Optimization of RNA preservation in granulation tissue in the expanded polytetrafluoroethylene (ePTFE) tube model

TY - JOUR

T1 - Optimization of RNA preservation in granulation tissue in the expanded polytetrafluoroethylene (ePTFE) tube model

AU - Danielsen, Patricia L

AU - Koudahl, V

AU - Christensen, B

AU - Olsen, Jørgen

PY - 2009

Y1 - 2009

N2 - RNA is easily degraded by RNAses. An efficient and non-hazardous method optimizing RNA preservation and extraction is needed. Our aim was to evaluate RNA preservation methods on murine granulation tissue from subcutaneously implanted ePTFE tubes. ePTFE tubes were placed subcutaneously in mice, removed after ten days and randomized to four RNA preservation groups. Storage in RNAlater at 4 degrees C and snap freezing in methanol/acetone yielded significantly better RNA quality than snap freezing with liquid nitrogen and storage in RNAlater at -80 degrees C. Snap freezing in liquid nitrogen failed to yield intact RNA. We recommend storage in RNAlater at 4 degrees C.

AB - RNA is easily degraded by RNAses. An efficient and non-hazardous method optimizing RNA preservation and extraction is needed. Our aim was to evaluate RNA preservation methods on murine granulation tissue from subcutaneously implanted ePTFE tubes. ePTFE tubes were placed subcutaneously in mice, removed after ten days and randomized to four RNA preservation groups. Storage in RNAlater at 4 degrees C and snap freezing in methanol/acetone yielded significantly better RNA quality than snap freezing with liquid nitrogen and storage in RNAlater at -80 degrees C. Snap freezing in liquid nitrogen failed to yield intact RNA. We recommend storage in RNAlater at 4 degrees C.

U2 - 10.1080/10826060903209695

DO - 10.1080/10826060903209695

M3 - Journal article

C2 - 19739027

SN - 1082-6068

VL - 39

SP - 413

EP - 418

JO - Preparative Biochemistry and Biotechnology

JF - Preparative Biochemistry and Biotechnology

IS - 4

ER -