TY - JOUR
T1 - PRE-mediated bypass of two Su(Hw) insulators targets PcG proteins to a downstream promoter
AU - Comet, Itys
AU - Savitskaya, Ekaterina
AU - Schuettengruber, Bernd
AU - Nègre, Nicolas
AU - Lavrov, Sergey
AU - Parshikov, Aleksander
AU - Juge, François
AU - Gracheva, Elena
AU - Georgiev, Pavel
AU - Cavalli, Giacomo
N1 - Keywords: Animals; Animals, Genetically Modified; Binding Sites; DNA-Binding Proteins; Drosophila; Drosophila Proteins; Enhancer Elements, Genetic; Gene Silencing; Genes, Insect; Models, Biological; Promoter Regions, Genetic; Repressor Proteins
PY - 2006
Y1 - 2006
N2 - Drosophila Polycomb group response elements (PRE) silence neighboring genes, but silencing can be blocked by one copy of the Su(Hw) insulator element. We show here that Polycomb group (PcG) proteins can spread from a PRE in the flanking chromatin region and that PRE blocking depends on a physical barrier established by the insulator to PcG protein spreading. On the other hand, PRE-mediated silencing can bypass two Su(Hw) insulators to repress a downstream reporter gene. Strikingly, insulator bypass involves targeting of PcG proteins to the downstream promoter, while they are completely excluded from the intervening insulated domain. This shows that PRE-dependent silencing is compatible with looping of the PRE in order to bring PcG proteins in contact with the promoter and does not require the coating of the whole chromatin domain between PRE and promoter.
AB - Drosophila Polycomb group response elements (PRE) silence neighboring genes, but silencing can be blocked by one copy of the Su(Hw) insulator element. We show here that Polycomb group (PcG) proteins can spread from a PRE in the flanking chromatin region and that PRE blocking depends on a physical barrier established by the insulator to PcG protein spreading. On the other hand, PRE-mediated silencing can bypass two Su(Hw) insulators to repress a downstream reporter gene. Strikingly, insulator bypass involves targeting of PcG proteins to the downstream promoter, while they are completely excluded from the intervening insulated domain. This shows that PRE-dependent silencing is compatible with looping of the PRE in order to bring PcG proteins in contact with the promoter and does not require the coating of the whole chromatin domain between PRE and promoter.
U2 - 10.1016/j.devcel.2006.05.009
DO - 10.1016/j.devcel.2006.05.009
M3 - Journal article
C2 - 16824958
SN - 1534-5807
VL - 11
SP - 117
EP - 124
JO - Developmental Cell
JF - Developmental Cell
IS - 1
ER -