Quantitation of RHD by real-time polymerase chain reaction for determination of RHD zygosity and RHD mosaicism/chimerism: an evaluation of four quantitative methods

Grethe Risum Krog; Frederik Banch Clausen; Morten Hanefeld Dziegiel

doi:10.1111/j.1537-2995.2007.01175.x

Quantitation of RHD by real-time polymerase chain reaction for determination of RHD zygosity and RHD mosaicism/chimerism: an evaluation of four quantitative methods

Grethe Risum Krog, Frederik Banch Clausen, Morten Hanefeld Dziegiel

14 Citations (Scopus)

Abstract

Determination of RHD zygosity of the spouse is crucial in preconception counseling of families with history of hemolytic disease of the fetus and newborn caused by anti-D. RHD zygosity can be determined by quantitative real-time polymerase chain reaction (PCR) basically by determining RHD dosage, and this feature is relevant in investigating RHD mosaicism and chimerism.

Original language	English
Journal	Transfusion
Volume	47
Issue number	4
Pages (from-to)	715-22
Number of pages	8
ISSN	0041-1132
DOIs	https://doi.org/10.1111/j.1537-2995.2007.01175.x
Publication status	Published - Apr 2007

Keywords

Aged, 80 and over
Chimerism
Genotype
Heterozygote
Homozygote
Humans
Male
Mosaicism
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Reproducibility of Results
Rh-Hr Blood-Group System

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10.1111/j.1537-2995.2007.01175.x

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Quantitation of RHD by real-time polymerase chain reaction for determination of RHD zygosity and RHD mosaicism/chimerism : an evaluation of four quantitative methods. / Krog, Grethe Risum; Clausen, Frederik Banch; Dziegiel, Morten Hanefeld.

In: Transfusion, Vol. 47, No. 4, 04.2007, p. 715-22.

Research output: Contribution to journal › Journal article › Research › peer-review

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abstract = "Determination of RHD zygosity of the spouse is crucial in preconception counseling of families with history of hemolytic disease of the fetus and newborn caused by anti-D. RHD zygosity can be determined by quantitative real-time polymerase chain reaction (PCR) basically by determining RHD dosage, and this feature is relevant in investigating RHD mosaicism and chimerism.",

keywords = "Aged, 80 and over, Chimerism, Genotype, Heterozygote, Homozygote, Humans, Male, Mosaicism, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length, Reproducibility of Results, Rh-Hr Blood-Group System",

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AU - Dziegiel, Morten Hanefeld

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AB - Determination of RHD zygosity of the spouse is crucial in preconception counseling of families with history of hemolytic disease of the fetus and newborn caused by anti-D. RHD zygosity can be determined by quantitative real-time polymerase chain reaction (PCR) basically by determining RHD dosage, and this feature is relevant in investigating RHD mosaicism and chimerism.

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KW - Genotype

KW - Heterozygote

KW - Homozygote

KW - Humans

KW - Male

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KW - Polymorphism, Restriction Fragment Length

KW - Reproducibility of Results

KW - Rh-Hr Blood-Group System

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DO - 10.1111/j.1537-2995.2007.01175.x

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JO - Transfusion

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IS - 4

ER -

Quantitation of RHD by real-time polymerase chain reaction for determination of RHD zygosity and RHD mosaicism/chimerism: an evaluation of four quantitative methods

Abstract

Keywords

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