TY - JOUR
T1 - Quorum sensing regulation in Aeromonas hydrophila
AU - Garde, Christian
AU - Bjarnsholt, Thomas
AU - Givskov, Michael
AU - Jakobsen, Tim Holm
AU - Hentzer, Morten
AU - Claussen, Anetta
AU - Sneppen, Kim
AU - Ferkinghoff-Borg, Jesper
AU - Sams, Thomas
N1 - Keywords: 4-Butyrolactone; Aeromonas hydrophila; Amino Acid Sequence; Bacterial Proteins; Base Sequence; DNA, Bacterial; Escherichia coli; Genes, Bacterial; Green Fluorescent Proteins; Kinetics; Models, Biological; Molecular Sequence Data; Promoter Regions, Genetic; Quorum Sensing; Recombinant Proteins
PY - 2010
Y1 - 2010
N2 - We present detailed results on the C4-HSL-mediated quorum sensing (QS) regulatory system of the opportunistic Gram-negative bacterium Aeromonas hydrophila. This bacterium contains a particularly simple QS system that allows for a detailed modeling of kinetics. In a model system (i.e., the Escherichia coli monitor strain MH205), the C4-HSL production of A. hydrophila is interrupted by fusion of gfp(ASV). In the present in vitro study, we measure the response of the QS regulatory ahyRI locus in the monitor strain to predetermined concentrations of C4-HSL signal molecules. A minimal kinetic model describes the data well. It can be solved analytically, providing substantial insight into the QS mechanism: at high concentrations of signal molecules, a slow decay of the activated regulator sets the timescale for the QS regulation loop. Slow saturation ensures that, in an A. hydrophila cell, the QS system is activated only by signal molecules produced by other A. hydrophila cells. Separate information on the ahyR and ahyI loci can be extracted, thus allowing the probe to be used in identifying the target when testing QS inhibitors.
AB - We present detailed results on the C4-HSL-mediated quorum sensing (QS) regulatory system of the opportunistic Gram-negative bacterium Aeromonas hydrophila. This bacterium contains a particularly simple QS system that allows for a detailed modeling of kinetics. In a model system (i.e., the Escherichia coli monitor strain MH205), the C4-HSL production of A. hydrophila is interrupted by fusion of gfp(ASV). In the present in vitro study, we measure the response of the QS regulatory ahyRI locus in the monitor strain to predetermined concentrations of C4-HSL signal molecules. A minimal kinetic model describes the data well. It can be solved analytically, providing substantial insight into the QS mechanism: at high concentrations of signal molecules, a slow decay of the activated regulator sets the timescale for the QS regulation loop. Slow saturation ensures that, in an A. hydrophila cell, the QS system is activated only by signal molecules produced by other A. hydrophila cells. Separate information on the ahyR and ahyI loci can be extracted, thus allowing the probe to be used in identifying the target when testing QS inhibitors.
U2 - 10.1016/j.jmb.2010.01.002
DO - 10.1016/j.jmb.2010.01.002
M3 - Journal article
C2 - 20064524
SN - 0022-2836
VL - 396
SP - 849
EP - 857
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
IS - 4
ER -