Rapid identification of Stenotrophomonas maltophilia by peptide nucleic acid fluorescence in situ hybridization

Nanna Reumert Knudsen; A. K. I. Rasmussen; M. J. Fiandaca; Kasper Nørskov Kragh; Thomas Bjarnsholt; Niels Høiby; H. Stender; Luca Guardabassi

doi:10.1002/nmi2.38

Rapid identification of Stenotrophomonas maltophilia by peptide nucleic acid fluorescence in situ hybridization

Nanna Reumert Knudsen, A. K. I. Rasmussen, M. J. Fiandaca, Kasper Nørskov Kragh, Thomas Bjarnsholt, Niels Høiby, H. Stender, Luca Guardabassi

5 Citations (Scopus)

Abstract

The objective of this study was to develop a novel peptide nucleic acid (PNA) probe for Stenotrophomonas maltophilia identification by fluorescence in situ hybridization (FISH). The probe was evaluated using 33 human and veterinary clinical S. maltophilia isolates and 45 reference strains representing common bacterial species in the respiratory tract. The probe displayed 100% sensitivity and 100% specificity on pure cultures and allowed detection in sputum from cystic fibrosis patients. The detection limit was 10⁴ CFU/mL in spiked tracheal aspirate and bronchoalveolar lavage from healthy horses. Altogether the study shows that this species-specific PNA FISH probe facilitates rapid detection of S. maltophilia in biological specimens.

Original language	English
Journal	New Microbes and New Infections
Volume	2
Issue number	3
Pages (from-to)	79-81
Number of pages	3
DOIs	https://doi.org/10.1002/nmi2.38
Publication status	Published - May 2014

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title = "Rapid identification of Stenotrophomonas maltophilia by peptide nucleic acid fluorescence in situ hybridization",

abstract = "The objective of this study was to develop a novel peptide nucleic acid (PNA) probe for Stenotrophomonas maltophilia identification by fluorescence in situ hybridization (FISH). The probe was evaluated using 33 human and veterinary clinical S. maltophilia isolates and 45 reference strains representing common bacterial species in the respiratory tract. The probe displayed 100% sensitivity and 100% specificity on pure cultures and allowed detection in sputum from cystic fibrosis patients. The detection limit was 104 CFU/mL in spiked tracheal aspirate and bronchoalveolar lavage from healthy horses. Altogether the study shows that this species-specific PNA FISH probe facilitates rapid detection of S. maltophilia in biological specimens.",

author = "Knudsen, {Nanna Reumert} and Rasmussen, {A. K. I.} and Fiandaca, {M. J.} and Kragh, {Kasper N{\o}rskov} and Thomas Bjarnsholt and Niels H{\o}iby and H. Stender and Luca Guardabassi",

note = "Nanna Reumert Knudsen er identisk med Nanna Hansen",

year = "2014",

month = may,

doi = "10.1002/nmi2.38",

language = "English",

volume = "2",

pages = "79--81",

journal = "New Microbes and New Infections",

issn = "2052-2975",

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TY - JOUR

T1 - Rapid identification of Stenotrophomonas maltophilia by peptide nucleic acid fluorescence in situ hybridization

AU - Knudsen, Nanna Reumert

AU - Rasmussen, A. K. I.

AU - Fiandaca, M. J.

AU - Kragh, Kasper Nørskov

AU - Bjarnsholt, Thomas

AU - Høiby, Niels

AU - Stender, H.

AU - Guardabassi, Luca

N1 - Nanna Reumert Knudsen er identisk med Nanna Hansen

PY - 2014/5

Y1 - 2014/5

N2 - The objective of this study was to develop a novel peptide nucleic acid (PNA) probe for Stenotrophomonas maltophilia identification by fluorescence in situ hybridization (FISH). The probe was evaluated using 33 human and veterinary clinical S. maltophilia isolates and 45 reference strains representing common bacterial species in the respiratory tract. The probe displayed 100% sensitivity and 100% specificity on pure cultures and allowed detection in sputum from cystic fibrosis patients. The detection limit was 104 CFU/mL in spiked tracheal aspirate and bronchoalveolar lavage from healthy horses. Altogether the study shows that this species-specific PNA FISH probe facilitates rapid detection of S. maltophilia in biological specimens.

AB - The objective of this study was to develop a novel peptide nucleic acid (PNA) probe for Stenotrophomonas maltophilia identification by fluorescence in situ hybridization (FISH). The probe was evaluated using 33 human and veterinary clinical S. maltophilia isolates and 45 reference strains representing common bacterial species in the respiratory tract. The probe displayed 100% sensitivity and 100% specificity on pure cultures and allowed detection in sputum from cystic fibrosis patients. The detection limit was 104 CFU/mL in spiked tracheal aspirate and bronchoalveolar lavage from healthy horses. Altogether the study shows that this species-specific PNA FISH probe facilitates rapid detection of S. maltophilia in biological specimens.

U2 - 10.1002/nmi2.38

DO - 10.1002/nmi2.38

M3 - Journal article

C2 - 25356348

SN - 2052-2975

VL - 2

SP - 79

EP - 81

JO - New Microbes and New Infections

JF - New Microbes and New Infections

IS - 3

ER -

Rapid identification of Stenotrophomonas maltophilia by peptide nucleic acid fluorescence in situ hybridization

Abstract

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