Proteomic analysis of the purple sulfur bacterium Candidatus "Thiodictyon syntrophicum" strain Cad16T isolated from Lake Cadagno

Nicola Storelli; Maged M. Saad; Niels-Ulrik Frigaard; Xavier Perret; Mauro Tonolla

doi:10.1016/j.euprot.2013.11.010

Proteomic analysis of the purple sulfur bacterium Candidatus "Thiodictyon syntrophicum" strain Cad16^T isolated from Lake Cadagno

Nicola Storelli^*, Maged M. Saad, Niels-Ulrik Frigaard, Xavier Perret, Mauro Tonolla

^*Corresponding author for this work

Marine Biology

7 Citations (Scopus)

773 Downloads (Pure)

Abstract

Lake Cadagno is characterised by a compact chemocline with high concentrations of purple sulfur bacteria (PSB). 2D-DIGE was used to monitor the global changes in the proteome of Candidatus "Thiodictyon syntrophicum" strain Cad16^T both in the presence and absence of light. This study aimed to disclose details regarding the dark CO₂ assimilation of the PSB, as this mechanism is often observed but is not yet sufficiently understood. Our results showed the presence of 17 protein spots that were more abundant in the dark, including three enzymes that could be part of the autotrophic dicarboxylate/4-hydroxybutyrate cycle, normally observed in archaea.

Original language	English
Journal	EuPA Open Proteomics
Volume	2
Pages (from-to)	17-30
Number of pages	14
ISSN	2212-9685
DOIs	https://doi.org/10.1016/j.euprot.2013.11.010
Publication status	Published - 2014

Keywords

Dicarboxylate/4-hydroxybutyrate (DC/HB) cycle
Granules of poly(3-hydroxybutyrate) (PHB)
Phototrophic sulfur bacteria

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10.1016/j.euprot.2013.11.010

Proteomic analysis of the purple sulfur bacterium Candidatus "Thiodictyon syntrophicum" strain Cad16T isolated from Lake CadagnoFinal published version, 877 KB

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title = "Proteomic analysis of the purple sulfur bacterium Candidatus {"}Thiodictyon syntrophicum{"} strain Cad16T isolated from Lake Cadagno",

abstract = "Lake Cadagno is characterised by a compact chemocline with high concentrations of purple sulfur bacteria (PSB). 2D-DIGE was used to monitor the global changes in the proteome of Candidatus {"}Thiodictyon syntrophicum{"} strain Cad16T both in the presence and absence of light. This study aimed to disclose details regarding the dark CO2 assimilation of the PSB, as this mechanism is often observed but is not yet sufficiently understood. Our results showed the presence of 17 protein spots that were more abundant in the dark, including three enzymes that could be part of the autotrophic dicarboxylate/4-hydroxybutyrate cycle, normally observed in archaea.",

keywords = "Dicarboxylate/4-hydroxybutyrate (DC/HB) cycle, Granules of poly(3-hydroxybutyrate) (PHB), Phototrophic sulfur bacteria",

author = "Nicola Storelli and Saad, {Maged M.} and Niels-Ulrik Frigaard and Xavier Perret and Mauro Tonolla",

year = "2014",

doi = "10.1016/j.euprot.2013.11.010",

language = "English",

volume = "2",

pages = "17--30",

journal = "EuPA Open Proteomics",

issn = "2212-9685",

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TY - JOUR

T1 - Proteomic analysis of the purple sulfur bacterium Candidatus "Thiodictyon syntrophicum" strain Cad16T isolated from Lake Cadagno

AU - Storelli, Nicola

AU - Saad, Maged M.

AU - Frigaard, Niels-Ulrik

AU - Perret, Xavier

AU - Tonolla, Mauro

PY - 2014

Y1 - 2014

N2 - Lake Cadagno is characterised by a compact chemocline with high concentrations of purple sulfur bacteria (PSB). 2D-DIGE was used to monitor the global changes in the proteome of Candidatus "Thiodictyon syntrophicum" strain Cad16T both in the presence and absence of light. This study aimed to disclose details regarding the dark CO2 assimilation of the PSB, as this mechanism is often observed but is not yet sufficiently understood. Our results showed the presence of 17 protein spots that were more abundant in the dark, including three enzymes that could be part of the autotrophic dicarboxylate/4-hydroxybutyrate cycle, normally observed in archaea.

AB - Lake Cadagno is characterised by a compact chemocline with high concentrations of purple sulfur bacteria (PSB). 2D-DIGE was used to monitor the global changes in the proteome of Candidatus "Thiodictyon syntrophicum" strain Cad16T both in the presence and absence of light. This study aimed to disclose details regarding the dark CO2 assimilation of the PSB, as this mechanism is often observed but is not yet sufficiently understood. Our results showed the presence of 17 protein spots that were more abundant in the dark, including three enzymes that could be part of the autotrophic dicarboxylate/4-hydroxybutyrate cycle, normally observed in archaea.

KW - Dicarboxylate/4-hydroxybutyrate (DC/HB) cycle

KW - Granules of poly(3-hydroxybutyrate) (PHB)

KW - Phototrophic sulfur bacteria

U2 - 10.1016/j.euprot.2013.11.010

DO - 10.1016/j.euprot.2013.11.010

M3 - Journal article

AN - SCOPUS:84895524782

SN - 2212-9685

VL - 2

SP - 17

EP - 30

JO - EuPA Open Proteomics

JF - EuPA Open Proteomics

ER -