Porcine ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a): cloning, transcription, expression, mapping, and identification of an NPP1/CD203a epitope for swine workshop cluster 9 (SWC9) monoclonal antibodies

Cathrine Bie Petersen; Ann-Britt Nygaard Hillig; Birgitte Viuff; Merete Fredholm; Bent Aasted; Jan Salomonsen

doi:10.1016/j.dci.2006.08.012

Porcine ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a): cloning, transcription, expression, mapping, and identification of an NPP1/CD203a epitope for swine workshop cluster 9 (SWC9) monoclonal antibodies

Cathrine Bie Petersen, Ann-Britt Nygaard Hillig, Birgitte Viuff, Merete Fredholm, Bent Aasted, Jan Salomonsen

12 Citations (Scopus)

Abstract

Swine workshop cluster 9 (SWC9) antibody identifying a porcien epitope on macrophages and thymocytes was used to precipitate and characterize the molecule from biotinylated macrophages and to obtain peptide sequence by mass spectrometry. The protein was identified as ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a). The porcine NPP1/CD203a encoding gene was mapped to chromosome 1 using a radiation hybrid panel, and transcription was investigated by RT-PCR analysis of several tissues. The cDNA was cloned and introduced into COS7 cells resulting in expression of functionally active enzyme and verification of the specificity of an SWC9 reacting monoclonal antibody. The antibody was used for immunohistochemical examination of various porcine tissues. Most prominent expression of NPP1/CD203a was found in lung macrophages and liver sinusoids.

Original language	English
Journal	Developmental & Comparative Immunology
Volume	31
Issue number	6
Pages (from-to)	618-631
Number of pages	14
ISSN	0145-305X
DOIs	https://doi.org/10.1016/j.dci.2006.08.012
Publication status	Published - 2007

Keywords

Former LIFE faculty
SWC9
NPP1
Swine
Macrophage

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Petersen, C. B., Hillig, A-B. N., Viuff, B., Fredholm, M., Aasted, B., & Salomonsen, J. (2007). Porcine ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a): cloning, transcription, expression, mapping, and identification of an NPP1/CD203a epitope for swine workshop cluster 9 (SWC9) monoclonal antibodies. Developmental & Comparative Immunology, 31(6), 618-631. https://doi.org/10.1016/j.dci.2006.08.012

Porcine ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a) : cloning, transcription, expression, mapping, and identification of an NPP1/CD203a epitope for swine workshop cluster 9 (SWC9) monoclonal antibodies. / Petersen, Cathrine Bie; Hillig, Ann-Britt Nygaard; Viuff, Birgitte et al.

In: Developmental & Comparative Immunology, Vol. 31, No. 6, 2007, p. 618-631.

Research output: Contribution to journal › Journal article › Research › peer-review

Petersen, CB, Hillig, A-BN, Viuff, B, Fredholm, M, Aasted, B & Salomonsen, J 2007, 'Porcine ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a): cloning, transcription, expression, mapping, and identification of an NPP1/CD203a epitope for swine workshop cluster 9 (SWC9) monoclonal antibodies', Developmental & Comparative Immunology, vol. 31, no. 6, pp. 618-631. https://doi.org/10.1016/j.dci.2006.08.012

Petersen CB, Hillig A-BN, Viuff B, Fredholm M, Aasted B, Salomonsen J. Porcine ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a): cloning, transcription, expression, mapping, and identification of an NPP1/CD203a epitope for swine workshop cluster 9 (SWC9) monoclonal antibodies. Developmental & Comparative Immunology. 2007;31(6):618-631. doi: 10.1016/j.dci.2006.08.012

Petersen, Cathrine Bie ; Hillig, Ann-Britt Nygaard ; Viuff, Birgitte et al. / Porcine ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a) : cloning, transcription, expression, mapping, and identification of an NPP1/CD203a epitope for swine workshop cluster 9 (SWC9) monoclonal antibodies. In: Developmental & Comparative Immunology. 2007 ; Vol. 31, No. 6. pp. 618-631.

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abstract = "Swine workshop cluster 9 (SWC9) antibody identifying a porcien epitope on macrophages and thymocytes was used to precipitate and characterize the molecule from biotinylated macrophages and to obtain peptide sequence by mass spectrometry. The protein was identified as ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a). The porcine NPP1/CD203a encoding gene was mapped to chromosome 1 using a radiation hybrid panel, and transcription was investigated by RT-PCR analysis of several tissues. The cDNA was cloned and introduced into COS7 cells resulting in expression of functionally active enzyme and verification of the specificity of an SWC9 reacting monoclonal antibody. The antibody was used for immunohistochemical examination of various porcine tissues. Most prominent expression of NPP1/CD203a was found in lung macrophages and liver sinusoids.",

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