Oligonucleotides Containing Aminated 2′-Amino-LNA Nucleotides: Synthesis and Strong Binding to Complementary DNA and RNA

Chenguang Lou; Simone V. Samuelsen; Niels Johan Christensen; Birte Vester; Jesper Wengel

doi:10.1021/acs.bioconjchem.7b00061

Oligonucleotides Containing Aminated 2′-Amino-LNA Nucleotides: Synthesis and Strong Binding to Complementary DNA and RNA

Chenguang Lou, Simone V. Samuelsen, Niels Johan Christensen, Birte Vester, Jesper Wengel^*

^*Corresponding author for this work

Department of Chemistry

11 Citations (Scopus)

Abstract

Mono- and diaminated 2′-amino-LNA monomers were synthesized and introduced into oligonucleotides. Each modification imparts significant stabilization of nucleic acid duplexes and triplexes, excellent sequence selectivity, and significant nuclease resistance. Molecular modeling suggested that structural stabilization occurs via intrastrand electrostatic attraction between the protonated amino groups of the aminated 2′-amino-LNA monomers and the host oligonucleotide backbone.

Original language	English
Journal	Bioconjugate Chemistry
Volume	28
Issue number	4
Pages (from-to)	1214-1220
Number of pages	7
ISSN	1043-1802
DOIs	https://doi.org/10.1021/acs.bioconjchem.7b00061
Publication status	Published - 2017

Access to Document

10.1021/acs.bioconjchem.7b00061

Cite this

@article{7c9a0b3dce9f49169b9af2ea3cc48bb6,

title = "Oligonucleotides Containing Aminated 2′-Amino-LNA Nucleotides: Synthesis and Strong Binding to Complementary DNA and RNA",

abstract = "Mono- and diaminated 2′-amino-LNA monomers were synthesized and introduced into oligonucleotides. Each modification imparts significant stabilization of nucleic acid duplexes and triplexes, excellent sequence selectivity, and significant nuclease resistance. Molecular modeling suggested that structural stabilization occurs via intrastrand electrostatic attraction between the protonated amino groups of the aminated 2′-amino-LNA monomers and the host oligonucleotide backbone.",

author = "Chenguang Lou and Samuelsen, {Simone V.} and Christensen, {Niels Johan} and Birte Vester and Jesper Wengel",

year = "2017",

doi = "10.1021/acs.bioconjchem.7b00061",

language = "English",

volume = "28",

pages = "1214--1220",

journal = "Bioconjugate Chemistry",

issn = "1043-1802",

publisher = "American Chemical Society",

number = "4",

}

TY - JOUR

T1 - Oligonucleotides Containing Aminated 2′-Amino-LNA Nucleotides

T2 - Synthesis and Strong Binding to Complementary DNA and RNA

AU - Lou, Chenguang

AU - Samuelsen, Simone V.

AU - Christensen, Niels Johan

AU - Vester, Birte

AU - Wengel, Jesper

PY - 2017

Y1 - 2017

N2 - Mono- and diaminated 2′-amino-LNA monomers were synthesized and introduced into oligonucleotides. Each modification imparts significant stabilization of nucleic acid duplexes and triplexes, excellent sequence selectivity, and significant nuclease resistance. Molecular modeling suggested that structural stabilization occurs via intrastrand electrostatic attraction between the protonated amino groups of the aminated 2′-amino-LNA monomers and the host oligonucleotide backbone.

AB - Mono- and diaminated 2′-amino-LNA monomers were synthesized and introduced into oligonucleotides. Each modification imparts significant stabilization of nucleic acid duplexes and triplexes, excellent sequence selectivity, and significant nuclease resistance. Molecular modeling suggested that structural stabilization occurs via intrastrand electrostatic attraction between the protonated amino groups of the aminated 2′-amino-LNA monomers and the host oligonucleotide backbone.

U2 - 10.1021/acs.bioconjchem.7b00061

DO - 10.1021/acs.bioconjchem.7b00061

M3 - Journal article

C2 - 28332825

AN - SCOPUS:85018495237

SN - 1043-1802

VL - 28

SP - 1214

EP - 1220

JO - Bioconjugate Chemistry

JF - Bioconjugate Chemistry

IS - 4

ER -

Oligonucleotides Containing Aminated 2′-Amino-LNA Nucleotides: Synthesis and Strong Binding to Complementary DNA and RNA

Abstract

Access to Document

Fingerprint

Cite this