Pancreatic beta-cell overexpression of the glucagon receptor gene results in enhanced beta-cell function and mass

Richard W Gelling; Patricia M Vuguin; Xiu Quan Du; Lingguang Cui; John Rømer; Raymond A Pederson; Margarita Leiser; Heidi Sørensen; Jens J Holst; Christian Fledelius; Peter B Johansen; Norman Fleischer; Christopher H S McIntosh; Erica Nishimura; Maureen J Charron

doi:10.1152/ajpendo.00082.2009

Pancreatic beta-cell overexpression of the glucagon receptor gene results in enhanced beta-cell function and mass

Richard W Gelling, Patricia M Vuguin, Xiu Quan Du, Lingguang Cui, John Rømer, Raymond A Pederson, Margarita Leiser, Heidi Sørensen, Jens J Holst, Christian Fledelius, Peter B Johansen, Norman Fleischer, Christopher H S McIntosh, Erica Nishimura, Maureen J Charron

Endocrinology and Metabolism

49 Citations (Scopus)

Abstract

In addition to its primary role in regulating glucose production from the liver, glucagon has many other actions, reflected by the wide tissue distribution of the glucagon receptor (Gcgr). To investigate the role of glucagon in the regulation of insulin secretion and whole body glucose homeostasis in vivo, we generated mice overexpressing the Gcgr specifically on pancreatic beta-cells (RIP-Gcgr). In vivo and in vitro insulin secretion in response to glucagon and glucose was increased 1.7- to 3.9-fold in RIP-Gcgr mice compared with controls. Consistent with the observed increase in insulin release in response to glucagon and glucose, the glucose excursion resulting from both a glucagon challenge and intraperitoneal glucose tolerance test (IPGTT) was significantly reduced in RIP-Gcgr mice compared with controls. However, RIP-Gcgr mice display similar glucose responses to an insulin challenge. beta-Cell mass and pancreatic insulin content were also increased (20 and 50%, respectively) in RIP-Gcgr mice compared with controls. When fed a high-fat diet (HFD), both control and RIP-Gcgr mice developed similar degrees of obesity and insulin resistance. However, the severity of both fasting hyperglycemia and impaired glucose tolerance (IGT) were reduced in RIP-Gcgr mice compared with controls. Furthermore, the insulin response of RIP-Gcgr mice to an IPGTT was twice that of controls when fed the HFD. These data indicate that increased pancreatic beta-cell expression of the Gcgr increased insulin secretion, pancreatic insulin content, beta-cell mass, and, when mice were fed a HFD, partially protected against hyperglycemia and IGT.

Original language	English
Journal	American Journal of Physiology: Endocrinology and Metabolism
Volume	297
Issue number	3
Pages (from-to)	E695-707
ISSN	0193-1849
DOIs	https://doi.org/10.1152/ajpendo.00082.2009
Publication status	Published - 2009

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Gelling, R. W., Vuguin, P. M., Du, X. Q., Cui, L., Rømer, J., Pederson, R. A., Leiser, M., Sørensen, H., Holst, J. J., Fledelius, C., Johansen, P. B., Fleischer, N., McIntosh, C. H. S., Nishimura, E., & Charron, M. J. (2009). Pancreatic beta-cell overexpression of the glucagon receptor gene results in enhanced beta-cell function and mass. American Journal of Physiology: Endocrinology and Metabolism, 297(3), E695-707. https://doi.org/10.1152/ajpendo.00082.2009

Gelling, RW, Vuguin, PM, Du, XQ, Cui, L, Rømer, J, Pederson, RA, Leiser, M, Sørensen, H, Holst, JJ, Fledelius, C, Johansen, PB, Fleischer, N, McIntosh, CHS, Nishimura, E & Charron, MJ 2009, 'Pancreatic beta-cell overexpression of the glucagon receptor gene results in enhanced beta-cell function and mass', American Journal of Physiology: Endocrinology and Metabolism, vol. 297, no. 3, pp. E695-707. https://doi.org/10.1152/ajpendo.00082.2009

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title = "Pancreatic beta-cell overexpression of the glucagon receptor gene results in enhanced beta-cell function and mass",

abstract = "In addition to its primary role in regulating glucose production from the liver, glucagon has many other actions, reflected by the wide tissue distribution of the glucagon receptor (Gcgr). To investigate the role of glucagon in the regulation of insulin secretion and whole body glucose homeostasis in vivo, we generated mice overexpressing the Gcgr specifically on pancreatic beta-cells (RIP-Gcgr). In vivo and in vitro insulin secretion in response to glucagon and glucose was increased 1.7- to 3.9-fold in RIP-Gcgr mice compared with controls. Consistent with the observed increase in insulin release in response to glucagon and glucose, the glucose excursion resulting from both a glucagon challenge and intraperitoneal glucose tolerance test (IPGTT) was significantly reduced in RIP-Gcgr mice compared with controls. However, RIP-Gcgr mice display similar glucose responses to an insulin challenge. beta-Cell mass and pancreatic insulin content were also increased (20 and 50%, respectively) in RIP-Gcgr mice compared with controls. When fed a high-fat diet (HFD), both control and RIP-Gcgr mice developed similar degrees of obesity and insulin resistance. However, the severity of both fasting hyperglycemia and impaired glucose tolerance (IGT) were reduced in RIP-Gcgr mice compared with controls. Furthermore, the insulin response of RIP-Gcgr mice to an IPGTT was twice that of controls when fed the HFD. These data indicate that increased pancreatic beta-cell expression of the Gcgr increased insulin secretion, pancreatic insulin content, beta-cell mass, and, when mice were fed a HFD, partially protected against hyperglycemia and IGT.",

author = "Gelling, {Richard W} and Vuguin, {Patricia M} and Du, {Xiu Quan} and Lingguang Cui and John R{\o}mer and Pederson, {Raymond A} and Margarita Leiser and Heidi S{\o}rensen and Holst, {Jens J} and Christian Fledelius and Johansen, {Peter B} and Norman Fleischer and McIntosh, {Christopher H S} and Erica Nishimura and Charron, {Maureen J}",

note = "Keywords: Animals; Cell Proliferation; Cell Size; Cells, Cultured; Diet, Atherogenic; Female; Glucose Intolerance; Hyperglycemia; Insulin; Insulin-Secreting Cells; Male; Mice; Mice, Inbred C57BL; Mice, Inbred CBA; Mice, Transgenic; Organ Specificity; Receptors, Glucagon; Transfection",

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doi = "10.1152/ajpendo.00082.2009",

language = "English",

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pages = "E695--707",

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T1 - Pancreatic beta-cell overexpression of the glucagon receptor gene results in enhanced beta-cell function and mass

AU - Gelling, Richard W

AU - Vuguin, Patricia M

AU - Du, Xiu Quan

AU - Cui, Lingguang

AU - Rømer, John

AU - Pederson, Raymond A

AU - Leiser, Margarita

AU - Sørensen, Heidi

AU - Holst, Jens J

AU - Fledelius, Christian

AU - Johansen, Peter B

AU - Fleischer, Norman

AU - McIntosh, Christopher H S

AU - Nishimura, Erica

AU - Charron, Maureen J

N1 - Keywords: Animals; Cell Proliferation; Cell Size; Cells, Cultured; Diet, Atherogenic; Female; Glucose Intolerance; Hyperglycemia; Insulin; Insulin-Secreting Cells; Male; Mice; Mice, Inbred C57BL; Mice, Inbred CBA; Mice, Transgenic; Organ Specificity; Receptors, Glucagon; Transfection

PY - 2009

Y1 - 2009

N2 - In addition to its primary role in regulating glucose production from the liver, glucagon has many other actions, reflected by the wide tissue distribution of the glucagon receptor (Gcgr). To investigate the role of glucagon in the regulation of insulin secretion and whole body glucose homeostasis in vivo, we generated mice overexpressing the Gcgr specifically on pancreatic beta-cells (RIP-Gcgr). In vivo and in vitro insulin secretion in response to glucagon and glucose was increased 1.7- to 3.9-fold in RIP-Gcgr mice compared with controls. Consistent with the observed increase in insulin release in response to glucagon and glucose, the glucose excursion resulting from both a glucagon challenge and intraperitoneal glucose tolerance test (IPGTT) was significantly reduced in RIP-Gcgr mice compared with controls. However, RIP-Gcgr mice display similar glucose responses to an insulin challenge. beta-Cell mass and pancreatic insulin content were also increased (20 and 50%, respectively) in RIP-Gcgr mice compared with controls. When fed a high-fat diet (HFD), both control and RIP-Gcgr mice developed similar degrees of obesity and insulin resistance. However, the severity of both fasting hyperglycemia and impaired glucose tolerance (IGT) were reduced in RIP-Gcgr mice compared with controls. Furthermore, the insulin response of RIP-Gcgr mice to an IPGTT was twice that of controls when fed the HFD. These data indicate that increased pancreatic beta-cell expression of the Gcgr increased insulin secretion, pancreatic insulin content, beta-cell mass, and, when mice were fed a HFD, partially protected against hyperglycemia and IGT.

AB - In addition to its primary role in regulating glucose production from the liver, glucagon has many other actions, reflected by the wide tissue distribution of the glucagon receptor (Gcgr). To investigate the role of glucagon in the regulation of insulin secretion and whole body glucose homeostasis in vivo, we generated mice overexpressing the Gcgr specifically on pancreatic beta-cells (RIP-Gcgr). In vivo and in vitro insulin secretion in response to glucagon and glucose was increased 1.7- to 3.9-fold in RIP-Gcgr mice compared with controls. Consistent with the observed increase in insulin release in response to glucagon and glucose, the glucose excursion resulting from both a glucagon challenge and intraperitoneal glucose tolerance test (IPGTT) was significantly reduced in RIP-Gcgr mice compared with controls. However, RIP-Gcgr mice display similar glucose responses to an insulin challenge. beta-Cell mass and pancreatic insulin content were also increased (20 and 50%, respectively) in RIP-Gcgr mice compared with controls. When fed a high-fat diet (HFD), both control and RIP-Gcgr mice developed similar degrees of obesity and insulin resistance. However, the severity of both fasting hyperglycemia and impaired glucose tolerance (IGT) were reduced in RIP-Gcgr mice compared with controls. Furthermore, the insulin response of RIP-Gcgr mice to an IPGTT was twice that of controls when fed the HFD. These data indicate that increased pancreatic beta-cell expression of the Gcgr increased insulin secretion, pancreatic insulin content, beta-cell mass, and, when mice were fed a HFD, partially protected against hyperglycemia and IGT.

U2 - 10.1152/ajpendo.00082.2009

DO - 10.1152/ajpendo.00082.2009

M3 - Journal article

C2 - 19602585

SN - 0193-1849

VL - 297

SP - E695-707

JO - American Journal of Physiology - Endocrinology and Metabolism

JF - American Journal of Physiology - Endocrinology and Metabolism

IS - 3

ER -

Pancreatic beta-cell overexpression of the glucagon receptor gene results in enhanced beta-cell function and mass

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