Rainbow trout (Oncorhynchus mykiss) immune response towards a recombinant vaccine targeting the parasitic ciliate Ichthyophthirius multifiliis

Louise von Gersdorff Jørgensen; Per Walter Kania; Karina Juhl Rasmussen; Andreas  Mattsson; Jacob Günther Schmidt; Azmi Al-Jubury; Adam Frederik Sander Pedersen; Ali Salanti; Kurt Buchmann

doi:10.1111/jfd.12653

Rainbow trout (Oncorhynchus mykiss) immune response towards a recombinant vaccine targeting the parasitic ciliate Ichthyophthirius multifiliis

Louise von Gersdorff Jørgensen, Per Walter Kania, Karina Juhl Rasmussen, Andreas Mattsson, Jacob Günther Schmidt, Azmi Al-Jubury, Adam Frederik Sander Pedersen, Ali Salanti, Kurt Buchmann

10 Citations (Scopus)

Abstract

The protective effect in rainbow trout (Oncorhynchus mykiss) of an experimental subunit vaccine targeting antigens in the parasite Ichthyophthirius multifiliis has been evaluated and compared to effects elicited by a classical parasite homogenate vaccine. Three recombinant parasite proteins (two produced in E. coli and one in insect cells) were combined and injected i.p., and subsequently, protection and antibody responses were analysed. Both the experimental and the benchmark vaccine induced partial but significant protection against I. multifiliis when compared to control fish. Specific antibody responses of vaccinated trout (subunit vaccine) were raised against one neurohypophysial n-terminal domain protein #10 of three recombinant proteins, whereas the benchmark vaccine group showed specific antibody production against all three recombinant proteins. The immunogenic parasite protein #10 may be a potential vaccine candidate supplementing the protective I-antigen in future vaccine trials.

Original language	English
Journal	Journal of Fish Diseases
Volume	40
Issue number	12
Pages (from-to)	1815–1821
ISSN	0140-7775
DOIs	https://doi.org/10.1111/jfd.12653
Publication status	Published - Dec 2017

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Jørgensen, L. V. G., Kania, P. W., Rasmussen, K. J., Mattsson, A., Schmidt, J. G., Al-Jubury, A., Pedersen, A. F. S., Salanti, A., & Buchmann, K. (2017). Rainbow trout (Oncorhynchus mykiss) immune response towards a recombinant vaccine targeting the parasitic ciliate Ichthyophthirius multifiliis. Journal of Fish Diseases, 40(12), 1815–1821. https://doi.org/10.1111/jfd.12653

Rainbow trout (Oncorhynchus mykiss) immune response towards a recombinant vaccine targeting the parasitic ciliate Ichthyophthirius multifiliis. / Jørgensen, Louise von Gersdorff ; Kania, Per Walter; Rasmussen, Karina Juhl et al.
In: Journal of Fish Diseases, Vol. 40, No. 12, 12.2017, p. 1815–1821.

Research output: Contribution to journal › Journal article › Research › peer-review

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abstract = "The protective effect in rainbow trout (Oncorhynchus mykiss) of an experimental subunit vaccine targeting antigens in the parasite Ichthyophthirius multifiliis has been evaluated and compared to effects elicited by a classical parasite homogenate vaccine. Three recombinant parasite proteins (two produced in E. coli and one in insect cells) were combined and injected i.p., and subsequently, protection and antibody responses were analysed. Both the experimental and the benchmark vaccine induced partial but significant protection against I. multifiliis when compared to control fish. Specific antibody responses of vaccinated trout (subunit vaccine) were raised against one neurohypophysial n-terminal domain protein #10 of three recombinant proteins, whereas the benchmark vaccine group showed specific antibody production against all three recombinant proteins. The immunogenic parasite protein #10 may be a potential vaccine candidate supplementing the protective I-antigen in future vaccine trials.",

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AU - Jørgensen, Louise von Gersdorff

AU - Kania, Per Walter

AU - Rasmussen, Karina Juhl

AU - Mattsson, Andreas

AU - Schmidt, Jacob Günther

AU - Al-Jubury, Azmi

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AU - Salanti, Ali

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AB - The protective effect in rainbow trout (Oncorhynchus mykiss) of an experimental subunit vaccine targeting antigens in the parasite Ichthyophthirius multifiliis has been evaluated and compared to effects elicited by a classical parasite homogenate vaccine. Three recombinant parasite proteins (two produced in E. coli and one in insect cells) were combined and injected i.p., and subsequently, protection and antibody responses were analysed. Both the experimental and the benchmark vaccine induced partial but significant protection against I. multifiliis when compared to control fish. Specific antibody responses of vaccinated trout (subunit vaccine) were raised against one neurohypophysial n-terminal domain protein #10 of three recombinant proteins, whereas the benchmark vaccine group showed specific antibody production against all three recombinant proteins. The immunogenic parasite protein #10 may be a potential vaccine candidate supplementing the protective I-antigen in future vaccine trials.

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Rainbow trout (Oncorhynchus mykiss) immune response towards a recombinant vaccine targeting the parasitic ciliate Ichthyophthirius multifiliis

Abstract

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