Proteins with GGDEF and EAL domains regulate Pseudomonas putida biofilm formation and dispersal.

TY - JOUR

T1 - Proteins with GGDEF and EAL domains regulate Pseudomonas putida biofilm formation and dispersal.

AU - Gjermansen, Morten

AU - Ragas, Paula

AU - Tolker-Nielsen, Tim

N1 - Keywords: Amino Acid Motifs; Bacterial Proteins; Biofilms; Cell Adhesion; Cell Movement; Escherichia coli; Gene Expression Regulation, Bacterial; Mutagenesis, Site-Directed; Protein Structure, Tertiary; Pseudomonas putida

PY - 2006

Y1 - 2006

N2 - Microbial biofilm formation often causes problems in medical and industrial settings, and knowledge about the factors that are involved in biofilm development and dispersion is useful for creating strategies to control the processes. In this report, we present evidence that proteins with GGDEF and EAL domains are involved in the regulation of biofilm formation and biofilm dispersion in Pseudomonas putida. Overexpression in P. putida of the Escherichia coli YedQ protein, which contains a GGDEF domain, resulted in increased biofilm formation. Overexpression in P. putida of the E. coli YhjH protein, which contains an EAL domain, strongly inhibited biofilm formation. Induction of YhjH expression in P. putida cells situated in established biofilms led to rapid dispersion of the biofilms. These results support the emerging theme that GGDEF-domain and EAL-domain proteins are involved in regulating the transition of bacteria between a roaming lifestyle and a sessile biofilm lifestyle.

AB - Microbial biofilm formation often causes problems in medical and industrial settings, and knowledge about the factors that are involved in biofilm development and dispersion is useful for creating strategies to control the processes. In this report, we present evidence that proteins with GGDEF and EAL domains are involved in the regulation of biofilm formation and biofilm dispersion in Pseudomonas putida. Overexpression in P. putida of the Escherichia coli YedQ protein, which contains a GGDEF domain, resulted in increased biofilm formation. Overexpression in P. putida of the E. coli YhjH protein, which contains an EAL domain, strongly inhibited biofilm formation. Induction of YhjH expression in P. putida cells situated in established biofilms led to rapid dispersion of the biofilms. These results support the emerging theme that GGDEF-domain and EAL-domain proteins are involved in regulating the transition of bacteria between a roaming lifestyle and a sessile biofilm lifestyle.

U2 - 10.1111/j.1574-6968.2006.00493.x

DO - 10.1111/j.1574-6968.2006.00493.x

M3 - Journal article

C2 - 17054717

SN - 0378-1097

VL - 265

SP - 215

EP - 224

JO - F E M S Microbiology Letters

JF - F E M S Microbiology Letters

IS - 2

ER -