Overproduction of PIB-Type ATPases

Xiangyu Liu; Oleg Sitsel; Kaituo Wang; Pontus Gourdon

doi:10.1007/978-1-4939-3179-8_5

Overproduction of PIB-Type ATPases

Xiangyu Liu, Oleg Sitsel, Kaituo Wang, Pontus Gourdon

Inflammation, Metabolism and Oxidation

Abstract

Understanding of the functions and mechanisms of fundamental processes in the cell requires structural information. Structural studies of membrane proteins typically necessitate large amounts of purified and preferably homogenous target protein. Here, we describe a rapid overproduction and purification strategy of a bacterial PIB-type ATPase for isolation of milligrams of target protein per liter Escherichia coli cell culture, with a final quality of the sample which is sufficient for generating high-resolution crystals.

Original language	English
Title of host publication	P-Type ATPases : Methods and Protocols
Editors	Maike Bublitz
Number of pages	8
Volume	1377
Publisher	Springer
Publication date	1 Jan 2016
Pages	29-36
Chapter	5
ISBN (Print)	978-1-4939-3178-1
ISBN (Electronic)	978-1-4939-3179-8
DOIs	https://doi.org/10.1007/978-1-4939-3179-8_5
Publication status	Published - 1 Jan 2016

Series	Methods in Molecular Biology
Volume	1377
ISSN	1064-3745

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10.1007/978-1-4939-3179-8_5

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Overproduction of PIB-Type ATPases

Abstract

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