Optical and Hydrodynamic Stretching of Single Cells from Blood

Henrik Thirstrup; Tony B. Rungling; Mustafa Zyad Khalil Al-Hamdani; Ragavan Pathanchalinathan; Morten Hanefeld Dziegiel; Anders Kristensen; Rodolphe Marie; Kirstine Berg-Sørensen

doi:10.1364/OTA.2017.OtM4E.1

Optical and Hydrodynamic Stretching of Single Cells from Blood

Henrik Thirstrup, Tony B. Rungling, Mustafa Zyad Khalil Al-Hamdani, Ragavan Pathanchalinathan, Morten Hanefeld Dziegiel, Anders Kristensen, Rodolphe Marie, Kirstine Berg-Sørensen^*

^*Corresponding author for this work

Department of Clinical Medicine

Abstract

Mechanical properties, like deformability or elasticity, of cells can in some cases be indicative of the health of the organism they originate from. In this work, we explore the potential of deformability and other mechanical parameters of individual red blood cells (RBCs) from humans as a marker for the state of health of the human source, patient or donor. In particular, we have investigated the use of different experimental strategies implemented in injection molded plastic microfluidic devices. One strategy is to optically stretch the red blood cells in an optical two-beam trap, also known as an optical stretcher, in a microfluidic chip in which optical fibers have been placed during a post-processing step. Another strategy is to exert hydrodynamic shear forces on the cells by forcing the cells through a narrow constriction. The latter method has the advantage of a considerably higher throughput but does so far not allow for subsequent investigations of single "interesting" cells. The paper is a progress report with preliminary results based on the different strategies, we have pursued.

Original language	English
Title of host publication	Optical Trapping Applications, OTA 2017
Volume	Part F64-OTA 2017
Publisher	The Optical Society (OSA)
Publication date	1 Jan 2017
Article number	OtM4E.1
ISBN (Electronic)	9781557528209
DOIs	https://doi.org/10.1364/OTA.2017.OtM4E.1
Publication status	Published - 1 Jan 2017
Event	Optical Trapping Applications, OTA 2017 - San Diego, United States Duration: 2 Apr 2017 → 5 Apr 2017

Conference

Conference	Optical Trapping Applications, OTA 2017
Country/Territory	United States
City	San Diego
Period	02/04/2017 → 05/04/2017
Sponsor	OSA

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10.1364/OTA.2017.OtM4E.1

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Thirstrup, H, Rungling, TB, Khalil Al-Hamdani, MZ, Pathanchalinathan, R, Dziegiel, MH, Kristensen, A, Marie, R & Berg-Sørensen, K 2017, Optical and Hydrodynamic Stretching of Single Cells from Blood. in Optical Trapping Applications, OTA 2017. vol. Part F64-OTA 2017, OtM4E.1, The Optical Society (OSA), Optical Trapping Applications, OTA 2017, San Diego, United States, 02/04/2017. https://doi.org/10.1364/OTA.2017.OtM4E.1

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abstract = "Mechanical properties, like deformability or elasticity, of cells can in some cases be indicative of the health of the organism they originate from. In this work, we explore the potential of deformability and other mechanical parameters of individual red blood cells (RBCs) from humans as a marker for the state of health of the human source, patient or donor. In particular, we have investigated the use of different experimental strategies implemented in injection molded plastic microfluidic devices. One strategy is to optically stretch the red blood cells in an optical two-beam trap, also known as an optical stretcher, in a microfluidic chip in which optical fibers have been placed during a post-processing step. Another strategy is to exert hydrodynamic shear forces on the cells by forcing the cells through a narrow constriction. The latter method has the advantage of a considerably higher throughput but does so far not allow for subsequent investigations of single {"}interesting{"} cells. The paper is a progress report with preliminary results based on the different strategies, we have pursued.",

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AU - Thirstrup, Henrik

AU - Rungling, Tony B.

AU - Khalil Al-Hamdani, Mustafa Zyad

AU - Pathanchalinathan, Ragavan

AU - Dziegiel, Morten Hanefeld

AU - Kristensen, Anders

AU - Marie, Rodolphe

AU - Berg-Sørensen, Kirstine

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N2 - Mechanical properties, like deformability or elasticity, of cells can in some cases be indicative of the health of the organism they originate from. In this work, we explore the potential of deformability and other mechanical parameters of individual red blood cells (RBCs) from humans as a marker for the state of health of the human source, patient or donor. In particular, we have investigated the use of different experimental strategies implemented in injection molded plastic microfluidic devices. One strategy is to optically stretch the red blood cells in an optical two-beam trap, also known as an optical stretcher, in a microfluidic chip in which optical fibers have been placed during a post-processing step. Another strategy is to exert hydrodynamic shear forces on the cells by forcing the cells through a narrow constriction. The latter method has the advantage of a considerably higher throughput but does so far not allow for subsequent investigations of single "interesting" cells. The paper is a progress report with preliminary results based on the different strategies, we have pursued.

AB - Mechanical properties, like deformability or elasticity, of cells can in some cases be indicative of the health of the organism they originate from. In this work, we explore the potential of deformability and other mechanical parameters of individual red blood cells (RBCs) from humans as a marker for the state of health of the human source, patient or donor. In particular, we have investigated the use of different experimental strategies implemented in injection molded plastic microfluidic devices. One strategy is to optically stretch the red blood cells in an optical two-beam trap, also known as an optical stretcher, in a microfluidic chip in which optical fibers have been placed during a post-processing step. Another strategy is to exert hydrodynamic shear forces on the cells by forcing the cells through a narrow constriction. The latter method has the advantage of a considerably higher throughput but does so far not allow for subsequent investigations of single "interesting" cells. The paper is a progress report with preliminary results based on the different strategies, we have pursued.

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M3 - Article in proceedings

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VL - Part F64-OTA 2017

BT - Optical Trapping Applications, OTA 2017

PB - The Optical Society (OSA)

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Y2 - 2 April 2017 through 5 April 2017

ER -

Optical and Hydrodynamic Stretching of Single Cells from Blood

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