TY - JOUR
T1 - Palaeoproteomic Profiling of Conservation Layers on a 14th Century Italian Wall Painting
AU - Mackie, Meaghan Emma
AU - Rüther, Patrick Leopold
AU - Samodova, Diana
AU - Di Gianvincenzo, Fabiana
AU - Granzotto, Clara
AU - Lyon, David
AU - Peggie, David A.
AU - Howard, Helen
AU - Harrison, Lynne
AU - Jensen, Lars Juhl
AU - Olsen, Jesper Velgaard
AU - Cappellini, Enrico
N1 - © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
PY - 2018/6/18
Y1 - 2018/6/18
N2 - Ahead of display, a non-original layer was observed on the surface of a fragment of a wall painting by Ambrogio Lorenzetti (active 1319, died 1348/9). FTIR analysis suggested proteinaceous content. Mass spectrometry was used to better characterise this layer and revealed two protein components: sheep and cow glue and chicken and duck egg white. Analysis of post-translational modifications detected several photo-oxidation products, which suggest that the egg experienced prolonged exposure to UV light and was likely applied long before the glue layer. Additionally, glycation products detected may indicate naturally occurring glycoprotein degradation or reaction with a carbohydrate material such as starch, identified by ATR-FTIR in a cross-section of a sample taken from the painting. Palaeoproteomics is shown to provide detailed characterisation of organic layers associated with mural paintings and therefore aids reconstruction of the conservation history of these objects.
AB - Ahead of display, a non-original layer was observed on the surface of a fragment of a wall painting by Ambrogio Lorenzetti (active 1319, died 1348/9). FTIR analysis suggested proteinaceous content. Mass spectrometry was used to better characterise this layer and revealed two protein components: sheep and cow glue and chicken and duck egg white. Analysis of post-translational modifications detected several photo-oxidation products, which suggest that the egg experienced prolonged exposure to UV light and was likely applied long before the glue layer. Additionally, glycation products detected may indicate naturally occurring glycoprotein degradation or reaction with a carbohydrate material such as starch, identified by ATR-FTIR in a cross-section of a sample taken from the painting. Palaeoproteomics is shown to provide detailed characterisation of organic layers associated with mural paintings and therefore aids reconstruction of the conservation history of these objects.
U2 - 10.1002/anie.201713020
DO - 10.1002/anie.201713020
M3 - Journal article
C2 - 29603563
SN - 1433-7851
VL - 57
SP - 7369
EP - 7374
JO - Angewandte Chemie International Edition
JF - Angewandte Chemie International Edition
IS - 25
ER -
