Optical and Hydrodynamic Stretching of Single Cells from Blood

TY - GEN

T1 - Optical and Hydrodynamic Stretching of Single Cells from Blood

AU - Thirstrup, Henrik

AU - Rungling, Tony B.

AU - Khalil Al-Hamdani, Mustafa Zyad

AU - Pathanchalinathan, Ragavan

AU - Dziegiel, Morten Hanefeld

AU - Kristensen, Anders

AU - Marie, Rodolphe

AU - Berg-Sørensen, Kirstine

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Mechanical properties, like deformability or elasticity, of cells can in some cases be indicative of the health of the organism they originate from. In this work, we explore the potential of deformability and other mechanical parameters of individual red blood cells (RBCs) from humans as a marker for the state of health of the human source, patient or donor. In particular, we have investigated the use of different experimental strategies implemented in injection molded plastic microfluidic devices. One strategy is to optically stretch the red blood cells in an optical two-beam trap, also known as an optical stretcher, in a microfluidic chip in which optical fibers have been placed during a post-processing step. Another strategy is to exert hydrodynamic shear forces on the cells by forcing the cells through a narrow constriction. The latter method has the advantage of a considerably higher throughput but does so far not allow for subsequent investigations of single "interesting" cells. The paper is a progress report with preliminary results based on the different strategies, we have pursued.

AB - Mechanical properties, like deformability or elasticity, of cells can in some cases be indicative of the health of the organism they originate from. In this work, we explore the potential of deformability and other mechanical parameters of individual red blood cells (RBCs) from humans as a marker for the state of health of the human source, patient or donor. In particular, we have investigated the use of different experimental strategies implemented in injection molded plastic microfluidic devices. One strategy is to optically stretch the red blood cells in an optical two-beam trap, also known as an optical stretcher, in a microfluidic chip in which optical fibers have been placed during a post-processing step. Another strategy is to exert hydrodynamic shear forces on the cells by forcing the cells through a narrow constriction. The latter method has the advantage of a considerably higher throughput but does so far not allow for subsequent investigations of single "interesting" cells. The paper is a progress report with preliminary results based on the different strategies, we have pursued.

UR - http://www.scopus.com/inward/record.url?scp=85032292055&partnerID=8YFLogxK

U2 - 10.1364/OTA.2017.OtM4E.1

DO - 10.1364/OTA.2017.OtM4E.1

M3 - Article in proceedings

AN - SCOPUS:85032292055

VL - Part F64-OTA 2017

BT - Optical Trapping Applications, OTA 2017

PB - The Optical Society (OSA)

T2 - Optical Trapping Applications, OTA 2017

Y2 - 2 April 2017 through 5 April 2017

ER -