Performance of PCR-restriction fragment length polymorphism analysis of the Helicobacter pylori ureB gene in differentiating gene variants.

H Colding; S H Hartzen; M Mohammadi; K Herup; L P Andersen; Jette E. Kristiansen

Performance of PCR-restriction fragment length polymorphism analysis of the Helicobacter pylori ureB gene in differentiating gene variants.

H Colding, S H Hartzen, M Mohammadi, K Herup, L P Andersen, Jette E. Kristiansen

2 Citations (Scopus)

Abstract

Recently, PCR-restriction fragment length polymorphism (PCR-RFLP) of the urease genes of Helicobacter pylori was evaluated in a meta-analysis; acceptable discriminatory indices of the ureAB and C genes were found. In the present investigation, we found a discriminatory index of 0.95 for 191 unrelated clinical H. pylori isolates with PCR-RFLP typing of the ureB gene (933 bp), combining the results obtained with restriction enzymes HaeIII and Sau3A, and a mixture of the enzymes. We therefore find that PCR-RFLP typing of the ureB gene of H. pylori with restriction enzymes HaeIII and Sau3A is comparable to typing of other H. pylori urease genes.

Original language	English
Journal	Clinical Microbiology and Infection
Volume	9
Issue number	1
Pages (from-to)	57-60
Number of pages	3
ISSN	1198-743X
Publication status	Published - 2003

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@article{a9ede680b54e11ddb04f000ea68e967b,

title = "Performance of PCR-restriction fragment length polymorphism analysis of the Helicobacter pylori ureB gene in differentiating gene variants.",

abstract = "Recently, PCR-restriction fragment length polymorphism (PCR-RFLP) of the urease genes of Helicobacter pylori was evaluated in a meta-analysis; acceptable discriminatory indices of the ureAB and C genes were found. In the present investigation, we found a discriminatory index of 0.95 for 191 unrelated clinical H. pylori isolates with PCR-RFLP typing of the ureB gene (933 bp), combining the results obtained with restriction enzymes HaeIII and Sau3A, and a mixture of the enzymes. We therefore find that PCR-RFLP typing of the ureB gene of H. pylori with restriction enzymes HaeIII and Sau3A is comparable to typing of other H. pylori urease genes.",

author = "H Colding and Hartzen, {S H} and M Mohammadi and K Herup and Andersen, {L P} and Kristiansen, {Jette E.}",

note = "Keywords: DNA, Bacterial; Deoxyribonucleases, Type II Site-Specific; Helicobacter pylori; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Urease",

year = "2003",

language = "English",

volume = "9",

pages = "57--60",

journal = "Clinical Microbiology and Infection",

issn = "1198-743X",

publisher = "Elsevier",

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TY - JOUR

T1 - Performance of PCR-restriction fragment length polymorphism analysis of the Helicobacter pylori ureB gene in differentiating gene variants.

AU - Colding, H

AU - Hartzen, S H

AU - Mohammadi, M

AU - Herup, K

AU - Andersen, L P

AU - Kristiansen, Jette E.

N1 - Keywords: DNA, Bacterial; Deoxyribonucleases, Type II Site-Specific; Helicobacter pylori; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Urease

PY - 2003

Y1 - 2003

N2 - Recently, PCR-restriction fragment length polymorphism (PCR-RFLP) of the urease genes of Helicobacter pylori was evaluated in a meta-analysis; acceptable discriminatory indices of the ureAB and C genes were found. In the present investigation, we found a discriminatory index of 0.95 for 191 unrelated clinical H. pylori isolates with PCR-RFLP typing of the ureB gene (933 bp), combining the results obtained with restriction enzymes HaeIII and Sau3A, and a mixture of the enzymes. We therefore find that PCR-RFLP typing of the ureB gene of H. pylori with restriction enzymes HaeIII and Sau3A is comparable to typing of other H. pylori urease genes.

AB - Recently, PCR-restriction fragment length polymorphism (PCR-RFLP) of the urease genes of Helicobacter pylori was evaluated in a meta-analysis; acceptable discriminatory indices of the ureAB and C genes were found. In the present investigation, we found a discriminatory index of 0.95 for 191 unrelated clinical H. pylori isolates with PCR-RFLP typing of the ureB gene (933 bp), combining the results obtained with restriction enzymes HaeIII and Sau3A, and a mixture of the enzymes. We therefore find that PCR-RFLP typing of the ureB gene of H. pylori with restriction enzymes HaeIII and Sau3A is comparable to typing of other H. pylori urease genes.

M3 - Journal article

C2 - 12691544

SN - 1198-743X

VL - 9

SP - 57

EP - 60

JO - Clinical Microbiology and Infection

JF - Clinical Microbiology and Infection

IS - 1

ER -

Performance of PCR-restriction fragment length polymorphism analysis of the Helicobacter pylori ureB gene in differentiating gene variants.

Abstract

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