@article{a9ede680b54e11ddb04f000ea68e967b,
title = "Performance of PCR-restriction fragment length polymorphism analysis of the Helicobacter pylori ureB gene in differentiating gene variants.",
abstract = "Recently, PCR-restriction fragment length polymorphism (PCR-RFLP) of the urease genes of Helicobacter pylori was evaluated in a meta-analysis; acceptable discriminatory indices of the ureAB and C genes were found. In the present investigation, we found a discriminatory index of 0.95 for 191 unrelated clinical H. pylori isolates with PCR-RFLP typing of the ureB gene (933 bp), combining the results obtained with restriction enzymes HaeIII and Sau3A, and a mixture of the enzymes. We therefore find that PCR-RFLP typing of the ureB gene of H. pylori with restriction enzymes HaeIII and Sau3A is comparable to typing of other H. pylori urease genes.",
author = "H Colding and Hartzen, {S H} and M Mohammadi and K Herup and Andersen, {L P} and Kristiansen, {Jette E.}",
note = "Keywords: DNA, Bacterial; Deoxyribonucleases, Type II Site-Specific; Helicobacter pylori; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Urease",
year = "2003",
language = "English",
volume = "9",
pages = "57--60",
journal = "Clinical Microbiology and Infection",
issn = "1198-743X",
publisher = "Elsevier",
number = "1",
}