PRE-mediated bypass of two Su(Hw) insulators targets PcG proteins to a downstream promoter

Itys Comet; Ekaterina Savitskaya; Bernd Schuettengruber; Nicolas Nègre; Sergey Lavrov; Aleksander Parshikov; François Juge; Elena Gracheva; Pavel Georgiev; Giacomo Cavalli

doi:10.1016/j.devcel.2006.05.009

PRE-mediated bypass of two Su(Hw) insulators targets PcG proteins to a downstream promoter

Itys Comet, Ekaterina Savitskaya, Bernd Schuettengruber, Nicolas Nègre, Sergey Lavrov, Aleksander Parshikov, François Juge, Elena Gracheva, Pavel Georgiev, Giacomo Cavalli

69 Citations (Scopus)

Abstract

Drosophila Polycomb group response elements (PRE) silence neighboring genes, but silencing can be blocked by one copy of the Su(Hw) insulator element. We show here that Polycomb group (PcG) proteins can spread from a PRE in the flanking chromatin region and that PRE blocking depends on a physical barrier established by the insulator to PcG protein spreading. On the other hand, PRE-mediated silencing can bypass two Su(Hw) insulators to repress a downstream reporter gene. Strikingly, insulator bypass involves targeting of PcG proteins to the downstream promoter, while they are completely excluded from the intervening insulated domain. This shows that PRE-dependent silencing is compatible with looping of the PRE in order to bring PcG proteins in contact with the promoter and does not require the coating of the whole chromatin domain between PRE and promoter.

Original language	English
Journal	Developmental Cell
Volume	11
Issue number	1
Pages (from-to)	117-24
Number of pages	7
ISSN	1534-5807
DOIs	https://doi.org/10.1016/j.devcel.2006.05.009
Publication status	Published - 2006
Externally published	Yes

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@article{402cdcb06d4011de8bc9000ea68e967b,

title = "PRE-mediated bypass of two Su(Hw) insulators targets PcG proteins to a downstream promoter",

abstract = "Drosophila Polycomb group response elements (PRE) silence neighboring genes, but silencing can be blocked by one copy of the Su(Hw) insulator element. We show here that Polycomb group (PcG) proteins can spread from a PRE in the flanking chromatin region and that PRE blocking depends on a physical barrier established by the insulator to PcG protein spreading. On the other hand, PRE-mediated silencing can bypass two Su(Hw) insulators to repress a downstream reporter gene. Strikingly, insulator bypass involves targeting of PcG proteins to the downstream promoter, while they are completely excluded from the intervening insulated domain. This shows that PRE-dependent silencing is compatible with looping of the PRE in order to bring PcG proteins in contact with the promoter and does not require the coating of the whole chromatin domain between PRE and promoter.",

author = "Itys Comet and Ekaterina Savitskaya and Bernd Schuettengruber and Nicolas N{\`e}gre and Sergey Lavrov and Aleksander Parshikov and Fran{\c c}ois Juge and Elena Gracheva and Pavel Georgiev and Giacomo Cavalli",

note = "Keywords: Animals; Animals, Genetically Modified; Binding Sites; DNA-Binding Proteins; Drosophila; Drosophila Proteins; Enhancer Elements, Genetic; Gene Silencing; Genes, Insect; Models, Biological; Promoter Regions, Genetic; Repressor Proteins",

year = "2006",

doi = "10.1016/j.devcel.2006.05.009",

language = "English",

volume = "11",

pages = "117--24",

journal = "Developmental Cell",

issn = "1534-5807",

publisher = "Cell Press",

number = "1",

}

TY - JOUR

T1 - PRE-mediated bypass of two Su(Hw) insulators targets PcG proteins to a downstream promoter

AU - Comet, Itys

AU - Savitskaya, Ekaterina

AU - Schuettengruber, Bernd

AU - Nègre, Nicolas

AU - Lavrov, Sergey

AU - Parshikov, Aleksander

AU - Juge, François

AU - Gracheva, Elena

AU - Georgiev, Pavel

AU - Cavalli, Giacomo

N1 - Keywords: Animals; Animals, Genetically Modified; Binding Sites; DNA-Binding Proteins; Drosophila; Drosophila Proteins; Enhancer Elements, Genetic; Gene Silencing; Genes, Insect; Models, Biological; Promoter Regions, Genetic; Repressor Proteins

PY - 2006

Y1 - 2006

N2 - Drosophila Polycomb group response elements (PRE) silence neighboring genes, but silencing can be blocked by one copy of the Su(Hw) insulator element. We show here that Polycomb group (PcG) proteins can spread from a PRE in the flanking chromatin region and that PRE blocking depends on a physical barrier established by the insulator to PcG protein spreading. On the other hand, PRE-mediated silencing can bypass two Su(Hw) insulators to repress a downstream reporter gene. Strikingly, insulator bypass involves targeting of PcG proteins to the downstream promoter, while they are completely excluded from the intervening insulated domain. This shows that PRE-dependent silencing is compatible with looping of the PRE in order to bring PcG proteins in contact with the promoter and does not require the coating of the whole chromatin domain between PRE and promoter.

AB - Drosophila Polycomb group response elements (PRE) silence neighboring genes, but silencing can be blocked by one copy of the Su(Hw) insulator element. We show here that Polycomb group (PcG) proteins can spread from a PRE in the flanking chromatin region and that PRE blocking depends on a physical barrier established by the insulator to PcG protein spreading. On the other hand, PRE-mediated silencing can bypass two Su(Hw) insulators to repress a downstream reporter gene. Strikingly, insulator bypass involves targeting of PcG proteins to the downstream promoter, while they are completely excluded from the intervening insulated domain. This shows that PRE-dependent silencing is compatible with looping of the PRE in order to bring PcG proteins in contact with the promoter and does not require the coating of the whole chromatin domain between PRE and promoter.

U2 - 10.1016/j.devcel.2006.05.009

DO - 10.1016/j.devcel.2006.05.009

M3 - Journal article

C2 - 16824958

SN - 1534-5807

VL - 11

SP - 117

EP - 124

JO - Developmental Cell

JF - Developmental Cell

IS - 1

ER -

PRE-mediated bypass of two Su(Hw) insulators targets PcG proteins to a downstream promoter

Abstract

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