Quorum sensing regulation in Aeromonas hydrophila

Christian Garde; Thomas Bjarnsholt; Michael Givskov; Tim Holm Jakobsen; Morten Hentzer; Anetta Claussen; Kim Sneppen; Jesper Ferkinghoff-Borg; Thomas Sams

doi:10.1016/j.jmb.2010.01.002

Quorum sensing regulation in Aeromonas hydrophila

Christian Garde, Thomas Bjarnsholt, Michael Givskov, Tim Holm Jakobsen, Morten Hentzer, Anetta Claussen, Kim Sneppen, Jesper Ferkinghoff-Borg, Thomas Sams

Department of Immunology and Microbiology

33 Citations (Scopus)

Abstract

We present detailed results on the C4-HSL-mediated quorum sensing (QS) regulatory system of the opportunistic Gram-negative bacterium Aeromonas hydrophila. This bacterium contains a particularly simple QS system that allows for a detailed modeling of kinetics. In a model system (i.e., the Escherichia coli monitor strain MH205), the C4-HSL production of A. hydrophila is interrupted by fusion of gfp(ASV). In the present in vitro study, we measure the response of the QS regulatory ahyRI locus in the monitor strain to predetermined concentrations of C4-HSL signal molecules. A minimal kinetic model describes the data well. It can be solved analytically, providing substantial insight into the QS mechanism: at high concentrations of signal molecules, a slow decay of the activated regulator sets the timescale for the QS regulation loop. Slow saturation ensures that, in an A. hydrophila cell, the QS system is activated only by signal molecules produced by other A. hydrophila cells. Separate information on the ahyR and ahyI loci can be extracted, thus allowing the probe to be used in identifying the target when testing QS inhibitors.

Original language	English
Journal	Journal of Molecular Biology
Volume	396
Issue number	4
Pages (from-to)	849-57
Number of pages	9
ISSN	0022-2836
DOIs	https://doi.org/10.1016/j.jmb.2010.01.002
Publication status	Published - 2010

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10.1016/j.jmb.2010.01.002

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@article{8acb6770a2e911df928f000ea68e967b,

title = "Quorum sensing regulation in Aeromonas hydrophila",

abstract = "We present detailed results on the C4-HSL-mediated quorum sensing (QS) regulatory system of the opportunistic Gram-negative bacterium Aeromonas hydrophila. This bacterium contains a particularly simple QS system that allows for a detailed modeling of kinetics. In a model system (i.e., the Escherichia coli monitor strain MH205), the C4-HSL production of A. hydrophila is interrupted by fusion of gfp(ASV). In the present in vitro study, we measure the response of the QS regulatory ahyRI locus in the monitor strain to predetermined concentrations of C4-HSL signal molecules. A minimal kinetic model describes the data well. It can be solved analytically, providing substantial insight into the QS mechanism: at high concentrations of signal molecules, a slow decay of the activated regulator sets the timescale for the QS regulation loop. Slow saturation ensures that, in an A. hydrophila cell, the QS system is activated only by signal molecules produced by other A. hydrophila cells. Separate information on the ahyR and ahyI loci can be extracted, thus allowing the probe to be used in identifying the target when testing QS inhibitors.",

author = "Christian Garde and Thomas Bjarnsholt and Michael Givskov and Jakobsen, {Tim Holm} and Morten Hentzer and Anetta Claussen and Kim Sneppen and Jesper Ferkinghoff-Borg and Thomas Sams",

note = "Keywords: 4-Butyrolactone; Aeromonas hydrophila; Amino Acid Sequence; Bacterial Proteins; Base Sequence; DNA, Bacterial; Escherichia coli; Genes, Bacterial; Green Fluorescent Proteins; Kinetics; Models, Biological; Molecular Sequence Data; Promoter Regions, Genetic; Quorum Sensing; Recombinant Proteins",

year = "2010",

doi = "10.1016/j.jmb.2010.01.002",

language = "English",

volume = "396",

pages = "849--57",

journal = "Journal of Molecular Biology",

issn = "0022-2836",

publisher = "Academic Press",

number = "4",

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TY - JOUR

T1 - Quorum sensing regulation in Aeromonas hydrophila

AU - Garde, Christian

AU - Bjarnsholt, Thomas

AU - Givskov, Michael

AU - Jakobsen, Tim Holm

AU - Hentzer, Morten

AU - Claussen, Anetta

AU - Sneppen, Kim

AU - Ferkinghoff-Borg, Jesper

AU - Sams, Thomas

N1 - Keywords: 4-Butyrolactone; Aeromonas hydrophila; Amino Acid Sequence; Bacterial Proteins; Base Sequence; DNA, Bacterial; Escherichia coli; Genes, Bacterial; Green Fluorescent Proteins; Kinetics; Models, Biological; Molecular Sequence Data; Promoter Regions, Genetic; Quorum Sensing; Recombinant Proteins

PY - 2010

Y1 - 2010

N2 - We present detailed results on the C4-HSL-mediated quorum sensing (QS) regulatory system of the opportunistic Gram-negative bacterium Aeromonas hydrophila. This bacterium contains a particularly simple QS system that allows for a detailed modeling of kinetics. In a model system (i.e., the Escherichia coli monitor strain MH205), the C4-HSL production of A. hydrophila is interrupted by fusion of gfp(ASV). In the present in vitro study, we measure the response of the QS regulatory ahyRI locus in the monitor strain to predetermined concentrations of C4-HSL signal molecules. A minimal kinetic model describes the data well. It can be solved analytically, providing substantial insight into the QS mechanism: at high concentrations of signal molecules, a slow decay of the activated regulator sets the timescale for the QS regulation loop. Slow saturation ensures that, in an A. hydrophila cell, the QS system is activated only by signal molecules produced by other A. hydrophila cells. Separate information on the ahyR and ahyI loci can be extracted, thus allowing the probe to be used in identifying the target when testing QS inhibitors.

AB - We present detailed results on the C4-HSL-mediated quorum sensing (QS) regulatory system of the opportunistic Gram-negative bacterium Aeromonas hydrophila. This bacterium contains a particularly simple QS system that allows for a detailed modeling of kinetics. In a model system (i.e., the Escherichia coli monitor strain MH205), the C4-HSL production of A. hydrophila is interrupted by fusion of gfp(ASV). In the present in vitro study, we measure the response of the QS regulatory ahyRI locus in the monitor strain to predetermined concentrations of C4-HSL signal molecules. A minimal kinetic model describes the data well. It can be solved analytically, providing substantial insight into the QS mechanism: at high concentrations of signal molecules, a slow decay of the activated regulator sets the timescale for the QS regulation loop. Slow saturation ensures that, in an A. hydrophila cell, the QS system is activated only by signal molecules produced by other A. hydrophila cells. Separate information on the ahyR and ahyI loci can be extracted, thus allowing the probe to be used in identifying the target when testing QS inhibitors.

U2 - 10.1016/j.jmb.2010.01.002

DO - 10.1016/j.jmb.2010.01.002

M3 - Journal article

C2 - 20064524

SN - 0022-2836

VL - 396

SP - 849

EP - 857

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

IS - 4

ER -

Quorum sensing regulation in Aeromonas hydrophila

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