Palaeoproteomic Profiling of Conservation Layers on a 14th Century Italian Wall Painting

Meaghan Emma Mackie; Patrick Leopold Rüther; Diana Samodova; Fabiana Di Gianvincenzo; Clara Granzotto; David Lyon; David A. Peggie; Helen Howard; Lynne Harrison; Lars Juhl Jensen; Jesper Velgaard Olsen; Enrico Cappellini

doi:10.1002/anie.201713020

Palaeoproteomic Profiling of Conservation Layers on a 14th Century Italian Wall Painting

Meaghan Emma Mackie, Patrick Leopold Rüther, Diana Samodova, Fabiana Di Gianvincenzo, Clara Granzotto, David Lyon, David A. Peggie, Helen Howard, Lynne Harrison, Lars Juhl Jensen, Jesper Velgaard Olsen, Enrico Cappellini

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Abstract

Ahead of display, a non-original layer was observed on the surface of a fragment of a wall painting by Ambrogio Lorenzetti (active 1319, died 1348/9). FTIR analysis suggested proteinaceous content. Mass spectrometry was used to better characterise this layer and revealed two protein components: sheep and cow glue and chicken and duck egg white. Analysis of post-translational modifications detected several photo-oxidation products, which suggest that the egg experienced prolonged exposure to UV light and was likely applied long before the glue layer. Additionally, glycation products detected may indicate naturally occurring glycoprotein degradation or reaction with a carbohydrate material such as starch, identified by ATR-FTIR in a cross-section of a sample taken from the painting. Palaeoproteomics is shown to provide detailed characterisation of organic layers associated with mural paintings and therefore aids reconstruction of the conservation history of these objects.

Original language	English
Journal	Angewandte Chemie (International ed. in English)
Volume	57
Issue number	25
Pages (from-to)	7369-7374
Number of pages	6
ISSN	1433-7851
DOIs	https://doi.org/10.1002/anie.201713020
Publication status	Published - 18 Jun 2018

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Palaeoproteomic Profiling of Conservation Layers on a 14th Century Italian Wall PaintingFinal published version, 1.5 MBLicence: CC BY

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Mackie, M. E., Rüther, P. L., Samodova, D., Di Gianvincenzo, F., Granzotto, C., Lyon, D., Peggie, D. A., Howard, H., Harrison, L., Jensen, L. J., Olsen, J. V., & Cappellini, E. (2018). Palaeoproteomic Profiling of Conservation Layers on a 14th Century Italian Wall Painting. Angewandte Chemie (International ed. in English), 57(25), 7369-7374. https://doi.org/10.1002/anie.201713020

Mackie, ME , Rüther, PL , Samodova, D , Di Gianvincenzo, F, Granzotto, C, Lyon, D, Peggie, DA, Howard, H, Harrison, L, Jensen, LJ , Olsen, JV & Cappellini, E 2018, 'Palaeoproteomic Profiling of Conservation Layers on a 14th Century Italian Wall Painting', Angewandte Chemie (International ed. in English), vol. 57, no. 25, pp. 7369-7374. https://doi.org/10.1002/anie.201713020

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title = "Palaeoproteomic Profiling of Conservation Layers on a 14th Century Italian Wall Painting",

abstract = "Ahead of display, a non-original layer was observed on the surface of a fragment of a wall painting by Ambrogio Lorenzetti (active 1319, died 1348/9). FTIR analysis suggested proteinaceous content. Mass spectrometry was used to better characterise this layer and revealed two protein components: sheep and cow glue and chicken and duck egg white. Analysis of post-translational modifications detected several photo-oxidation products, which suggest that the egg experienced prolonged exposure to UV light and was likely applied long before the glue layer. Additionally, glycation products detected may indicate naturally occurring glycoprotein degradation or reaction with a carbohydrate material such as starch, identified by ATR-FTIR in a cross-section of a sample taken from the painting. Palaeoproteomics is shown to provide detailed characterisation of organic layers associated with mural paintings and therefore aids reconstruction of the conservation history of these objects.",

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AU - Mackie, Meaghan Emma

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AU - Di Gianvincenzo, Fabiana

AU - Granzotto, Clara

AU - Lyon, David

AU - Peggie, David A.

AU - Howard, Helen

AU - Harrison, Lynne

AU - Jensen, Lars Juhl

AU - Olsen, Jesper Velgaard

AU - Cappellini, Enrico

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N2 - Ahead of display, a non-original layer was observed on the surface of a fragment of a wall painting by Ambrogio Lorenzetti (active 1319, died 1348/9). FTIR analysis suggested proteinaceous content. Mass spectrometry was used to better characterise this layer and revealed two protein components: sheep and cow glue and chicken and duck egg white. Analysis of post-translational modifications detected several photo-oxidation products, which suggest that the egg experienced prolonged exposure to UV light and was likely applied long before the glue layer. Additionally, glycation products detected may indicate naturally occurring glycoprotein degradation or reaction with a carbohydrate material such as starch, identified by ATR-FTIR in a cross-section of a sample taken from the painting. Palaeoproteomics is shown to provide detailed characterisation of organic layers associated with mural paintings and therefore aids reconstruction of the conservation history of these objects.

AB - Ahead of display, a non-original layer was observed on the surface of a fragment of a wall painting by Ambrogio Lorenzetti (active 1319, died 1348/9). FTIR analysis suggested proteinaceous content. Mass spectrometry was used to better characterise this layer and revealed two protein components: sheep and cow glue and chicken and duck egg white. Analysis of post-translational modifications detected several photo-oxidation products, which suggest that the egg experienced prolonged exposure to UV light and was likely applied long before the glue layer. Additionally, glycation products detected may indicate naturally occurring glycoprotein degradation or reaction with a carbohydrate material such as starch, identified by ATR-FTIR in a cross-section of a sample taken from the painting. Palaeoproteomics is shown to provide detailed characterisation of organic layers associated with mural paintings and therefore aids reconstruction of the conservation history of these objects.

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DO - 10.1002/anie.201713020

M3 - Journal article

C2 - 29603563

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JO - Angewandte Chemie (International ed. in English)

JF - Angewandte Chemie (International ed. in English)

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ER -

Palaeoproteomic Profiling of Conservation Layers on a 14th Century Italian Wall Painting

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