Overproduction of PIB-Type ATPases

TY - CHAP

T1 - Overproduction of PIB-Type ATPases

AU - Liu, Xiangyu

AU - Sitsel, Oleg

AU - Wang, Kaituo

AU - Gourdon, Pontus

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Understanding of the functions and mechanisms of fundamental processes in the cell requires structural information. Structural studies of membrane proteins typically necessitate large amounts of purified and preferably homogenous target protein. Here, we describe a rapid overproduction and purification strategy of a bacterial PIB-type ATPase for isolation of milligrams of target protein per liter Escherichia coli cell culture, with a final quality of the sample which is sufficient for generating high-resolution crystals.

AB - Understanding of the functions and mechanisms of fundamental processes in the cell requires structural information. Structural studies of membrane proteins typically necessitate large amounts of purified and preferably homogenous target protein. Here, we describe a rapid overproduction and purification strategy of a bacterial PIB-type ATPase for isolation of milligrams of target protein per liter Escherichia coli cell culture, with a final quality of the sample which is sufficient for generating high-resolution crystals.

U2 - 10.1007/978-1-4939-3179-8_5

DO - 10.1007/978-1-4939-3179-8_5

M3 - Book chapter

C2 - 26695020

SN - 978-1-4939-3178-1

VL - 1377

T3 - Methods in Molecular Biology

SP - 29

EP - 36

BT - P-Type ATPases

A2 - Bublitz, Maike

PB - Springer

ER -