TY - JOUR
T1 - Proteomic analysis of the purple sulfur bacterium Candidatus "Thiodictyon syntrophicum" strain Cad16T isolated from Lake Cadagno
AU - Storelli, Nicola
AU - Saad, Maged M.
AU - Frigaard, Niels-Ulrik
AU - Perret, Xavier
AU - Tonolla, Mauro
PY - 2014
Y1 - 2014
N2 - Lake Cadagno is characterised by a compact chemocline with high concentrations of purple sulfur bacteria (PSB). 2D-DIGE was used to monitor the global changes in the proteome of Candidatus "Thiodictyon syntrophicum" strain Cad16T both in the presence and absence of light. This study aimed to disclose details regarding the dark CO2 assimilation of the PSB, as this mechanism is often observed but is not yet sufficiently understood. Our results showed the presence of 17 protein spots that were more abundant in the dark, including three enzymes that could be part of the autotrophic dicarboxylate/4-hydroxybutyrate cycle, normally observed in archaea.
AB - Lake Cadagno is characterised by a compact chemocline with high concentrations of purple sulfur bacteria (PSB). 2D-DIGE was used to monitor the global changes in the proteome of Candidatus "Thiodictyon syntrophicum" strain Cad16T both in the presence and absence of light. This study aimed to disclose details regarding the dark CO2 assimilation of the PSB, as this mechanism is often observed but is not yet sufficiently understood. Our results showed the presence of 17 protein spots that were more abundant in the dark, including three enzymes that could be part of the autotrophic dicarboxylate/4-hydroxybutyrate cycle, normally observed in archaea.
KW - Dicarboxylate/4-hydroxybutyrate (DC/HB) cycle
KW - Granules of poly(3-hydroxybutyrate) (PHB)
KW - Phototrophic sulfur bacteria
U2 - 10.1016/j.euprot.2013.11.010
DO - 10.1016/j.euprot.2013.11.010
M3 - Journal article
AN - SCOPUS:84895524782
SN - 2212-9685
VL - 2
SP - 17
EP - 30
JO - EuPA Open Proteomics
JF - EuPA Open Proteomics
ER -