface patterning of proteins in thiol-ene based microfluidic devices",

abstract = "The suitable optical properties of thiol-ene polymers combined with the ease of modifying their surface for the attachment of recognition molecules make them ideal candidates in many biochip applications. This paper reports the rapid one-step photochemical surface patterning of biomolecules in microfluidic thiol-ene chips. This work focuses on thiol-ene substrates featuring an excess of thiol groups at their surface. The thiol-ene stoichiometric composition can be varied to precisely control the number of surface thiol groups available for surface modification up to an average surface density of 136 ± 17 SH nm(-2). Biotin alkyne was patterned directly inside thiol-ene microchannels prior to conjugation with fluorescently labelled streptavidin. The surface bound conjugates were detected by evanescent wave-induced fluorescence (EWIF), demonstrating the success of the grafting procedure and its potential for biochip applications.",

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AU - Lafleur, Josiane P.

AU - Jensen, T.G.

AU - Kutter, Jörg P.

AU - Kwapiszewski, R.

PY - 2013/2/7

Y1 - 2013/2/7

N2 - The suitable optical properties of thiol-ene polymers combined with the ease of modifying their surface for the attachment of recognition molecules make them ideal candidates in many biochip applications. This paper reports the rapid one-step photochemical surface patterning of biomolecules in microfluidic thiol-ene chips. This work focuses on thiol-ene substrates featuring an excess of thiol groups at their surface. The thiol-ene stoichiometric composition can be varied to precisely control the number of surface thiol groups available for surface modification up to an average surface density of 136 ± 17 SH nm(-2). Biotin alkyne was patterned directly inside thiol-ene microchannels prior to conjugation with fluorescently labelled streptavidin. The surface bound conjugates were detected by evanescent wave-induced fluorescence (EWIF), demonstrating the success of the grafting procedure and its potential for biochip applications.

AB - The suitable optical properties of thiol-ene polymers combined with the ease of modifying their surface for the attachment of recognition molecules make them ideal candidates in many biochip applications. This paper reports the rapid one-step photochemical surface patterning of biomolecules in microfluidic thiol-ene chips. This work focuses on thiol-ene substrates featuring an excess of thiol groups at their surface. The thiol-ene stoichiometric composition can be varied to precisely control the number of surface thiol groups available for surface modification up to an average surface density of 136 ± 17 SH nm(-2). Biotin alkyne was patterned directly inside thiol-ene microchannels prior to conjugation with fluorescently labelled streptavidin. The surface bound conjugates were detected by evanescent wave-induced fluorescence (EWIF), demonstrating the success of the grafting procedure and its potential for biochip applications.

KW - Alkynes

KW - Biotin

KW - Fluorescent Dyes

KW - Microfluidic Analytical Techniques

KW - Polymers

KW - Spectrometry, Fluorescence

KW - Streptavidin

KW - Sulfhydryl Compounds

KW - Surface Properties

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JO - The Analyst

JF - The Analyst

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ER -

Rapid photochemical surface patterning of proteins in thiol-ene based microfluidic devices

Abstract

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