PCR typing of two short tandem repeat (STR) structures upstreams of the human myelin basic protein (MBP) gene: The genetic susceptibility in multiple sclerosis and monosymptomatic idiopathic optic neuritis in Danes

L J Nellemann; J Frederiksen; N Morling

PCR typing of two short tandem repeat (STR) structures upstreams of the human myelin basic protein (MBP) gene: The genetic susceptibility in multiple sclerosis and monosymptomatic idiopathic optic neuritis in Danes

L J Nellemann, J Frederiksen, N Morling

8 Citations (Scopus)

Abstract

We investigated two short tandem tetranucleotide (TGGA) repeat polymorphisms upstreams of the myelin basic protein (MBP) gene. The region was amplified by the polymerase chain reaction (PCR) and the two repeat systems were separated by cutting with the restriction enzyme NlaIII. The lengths of the DNA fragments were analyzed by vertical electrophoresis in polyacrylamide gels followed by silver staining. We compared the DNA fragment frequencies of the two MBP regions in 34 patients suffering from multiple sclerosis and in 78 suffering from monosymptomatic idiopathic optic neuritis to those in 200 healthy controls. We found no significant differences between the MBP fragment frequencies in either of the patient groups and in the control group.

Original language	English
Journal	Multiple Sclerosis
Volume	1
Issue number	3
Pages (from-to)	186-9
Number of pages	3
ISSN	1352-4585
Publication status	Published - 1995

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PCR typing of two short tandem repeat (STR) structures upstreams of the human myelin basic protein (MBP) gene : The genetic susceptibility in multiple sclerosis and monosymptomatic idiopathic optic neuritis in Danes. / Nellemann, L J; Frederiksen, J; Morling, N.

In: Multiple Sclerosis, Vol. 1, No. 3, 1995, p. 186-9.

Research output: Contribution to journal › Journal article › Research › peer-review

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title = "PCR typing of two short tandem repeat (STR) structures upstreams of the human myelin basic protein (MBP) gene: The genetic susceptibility in multiple sclerosis and monosymptomatic idiopathic optic neuritis in Danes",

abstract = "We investigated two short tandem tetranucleotide (TGGA) repeat polymorphisms upstreams of the myelin basic protein (MBP) gene. The region was amplified by the polymerase chain reaction (PCR) and the two repeat systems were separated by cutting with the restriction enzyme NlaIII. The lengths of the DNA fragments were analyzed by vertical electrophoresis in polyacrylamide gels followed by silver staining. We compared the DNA fragment frequencies of the two MBP regions in 34 patients suffering from multiple sclerosis and in 78 suffering from monosymptomatic idiopathic optic neuritis to those in 200 healthy controls. We found no significant differences between the MBP fragment frequencies in either of the patient groups and in the control group.",

author = "Nellemann, {L J} and J Frederiksen and N Morling",

note = "Keywords: Alleles; Chromosome Deletion; Cloning, Molecular; DNA; Denmark; Gene Frequency; Genes; Genetic Predisposition to Disease; Humans; Microsatellite Repeats; Multiple Sclerosis; Myelin Basic Proteins; Optic Neuritis; Polymerase Chain Reaction",

year = "1995",

language = "English",

volume = "1",

pages = "186--9",

journal = "Multiple Sclerosis Journal",

issn = "1352-4585",

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number = "3",

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TY - JOUR

T1 - PCR typing of two short tandem repeat (STR) structures upstreams of the human myelin basic protein (MBP) gene

T2 - The genetic susceptibility in multiple sclerosis and monosymptomatic idiopathic optic neuritis in Danes

AU - Nellemann, L J

AU - Frederiksen, J

AU - Morling, N

N1 - Keywords: Alleles; Chromosome Deletion; Cloning, Molecular; DNA; Denmark; Gene Frequency; Genes; Genetic Predisposition to Disease; Humans; Microsatellite Repeats; Multiple Sclerosis; Myelin Basic Proteins; Optic Neuritis; Polymerase Chain Reaction

PY - 1995

Y1 - 1995

N2 - We investigated two short tandem tetranucleotide (TGGA) repeat polymorphisms upstreams of the myelin basic protein (MBP) gene. The region was amplified by the polymerase chain reaction (PCR) and the two repeat systems were separated by cutting with the restriction enzyme NlaIII. The lengths of the DNA fragments were analyzed by vertical electrophoresis in polyacrylamide gels followed by silver staining. We compared the DNA fragment frequencies of the two MBP regions in 34 patients suffering from multiple sclerosis and in 78 suffering from monosymptomatic idiopathic optic neuritis to those in 200 healthy controls. We found no significant differences between the MBP fragment frequencies in either of the patient groups and in the control group.

AB - We investigated two short tandem tetranucleotide (TGGA) repeat polymorphisms upstreams of the myelin basic protein (MBP) gene. The region was amplified by the polymerase chain reaction (PCR) and the two repeat systems were separated by cutting with the restriction enzyme NlaIII. The lengths of the DNA fragments were analyzed by vertical electrophoresis in polyacrylamide gels followed by silver staining. We compared the DNA fragment frequencies of the two MBP regions in 34 patients suffering from multiple sclerosis and in 78 suffering from monosymptomatic idiopathic optic neuritis to those in 200 healthy controls. We found no significant differences between the MBP fragment frequencies in either of the patient groups and in the control group.

M3 - Journal article

C2 - 9345452

SN - 1352-4585

VL - 1

SP - 186

EP - 189

JO - Multiple Sclerosis Journal

JF - Multiple Sclerosis Journal

IS - 3

ER -