Performance of two 17 locus forensic identification STR kits - Applied Biosystems's AmpFlSTR® NGMSElect(tm) and Promega's PowerPlex® ESI17 kits

TY - JOUR

T1 - Performance of two 17 locus forensic identification STR kits - Applied Biosystems's AmpFlSTR® NGMSElect(tm) and Promega's PowerPlex® ESI17 kits

AU - Tvedebrink, Torben

AU - Mogensen, Helle Smidt

AU - Stene, Maria Charlotte Aslaug

AU - Morling, Niels

PY - 2012/9

Y1 - 2012/9

N2 - We compared the performance of two recently released 17 loci STR multiplexes for human identification: Applied Biosystems's AmpFℓSTR® NGMSElect™ and Promega's PowerPlex® ESI17. The comparative parameters were chosen by their relevance in forensic identification and particularly in crime cases. The comparative analyses encompass: amplification ability, heterozygote balance, allelic drop-out, drop-in, stutter analysis and inter-locus balance. Four DNA profiles were analysed in various concentrations in a serial dilution experiment. The amounts of DNA in the PCR ranged from 3 pg to 420 pg and were analysed in triplicate using 28, 29 and 30 PCR cycles. In order to compare the kits, aliquots from each sample were analysed with both kits under identical conditions. Furthermore, DNA profiles from 200 reference profiles were analysed using both kits. The results from the statistical analyses did not indicate any substantial differences of practical relevance between the kits for forensic case work. For all parameters included in this comparative study, the two kits showed no departure from previously observed patterns relative to e.g. the amounts of DNA or amplicon lengths. Based on our analyses, both kits are considered applicable for forensic crime case work.

AB - We compared the performance of two recently released 17 loci STR multiplexes for human identification: Applied Biosystems's AmpFℓSTR® NGMSElect™ and Promega's PowerPlex® ESI17. The comparative parameters were chosen by their relevance in forensic identification and particularly in crime cases. The comparative analyses encompass: amplification ability, heterozygote balance, allelic drop-out, drop-in, stutter analysis and inter-locus balance. Four DNA profiles were analysed in various concentrations in a serial dilution experiment. The amounts of DNA in the PCR ranged from 3 pg to 420 pg and were analysed in triplicate using 28, 29 and 30 PCR cycles. In order to compare the kits, aliquots from each sample were analysed with both kits under identical conditions. Furthermore, DNA profiles from 200 reference profiles were analysed using both kits. The results from the statistical analyses did not indicate any substantial differences of practical relevance between the kits for forensic case work. For all parameters included in this comparative study, the two kits showed no departure from previously observed patterns relative to e.g. the amounts of DNA or amplicon lengths. Based on our analyses, both kits are considered applicable for forensic crime case work.

U2 - 10.1016/j.fsigen.2011.12.006

DO - 10.1016/j.fsigen.2011.12.006

M3 - Journal article

SN - 1872-4973

VL - 6

SP - 523

EP - 531

JO - Forensic Science International: Genetics

JF - Forensic Science International: Genetics

IS - 5

ER -