Abstract
An optimized semi-automatic protocol for parallel synthesis of up to 96 peptide nucleic acids (PNA) or PNA-peptide conjugates using Boc-protection strategy has been developed using a robotic system. The approach is illustrated by synthesizing PNA and PNA-peptide libraries varying between 15 and 27 amino acid units. The peptides (NLS (nuclear localization signal) or Tat-peptide) were attached to N-terminus of the PNA. The method was found to be far superior to that based on the SPOT/Fmoc protocol by which PNA oligomers are synthesized on a modified cellulose membrane. On a 0.5 micromole scale the method typically yielded 2 mg product of 90% purity by HPLC/MALDI-TOF analysis. This approach is suitable for screening of a large number of PNA and/or peptide sequences for biochemical and biological studies.
| Original language | English |
|---|---|
| Journal | Combinatorial Chemistry & High Throughput Screening |
| Volume | 5 |
| Issue number | 3 |
| Pages (from-to) | 253-9 |
| Number of pages | 7 |
| ISSN | 1386-2073 |
| Publication status | Published - May 2002 |
Keywords
- Amino Acid Sequence
- Base Sequence
- Combinatorial Chemistry Techniques
- Escherichia coli/enzymology
- Gene Library
- Indicators and Reagents
- Membranes, Artificial
- Molecular Sequence Data
- Peptide Library
- Peptide Nucleic Acids/chemical synthesis
- Piperidines/pharmacology
- beta-Lactamases/genetics