Porcine ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a): cloning, transcription, expression, mapping, and identification of an NPP1/CD203a epitope for swine workshop cluster 9 (SWC9) monoclonal antibodies

Cathrine Bie Petersen; Ann-Britt Nygaard Hillig; Birgitte Viuff; Merete Fredholm; Bent Aasted; Jan Salomonsen

doi:10.1016/j.dci.2006.08.012

Porcine ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a): cloning, transcription, expression, mapping, and identification of an NPP1/CD203a epitope for swine workshop cluster 9 (SWC9) monoclonal antibodies

Cathrine Bie Petersen, Ann-Britt Nygaard Hillig, Birgitte Viuff, Merete Fredholm, Bent Aasted, Jan Salomonsen

12 Citationer (Scopus)

Abstract

Swine workshop cluster 9 (SWC9) antibody identifying a porcien epitope on macrophages and thymocytes was used to precipitate and characterize the molecule from biotinylated macrophages and to obtain peptide sequence by mass spectrometry. The protein was identified as ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a). The porcine NPP1/CD203a encoding gene was mapped to chromosome 1 using a radiation hybrid panel, and transcription was investigated by RT-PCR analysis of several tissues. The cDNA was cloned and introduced into COS7 cells resulting in expression of functionally active enzyme and verification of the specificity of an SWC9 reacting monoclonal antibody. The antibody was used for immunohistochemical examination of various porcine tissues. Most prominent expression of NPP1/CD203a was found in lung macrophages and liver sinusoids.

Originalsprog	Engelsk
Tidsskrift	Developmental & Comparative Immunology
Vol/bind	31
Udgave nummer	6
Sider (fra-til)	618-631
Antal sider	14
ISSN	0145-305X
DOI	https://doi.org/10.1016/j.dci.2006.08.012
Status	Udgivet - 2007

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Citationsformater

Petersen, C. B., Hillig, A-B. N., Viuff, B., Fredholm, M., Aasted, B., & Salomonsen, J. (2007). Porcine ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a): cloning, transcription, expression, mapping, and identification of an NPP1/CD203a epitope for swine workshop cluster 9 (SWC9) monoclonal antibodies. Developmental & Comparative Immunology, 31(6), 618-631. https://doi.org/10.1016/j.dci.2006.08.012

Porcine ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a) : cloning, transcription, expression, mapping, and identification of an NPP1/CD203a epitope for swine workshop cluster 9 (SWC9) monoclonal antibodies. / Petersen, Cathrine Bie; Hillig, Ann-Britt Nygaard; Viuff, Birgitte et al.

I: Developmental & Comparative Immunology, Bind 31, Nr. 6, 2007, s. 618-631.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Petersen, CB, Hillig, A-BN, Viuff, B, Fredholm, M, Aasted, B & Salomonsen, J 2007, 'Porcine ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a): cloning, transcription, expression, mapping, and identification of an NPP1/CD203a epitope for swine workshop cluster 9 (SWC9) monoclonal antibodies', Developmental & Comparative Immunology, bind 31, nr. 6, s. 618-631. https://doi.org/10.1016/j.dci.2006.08.012

Petersen CB, Hillig A-BN, Viuff B, Fredholm M, Aasted B, Salomonsen J. Porcine ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a): cloning, transcription, expression, mapping, and identification of an NPP1/CD203a epitope for swine workshop cluster 9 (SWC9) monoclonal antibodies. Developmental & Comparative Immunology. 2007;31(6):618-631. doi: 10.1016/j.dci.2006.08.012

Petersen, Cathrine Bie ; Hillig, Ann-Britt Nygaard ; Viuff, Birgitte et al. / Porcine ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a) : cloning, transcription, expression, mapping, and identification of an NPP1/CD203a epitope for swine workshop cluster 9 (SWC9) monoclonal antibodies. I: Developmental & Comparative Immunology. 2007 ; Bind 31, Nr. 6. s. 618-631.

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abstract = "Swine workshop cluster 9 (SWC9) antibody identifying a porcien epitope on macrophages and thymocytes was used to precipitate and characterize the molecule from biotinylated macrophages and to obtain peptide sequence by mass spectrometry. The protein was identified as ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a). The porcine NPP1/CD203a encoding gene was mapped to chromosome 1 using a radiation hybrid panel, and transcription was investigated by RT-PCR analysis of several tissues. The cDNA was cloned and introduced into COS7 cells resulting in expression of functionally active enzyme and verification of the specificity of an SWC9 reacting monoclonal antibody. The antibody was used for immunohistochemical examination of various porcine tissues. Most prominent expression of NPP1/CD203a was found in lung macrophages and liver sinusoids.",

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AU - Petersen, Cathrine Bie

AU - Hillig, Ann-Britt Nygaard

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AU - Aasted, Bent

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AB - Swine workshop cluster 9 (SWC9) antibody identifying a porcien epitope on macrophages and thymocytes was used to precipitate and characterize the molecule from biotinylated macrophages and to obtain peptide sequence by mass spectrometry. The protein was identified as ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1/CD203a). The porcine NPP1/CD203a encoding gene was mapped to chromosome 1 using a radiation hybrid panel, and transcription was investigated by RT-PCR analysis of several tissues. The cDNA was cloned and introduced into COS7 cells resulting in expression of functionally active enzyme and verification of the specificity of an SWC9 reacting monoclonal antibody. The antibody was used for immunohistochemical examination of various porcine tissues. Most prominent expression of NPP1/CD203a was found in lung macrophages and liver sinusoids.

KW - Former LIFE faculty

KW - SWC9

KW - NPP1

KW - Swine

KW - Macrophage

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DO - 10.1016/j.dci.2006.08.012

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