Nuclear magnetic resonance-based metabolomics reveals that dairy protein fractions affect urinary urea excretion differently in overweight adolescents

Hong Zheng; Christian C. Yde; Trine K. Dalsgaard; Karina Arnberg; Christian Mølgaard; Kim F. Michaelsen; Anni Larnkjær; Hanne C. Bertram

doi:10.1007/s00217-014-2347-0

Nuclear magnetic resonance-based metabolomics reveals that dairy protein fractions affect urinary urea excretion differently in overweight adolescents

Hong Zheng, Christian C. Yde, Trine K. Dalsgaard, Karina Arnberg, Christian Mølgaard, Kim F. Michaelsen, Anni Larnkjær, Hanne C. Bertram^*

^*Corresponding author for this work

Lifecourse Nutrition & Health

5 Citations (Scopus)

Abstract

Dairy proteins are an important part of our diet, and recently, there is considerable focus on understanding the effects of the two major dairy proteins fractions constituted by casein and whey. In the present study, the impact of a dietary intervention with casein, whey, and skim milk was investigated by using NMR-based urine metabolomics. Overweight adolescents (n = 192; age = 12–15 years; BMI = 25.4 ± 2.3 kg/m²) were randomly assigned to 1 L/day of casein (citrate content: 3.27 mol/L), whey (citrate content: 0.04 mol/L), skim milk, or water for 12 weeks. A significant increase in the urinary excretion of urea was found after the 12-week casein and skim milk interventions, while the 12-week whey intervention had no significant effect on the urea excretion. In addition, NMR-based metabolomics revealed a decreased urinary citrate excretion in the whey group and thereby demonstrated its potential to disclosure exposure effects. In conclusion, the present study demonstrates that casein and whey proteins impact urinary urea excretion differently.

Original language	English
Journal	European Food Research and Technology
Volume	240
Issue number	3
Pages (from-to)	489-497
Number of pages	9
ISSN	1438-2377
DOIs	https://doi.org/10.1007/s00217-014-2347-0
Publication status	Published - Mar 2014

Keywords

Casein
Citrate
Metabolomics
Milk protein
Urea
Whey

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10.1007/s00217-014-2347-0

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Zheng, H., Yde, C. C., Dalsgaard, T. K., Arnberg, K., Mølgaard, C., Michaelsen, K. F., Larnkjær, A., & Bertram, H. C. (2014). Nuclear magnetic resonance-based metabolomics reveals that dairy protein fractions affect urinary urea excretion differently in overweight adolescents. European Food Research and Technology, 240(3), 489-497. https://doi.org/10.1007/s00217-014-2347-0

Nuclear magnetic resonance-based metabolomics reveals that dairy protein fractions affect urinary urea excretion differently in overweight adolescents. / Zheng, Hong; Yde, Christian C.; Dalsgaard, Trine K. et al.

In: European Food Research and Technology, Vol. 240, No. 3, 03.2014, p. 489-497.

Research output: Contribution to journal › Journal article › Research › peer-review

Zheng, H, Yde, CC, Dalsgaard, TK, Arnberg, K, Mølgaard, C , Michaelsen, KF , Larnkjær, A & Bertram, HC 2014, 'Nuclear magnetic resonance-based metabolomics reveals that dairy protein fractions affect urinary urea excretion differently in overweight adolescents', European Food Research and Technology, vol. 240, no. 3, pp. 489-497. https://doi.org/10.1007/s00217-014-2347-0

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abstract = "Dairy proteins are an important part of our diet, and recently, there is considerable focus on understanding the effects of the two major dairy proteins fractions constituted by casein and whey. In the present study, the impact of a dietary intervention with casein, whey, and skim milk was investigated by using NMR-based urine metabolomics. Overweight adolescents (n = 192; age = 12–15 years; BMI = 25.4 ± 2.3 kg/m2) were randomly assigned to 1 L/day of casein (citrate content: 3.27 mol/L), whey (citrate content: 0.04 mol/L), skim milk, or water for 12 weeks. A significant increase in the urinary excretion of urea was found after the 12-week casein and skim milk interventions, while the 12-week whey intervention had no significant effect on the urea excretion. In addition, NMR-based metabolomics revealed a decreased urinary citrate excretion in the whey group and thereby demonstrated its potential to disclosure exposure effects. In conclusion, the present study demonstrates that casein and whey proteins impact urinary urea excretion differently.",

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AU - Zheng, Hong

AU - Yde, Christian C.

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AU - Arnberg, Karina

AU - Mølgaard, Christian

AU - Michaelsen, Kim F.

AU - Larnkjær, Anni

AU - Bertram, Hanne C.

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N2 - Dairy proteins are an important part of our diet, and recently, there is considerable focus on understanding the effects of the two major dairy proteins fractions constituted by casein and whey. In the present study, the impact of a dietary intervention with casein, whey, and skim milk was investigated by using NMR-based urine metabolomics. Overweight adolescents (n = 192; age = 12–15 years; BMI = 25.4 ± 2.3 kg/m2) were randomly assigned to 1 L/day of casein (citrate content: 3.27 mol/L), whey (citrate content: 0.04 mol/L), skim milk, or water for 12 weeks. A significant increase in the urinary excretion of urea was found after the 12-week casein and skim milk interventions, while the 12-week whey intervention had no significant effect on the urea excretion. In addition, NMR-based metabolomics revealed a decreased urinary citrate excretion in the whey group and thereby demonstrated its potential to disclosure exposure effects. In conclusion, the present study demonstrates that casein and whey proteins impact urinary urea excretion differently.

AB - Dairy proteins are an important part of our diet, and recently, there is considerable focus on understanding the effects of the two major dairy proteins fractions constituted by casein and whey. In the present study, the impact of a dietary intervention with casein, whey, and skim milk was investigated by using NMR-based urine metabolomics. Overweight adolescents (n = 192; age = 12–15 years; BMI = 25.4 ± 2.3 kg/m2) were randomly assigned to 1 L/day of casein (citrate content: 3.27 mol/L), whey (citrate content: 0.04 mol/L), skim milk, or water for 12 weeks. A significant increase in the urinary excretion of urea was found after the 12-week casein and skim milk interventions, while the 12-week whey intervention had no significant effect on the urea excretion. In addition, NMR-based metabolomics revealed a decreased urinary citrate excretion in the whey group and thereby demonstrated its potential to disclosure exposure effects. In conclusion, the present study demonstrates that casein and whey proteins impact urinary urea excretion differently.

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KW - Citrate

KW - Metabolomics

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KW - Urea

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DO - 10.1007/s00217-014-2347-0

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VL - 240

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EP - 497

JO - European Food Research and Technology

JF - European Food Research and Technology

IS - 3

ER -

Nuclear magnetic resonance-based metabolomics reveals that dairy protein fractions affect urinary urea excretion differently in overweight adolescents

Abstract

Keywords

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