Placental transport and in vitro effects of Bisphenol A

Thit J Mørck; Giuseppina Sorda; Nicoletta Bechi; Brian Schou Rasmussen; Jesper Bo Nielsen; Francesca Ietta; Erik Rytting; Line Mathiesen; Luana Paulesu; Lisbeth E Knudsen

doi:10.1016/j.reprotox.2010.02.007

Placental transport and in vitro effects of Bisphenol A

Thit J Mørck, Giuseppina Sorda, Nicoletta Bechi, Brian Schou Rasmussen, Jesper Bo Nielsen, Francesca Ietta, Erik Rytting, Line Mathiesen, Luana Paulesu, Lisbeth E Knudsen

133 Citations (Scopus)

Abstract

Bisphenol A (BPA), an estrogen-like chemical, leaches from consumer products potentially causing human exposure. To examine the effects of BPA exposure during pregnancy, we performed studies using the BeWo trophoblast cell line, placental explant cultures, placental perfusions and skin diffusion models, all of human origin. Results showed BPA cytotoxicity in BeWo cells with an apparent EC50 at 100-125. μM. BPA exposure significantly increased β-hCG secretion and caspase-3 expression in placental explants at an environmentally relevant concentration of 1. nM. In the transport studies, a rapid transfer of BPA was observed across the term placentae and the BeWo cell monolayer. Further, transdermal transport of BPA was observed. These results indicate that fetal BPA exposure through placental exchange occurs with potential adverse implications for placental and fetal development. This battery of test systems within the realm of human implantation and fetal development represents important elements in risk assessment of reproductive toxicity.

Original language	English
Journal	Reproductive Toxicology
Volume	30
Pages (from-to)	131-137
ISSN	0890-6238
DOIs	https://doi.org/10.1016/j.reprotox.2010.02.007
Publication status	Published - Aug 2010

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10.1016/j.reprotox.2010.02.007

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@article{eb88c0e0834e11df928f000ea68e967b,

title = "Placental transport and in vitro effects of Bisphenol A",

abstract = "Bisphenol A (BPA), an estrogen-like chemical, leaches from consumer products potentially causing human exposure. To examine the effects of BPA exposure during pregnancy, we performed studies using the BeWo trophoblast cell line, placental explant cultures, placental perfusions and skin diffusion models, all of human origin. Results showed BPA cytotoxicity in BeWo cells with an apparent EC50 at 100-125. μM. BPA exposure significantly increased β-hCG secretion and caspase-3 expression in placental explants at an environmentally relevant concentration of 1. nM. In the transport studies, a rapid transfer of BPA was observed across the term placentae and the BeWo cell monolayer. Further, transdermal transport of BPA was observed. These results indicate that fetal BPA exposure through placental exchange occurs with potential adverse implications for placental and fetal development. This battery of test systems within the realm of human implantation and fetal development represents important elements in risk assessment of reproductive toxicity.",

author = "M{\o}rck, {Thit J} and Giuseppina Sorda and Nicoletta Bechi and Rasmussen, {Brian Schou} and Nielsen, {Jesper Bo} and Francesca Ietta and Erik Rytting and Line Mathiesen and Luana Paulesu and Knudsen, {Lisbeth E}",

year = "2010",

month = aug,

doi = "10.1016/j.reprotox.2010.02.007",

language = "English",

volume = "30",

pages = "131--137",

journal = "Reproductive Toxicology",

issn = "0890-6238",

publisher = "Elsevier",

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TY - JOUR

T1 - Placental transport and in vitro effects of Bisphenol A

AU - Mørck, Thit J

AU - Sorda, Giuseppina

AU - Bechi, Nicoletta

AU - Rasmussen, Brian Schou

AU - Nielsen, Jesper Bo

AU - Ietta, Francesca

AU - Rytting, Erik

AU - Mathiesen, Line

AU - Paulesu, Luana

AU - Knudsen, Lisbeth E

PY - 2010/8

Y1 - 2010/8

N2 - Bisphenol A (BPA), an estrogen-like chemical, leaches from consumer products potentially causing human exposure. To examine the effects of BPA exposure during pregnancy, we performed studies using the BeWo trophoblast cell line, placental explant cultures, placental perfusions and skin diffusion models, all of human origin. Results showed BPA cytotoxicity in BeWo cells with an apparent EC50 at 100-125. μM. BPA exposure significantly increased β-hCG secretion and caspase-3 expression in placental explants at an environmentally relevant concentration of 1. nM. In the transport studies, a rapid transfer of BPA was observed across the term placentae and the BeWo cell monolayer. Further, transdermal transport of BPA was observed. These results indicate that fetal BPA exposure through placental exchange occurs with potential adverse implications for placental and fetal development. This battery of test systems within the realm of human implantation and fetal development represents important elements in risk assessment of reproductive toxicity.

AB - Bisphenol A (BPA), an estrogen-like chemical, leaches from consumer products potentially causing human exposure. To examine the effects of BPA exposure during pregnancy, we performed studies using the BeWo trophoblast cell line, placental explant cultures, placental perfusions and skin diffusion models, all of human origin. Results showed BPA cytotoxicity in BeWo cells with an apparent EC50 at 100-125. μM. BPA exposure significantly increased β-hCG secretion and caspase-3 expression in placental explants at an environmentally relevant concentration of 1. nM. In the transport studies, a rapid transfer of BPA was observed across the term placentae and the BeWo cell monolayer. Further, transdermal transport of BPA was observed. These results indicate that fetal BPA exposure through placental exchange occurs with potential adverse implications for placental and fetal development. This battery of test systems within the realm of human implantation and fetal development represents important elements in risk assessment of reproductive toxicity.

U2 - 10.1016/j.reprotox.2010.02.007

DO - 10.1016/j.reprotox.2010.02.007

M3 - Journal article

C2 - 20214975

SN - 0890-6238

VL - 30

SP - 131

EP - 137

JO - Reproductive Toxicology

JF - Reproductive Toxicology

ER -

Placental transport and in vitro effects of Bisphenol A

Abstract

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