TY - JOUR
T1 - Placental transport and in vitro effects of Bisphenol A
AU - Mørck, Thit J
AU - Sorda, Giuseppina
AU - Bechi, Nicoletta
AU - Rasmussen, Brian Schou
AU - Nielsen, Jesper Bo
AU - Ietta, Francesca
AU - Rytting, Erik
AU - Mathiesen, Line
AU - Paulesu, Luana
AU - Knudsen, Lisbeth E
N1 - Copyright © 2010 Elsevier Inc. All rights reserved.
PY - 2010/8
Y1 - 2010/8
N2 - Bisphenol A (BPA), an estrogen-like chemical, leaches from consumer products potentially causing human exposure. To examine the effects of BPA exposure during pregnancy, we performed studies using the BeWo trophoblast cell line, placental explant cultures, placental perfusions and skin diffusion models, all of human origin. Results showed BPA cytotoxicity in BeWo cells with an apparent EC50 at 100-125. μM. BPA exposure significantly increased β-hCG secretion and caspase-3 expression in placental explants at an environmentally relevant concentration of 1. nM. In the transport studies, a rapid transfer of BPA was observed across the term placentae and the BeWo cell monolayer. Further, transdermal transport of BPA was observed. These results indicate that fetal BPA exposure through placental exchange occurs with potential adverse implications for placental and fetal development. This battery of test systems within the realm of human implantation and fetal development represents important elements in risk assessment of reproductive toxicity.
AB - Bisphenol A (BPA), an estrogen-like chemical, leaches from consumer products potentially causing human exposure. To examine the effects of BPA exposure during pregnancy, we performed studies using the BeWo trophoblast cell line, placental explant cultures, placental perfusions and skin diffusion models, all of human origin. Results showed BPA cytotoxicity in BeWo cells with an apparent EC50 at 100-125. μM. BPA exposure significantly increased β-hCG secretion and caspase-3 expression in placental explants at an environmentally relevant concentration of 1. nM. In the transport studies, a rapid transfer of BPA was observed across the term placentae and the BeWo cell monolayer. Further, transdermal transport of BPA was observed. These results indicate that fetal BPA exposure through placental exchange occurs with potential adverse implications for placental and fetal development. This battery of test systems within the realm of human implantation and fetal development represents important elements in risk assessment of reproductive toxicity.
U2 - 10.1016/j.reprotox.2010.02.007
DO - 10.1016/j.reprotox.2010.02.007
M3 - Journal article
C2 - 20214975
SN - 0890-6238
VL - 30
SP - 131
EP - 137
JO - Reproductive Toxicology
JF - Reproductive Toxicology
ER -